Abstract
The menisci of the knee are semilunar fibrocartilaginous structures critical in load bearing, shock absorption, stability, and lubrication. In this study, two commonly used biomaterials, a hydrogel (agarose) and a nonwoven mesh polymer [poly(glycolic acid); PGA], were compared for suitability as scaffold materials for tissue engineering the knee meniscus. In addition, a rotating wall bioreactor culture of both scaffold materials was compared with static cultures. Constructs were cultured for up to 7 weeks in static and rotating wall bioreactor culture. Cell numbers were 22 times higher in PGA than agarose after 7 weeks in culture. Static PGA scaffolds had more than twice the amount of sulfated glycosaminoglycans and three times the amount of collagen compared to static agarose constructs at week 7. The rotating wall bioreactor was not found with increase matrix production or cell proliferation significantly over static cultures.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1095-1104 |
| Number of pages | 10 |
| Journal | Tissue Engineering |
| Volume | 11 |
| Issue number | 7-8 |
| DOIs | |
| State | Published - Jul 2005 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biophysics
- Cell Biology
- Biotechnology
Cite this
Comparison of scaffolds and culture conditions for tissue engineering of the knee meniscus. / Aufderheide, Adam C.; Athanasiou, Kyriacos A.
In: Tissue Engineering, Vol. 11, No. 7-8, 07.2005, p. 1095-1104.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of scaffolds and culture conditions for tissue engineering of the knee meniscus
AU - Aufderheide, Adam C.
AU - Athanasiou, Kyriacos A.
PY - 2005/7
Y1 - 2005/7
N2 - The menisci of the knee are semilunar fibrocartilaginous structures critical in load bearing, shock absorption, stability, and lubrication. In this study, two commonly used biomaterials, a hydrogel (agarose) and a nonwoven mesh polymer [poly(glycolic acid); PGA], were compared for suitability as scaffold materials for tissue engineering the knee meniscus. In addition, a rotating wall bioreactor culture of both scaffold materials was compared with static cultures. Constructs were cultured for up to 7 weeks in static and rotating wall bioreactor culture. Cell numbers were 22 times higher in PGA than agarose after 7 weeks in culture. Static PGA scaffolds had more than twice the amount of sulfated glycosaminoglycans and three times the amount of collagen compared to static agarose constructs at week 7. The rotating wall bioreactor was not found with increase matrix production or cell proliferation significantly over static cultures.
AB - The menisci of the knee are semilunar fibrocartilaginous structures critical in load bearing, shock absorption, stability, and lubrication. In this study, two commonly used biomaterials, a hydrogel (agarose) and a nonwoven mesh polymer [poly(glycolic acid); PGA], were compared for suitability as scaffold materials for tissue engineering the knee meniscus. In addition, a rotating wall bioreactor culture of both scaffold materials was compared with static cultures. Constructs were cultured for up to 7 weeks in static and rotating wall bioreactor culture. Cell numbers were 22 times higher in PGA than agarose after 7 weeks in culture. Static PGA scaffolds had more than twice the amount of sulfated glycosaminoglycans and three times the amount of collagen compared to static agarose constructs at week 7. The rotating wall bioreactor was not found with increase matrix production or cell proliferation significantly over static cultures.
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UR - http://www.scopus.com/inward/citedby.url?scp=24944436243&partnerID=8YFLogxK
U2 - 10.1089/ten.2005.11.1095
DO - 10.1089/ten.2005.11.1095
M3 - Article
C2 - 16144445
AN - SCOPUS:24944436243
VL - 11
SP - 1095
EP - 1104
JO - Tissue Engineering
JF - Tissue Engineering
SN - 1076-3279
IS - 7-8
ER -