Comparison of scaffolds and culture conditions for tissue engineering of the knee meniscus

Adam C. Aufderheide, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The menisci of the knee are semilunar fibrocartilaginous structures critical in load bearing, shock absorption, stability, and lubrication. In this study, two commonly used biomaterials, a hydrogel (agarose) and a nonwoven mesh polymer [poly(glycolic acid); PGA], were compared for suitability as scaffold materials for tissue engineering the knee meniscus. In addition, a rotating wall bioreactor culture of both scaffold materials was compared with static cultures. Constructs were cultured for up to 7 weeks in static and rotating wall bioreactor culture. Cell numbers were 22 times higher in PGA than agarose after 7 weeks in culture. Static PGA scaffolds had more than twice the amount of sulfated glycosaminoglycans and three times the amount of collagen compared to static agarose constructs at week 7. The rotating wall bioreactor was not found with increase matrix production or cell proliferation significantly over static cultures.

Original languageEnglish (US)
Pages (from-to)1095-1104
Number of pages10
JournalTissue Engineering
Volume11
Issue number7-8
DOIs
StatePublished - Jul 2005
Externally publishedYes

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Prostaglandins A
Bioreactors
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Sepharose
Knee
glycolic acid
A73025
Scaffolds
Bearings (structural)
Lubrication
Hydrogel
Weight-Bearing
Cell proliferation
Biocompatible Materials
Cell culture
Collagen
Biomaterials
Hydrogels

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Biotechnology

Cite this

Comparison of scaffolds and culture conditions for tissue engineering of the knee meniscus. / Aufderheide, Adam C.; Athanasiou, Kyriacos A.

In: Tissue Engineering, Vol. 11, No. 7-8, 07.2005, p. 1095-1104.

Research output: Contribution to journalArticle

Aufderheide, Adam C. ; Athanasiou, Kyriacos A. / Comparison of scaffolds and culture conditions for tissue engineering of the knee meniscus. In: Tissue Engineering. 2005 ; Vol. 11, No. 7-8. pp. 1095-1104.
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