Comparison of sampling methods for profiling cervicovaginal microbiome in rhesus macaques

Brian A. Schmidt; Ronald Phillips; Matthew Rolston; Reben Raeman; Smita Iyer

doi:10.1111/jmp.12381

Comparison of sampling methods for profiling cervicovaginal microbiome in rhesus macaques

Brian A. Schmidt, Ronald Phillips, Matthew Rolston, Reben Raeman, Smita Iyer

Pathology and Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Cervicovaginal bacteria cause inflammation which in turn increases HIV risk. Profiling the cervicovaginal microbiome, therefore, is instrumental for vaccine development. We show that the microbiome profile captured by cervicovaginal lavage is comparable to samples obtained by vaginal swabs. Thus, lavage may serve as a sampling strategy in NHP vaccine studies.

Original language	English (US)
Journal	Journal of Medical Primatology
DOIs	https://doi.org/10.1111/jmp.12381
State	Accepted/In press - Jan 1 2018

Keywords

16S rRNA
HIV
lavage
swab

ASJC Scopus subject areas

Animal Science and Zoology
veterinary(all)

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10.1111/jmp.12381

Cite this

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title = "Comparison of sampling methods for profiling cervicovaginal microbiome in rhesus macaques",

abstract = "Cervicovaginal bacteria cause inflammation which in turn increases HIV risk. Profiling the cervicovaginal microbiome, therefore, is instrumental for vaccine development. We show that the microbiome profile captured by cervicovaginal lavage is comparable to samples obtained by vaginal swabs. Thus, lavage may serve as a sampling strategy in NHP vaccine studies.",

keywords = "16S rRNA, HIV, lavage, swab",

author = "Schmidt, {Brian A.} and Ronald Phillips and Matthew Rolston and Reben Raeman and Smita Iyer",

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AU - Schmidt, Brian A.

AU - Phillips, Ronald

AU - Rolston, Matthew

AU - Raeman, Reben

AU - Iyer, Smita

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Cervicovaginal bacteria cause inflammation which in turn increases HIV risk. Profiling the cervicovaginal microbiome, therefore, is instrumental for vaccine development. We show that the microbiome profile captured by cervicovaginal lavage is comparable to samples obtained by vaginal swabs. Thus, lavage may serve as a sampling strategy in NHP vaccine studies.

AB - Cervicovaginal bacteria cause inflammation which in turn increases HIV risk. Profiling the cervicovaginal microbiome, therefore, is instrumental for vaccine development. We show that the microbiome profile captured by cervicovaginal lavage is comparable to samples obtained by vaginal swabs. Thus, lavage may serve as a sampling strategy in NHP vaccine studies.

KW - 16S rRNA

KW - HIV

KW - lavage

KW - swab

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Comparison of sampling methods for profiling cervicovaginal microbiome in rhesus macaques

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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