Comparison of rapid liquid chromatography-electrospray ionization-tandem mass spectrometry methods for determination of glycoalkaloids in transgenic field-grown potatoes

Britta Zywicki, Gareth Catchpole, John Draper, Oliver Fiehn

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Two rapid methods for highly selective detection and quantification of the two major glycoalkaloids in potatoes, α-chaconine and α-solanine, were compared for robustness in high-throughput operations for over 1000 analytical runs using potato tuber samples from field trials. Glycoalkaloids were analyzed using liquid chromatography coupled to tandem mass spectrometry in multiple reaction monitoring mode. An electrospray interface was used in the detection of glycoalkaloids in positive ion mode. Classical reversed phase (RP) and hydrophilic interaction (HILIC) columns were investigated for chromatographic separation, ruggedness, recovery, precision, and accuracy. During the validation procedure both methods proved to be precise and accurate enough in relation to the high degree of endogenous biological variability found for field-grown potato tubers. However, the RP method was found to be more precise, more accurate, and, more importantly, more rugged than the HILIC method for maintaining the analytes' peak shape symmetry in high-throughput operation. When applied to the comparison of six classically bred potato cultivars to six genetically modified (GM) lines engineered to synthesize health beneficial inulins, the glycoalkaloid content in potato peels of all GM lines was found within the range of the six cultivars. We suggest complementing current unbiased metabolomic strategies by validating quantitative analytical methods for important target analytes such as the toxic glycoalkaloids in potato plants.

Original languageEnglish (US)
Pages (from-to)178-186
Number of pages9
JournalAnalytical Biochemistry
Volume336
Issue number2
DOIs
StatePublished - Jan 15 2005
Externally publishedYes

Keywords

  • Fructans
  • High-throughput screening
  • Hydrophilic interaction
  • Liquid chromatography
  • Mass spectrometry
  • Metabolite profiling
  • Method validation
  • Reversed phase
  • Substantial equivalence

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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