Abstract
Objective: To compare agreement between a portable clinical analyzer and laboratory-based bench-top analyzer for analysis of pH, lactate, and glucose concentrations in synovial fluid. Study Design: Prospective experimental study. Animals: Clinically normal horses (n=8); 6 horses euthanatized for reasons unrelated to the study; 11 horses that had synoviocentesis for reasons other than sepsis; 7 horses that had synoviocentesis for evaluation of sepsis; and 2 horses without recorded clinical data. Median age of horses was 8 years (range, 1 day to 24 years). Methods: Supernatant from each synovial fluid sample was analyzed for pH, lactate, and glucose concentrations using an ABL 705 laboratory-based bench-top analyzer and i-STAT portable clinical analyzer. Bland-Altman plots were constructed and concordance analysis performed to determine bias and agreement between the 2 analyzers. Results: There was acceptable agreement between analyzers for lactate and glucose concentrations, with biases of 0.198 mmol/L and 9 mg/dL and concordance correlation coefficients of 0.97 and 0.96 for lactate and glucose, respectively. The agreement between analyzers for pH was not acceptable, with a bias of -0.057 and concordance correlation coefficient of 0.89. Conclusions: This study found that the portable clinical analyzer performed similarly to the bench-top blood gas analyzer for evaluation of lactate and glucose concentrations, but not pH, in synovial fluid.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 811-816 |
| Number of pages | 6 |
| Journal | Veterinary Surgery |
| Volume | 40 |
| Issue number | 7 |
| DOIs | |
| State | Published - Oct 2011 |
| Externally published | Yes |
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ASJC Scopus subject areas
- veterinary(all)
- Medicine(all)
Cite this
Comparison of pH, Lactate, and Glucose Analysis of Equine Synovial Fluid using a Portable Clinical Analyzer with a Bench-Top Blood Gas Analyzer. / Dechant, Julie E; Symm, William A.; Nieto, Jorge.
In: Veterinary Surgery, Vol. 40, No. 7, 10.2011, p. 811-816.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of pH, Lactate, and Glucose Analysis of Equine Synovial Fluid using a Portable Clinical Analyzer with a Bench-Top Blood Gas Analyzer
AU - Dechant, Julie E
AU - Symm, William A.
AU - Nieto, Jorge
PY - 2011/10
Y1 - 2011/10
N2 - Objective: To compare agreement between a portable clinical analyzer and laboratory-based bench-top analyzer for analysis of pH, lactate, and glucose concentrations in synovial fluid. Study Design: Prospective experimental study. Animals: Clinically normal horses (n=8); 6 horses euthanatized for reasons unrelated to the study; 11 horses that had synoviocentesis for reasons other than sepsis; 7 horses that had synoviocentesis for evaluation of sepsis; and 2 horses without recorded clinical data. Median age of horses was 8 years (range, 1 day to 24 years). Methods: Supernatant from each synovial fluid sample was analyzed for pH, lactate, and glucose concentrations using an ABL 705 laboratory-based bench-top analyzer and i-STAT portable clinical analyzer. Bland-Altman plots were constructed and concordance analysis performed to determine bias and agreement between the 2 analyzers. Results: There was acceptable agreement between analyzers for lactate and glucose concentrations, with biases of 0.198 mmol/L and 9 mg/dL and concordance correlation coefficients of 0.97 and 0.96 for lactate and glucose, respectively. The agreement between analyzers for pH was not acceptable, with a bias of -0.057 and concordance correlation coefficient of 0.89. Conclusions: This study found that the portable clinical analyzer performed similarly to the bench-top blood gas analyzer for evaluation of lactate and glucose concentrations, but not pH, in synovial fluid.
AB - Objective: To compare agreement between a portable clinical analyzer and laboratory-based bench-top analyzer for analysis of pH, lactate, and glucose concentrations in synovial fluid. Study Design: Prospective experimental study. Animals: Clinically normal horses (n=8); 6 horses euthanatized for reasons unrelated to the study; 11 horses that had synoviocentesis for reasons other than sepsis; 7 horses that had synoviocentesis for evaluation of sepsis; and 2 horses without recorded clinical data. Median age of horses was 8 years (range, 1 day to 24 years). Methods: Supernatant from each synovial fluid sample was analyzed for pH, lactate, and glucose concentrations using an ABL 705 laboratory-based bench-top analyzer and i-STAT portable clinical analyzer. Bland-Altman plots were constructed and concordance analysis performed to determine bias and agreement between the 2 analyzers. Results: There was acceptable agreement between analyzers for lactate and glucose concentrations, with biases of 0.198 mmol/L and 9 mg/dL and concordance correlation coefficients of 0.97 and 0.96 for lactate and glucose, respectively. The agreement between analyzers for pH was not acceptable, with a bias of -0.057 and concordance correlation coefficient of 0.89. Conclusions: This study found that the portable clinical analyzer performed similarly to the bench-top blood gas analyzer for evaluation of lactate and glucose concentrations, but not pH, in synovial fluid.
UR - http://www.scopus.com/inward/record.url?scp=84864866234&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864866234&partnerID=8YFLogxK
U2 - 10.1111/j.1532-950X.2011.00854.x
DO - 10.1111/j.1532-950X.2011.00854.x
M3 - Article
VL - 40
SP - 811
EP - 816
JO - Veterinary Surgery
JF - Veterinary Surgery
SN - 0161-3499
IS - 7
ER -