Comparison of pH, Lactate, and Glucose Analysis of Equine Synovial Fluid using a Portable Clinical Analyzer with a Bench-Top Blood Gas Analyzer

Julie E Dechant, William A. Symm, Jorge Nieto

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective: To compare agreement between a portable clinical analyzer and laboratory-based bench-top analyzer for analysis of pH, lactate, and glucose concentrations in synovial fluid. Study Design: Prospective experimental study. Animals: Clinically normal horses (n=8); 6 horses euthanatized for reasons unrelated to the study; 11 horses that had synoviocentesis for reasons other than sepsis; 7 horses that had synoviocentesis for evaluation of sepsis; and 2 horses without recorded clinical data. Median age of horses was 8 years (range, 1 day to 24 years). Methods: Supernatant from each synovial fluid sample was analyzed for pH, lactate, and glucose concentrations using an ABL 705 laboratory-based bench-top analyzer and i-STAT portable clinical analyzer. Bland-Altman plots were constructed and concordance analysis performed to determine bias and agreement between the 2 analyzers. Results: There was acceptable agreement between analyzers for lactate and glucose concentrations, with biases of 0.198 mmol/L and 9 mg/dL and concordance correlation coefficients of 0.97 and 0.96 for lactate and glucose, respectively. The agreement between analyzers for pH was not acceptable, with a bias of -0.057 and concordance correlation coefficient of 0.89. Conclusions: This study found that the portable clinical analyzer performed similarly to the bench-top blood gas analyzer for evaluation of lactate and glucose concentrations, but not pH, in synovial fluid.

Original languageEnglish (US)
Pages (from-to)811-816
Number of pages6
JournalVeterinary Surgery
Volume40
Issue number7
DOIs
StatePublished - Oct 2011
Externally publishedYes

Fingerprint

synovial fluid
Synovial Fluid
blood gases
Horses
lactates
Lactic Acid
Gases
horses
Glucose
glucose
Sepsis
Research Design
experimental design
Prospective Studies
animals

ASJC Scopus subject areas

  • veterinary(all)
  • Medicine(all)

Cite this

@article{81831cff2bab42f1bf21774a0407ba81,
title = "Comparison of pH, Lactate, and Glucose Analysis of Equine Synovial Fluid using a Portable Clinical Analyzer with a Bench-Top Blood Gas Analyzer",
abstract = "Objective: To compare agreement between a portable clinical analyzer and laboratory-based bench-top analyzer for analysis of pH, lactate, and glucose concentrations in synovial fluid. Study Design: Prospective experimental study. Animals: Clinically normal horses (n=8); 6 horses euthanatized for reasons unrelated to the study; 11 horses that had synoviocentesis for reasons other than sepsis; 7 horses that had synoviocentesis for evaluation of sepsis; and 2 horses without recorded clinical data. Median age of horses was 8 years (range, 1 day to 24 years). Methods: Supernatant from each synovial fluid sample was analyzed for pH, lactate, and glucose concentrations using an ABL 705 laboratory-based bench-top analyzer and i-STAT portable clinical analyzer. Bland-Altman plots were constructed and concordance analysis performed to determine bias and agreement between the 2 analyzers. Results: There was acceptable agreement between analyzers for lactate and glucose concentrations, with biases of 0.198 mmol/L and 9 mg/dL and concordance correlation coefficients of 0.97 and 0.96 for lactate and glucose, respectively. The agreement between analyzers for pH was not acceptable, with a bias of -0.057 and concordance correlation coefficient of 0.89. Conclusions: This study found that the portable clinical analyzer performed similarly to the bench-top blood gas analyzer for evaluation of lactate and glucose concentrations, but not pH, in synovial fluid.",
author = "Dechant, {Julie E} and Symm, {William A.} and Jorge Nieto",
year = "2011",
month = "10",
doi = "10.1111/j.1532-950X.2011.00854.x",
language = "English (US)",
volume = "40",
pages = "811--816",
journal = "Veterinary Surgery",
issn = "0161-3499",
publisher = "Wiley-Blackwell",
number = "7",

}

TY - JOUR

T1 - Comparison of pH, Lactate, and Glucose Analysis of Equine Synovial Fluid using a Portable Clinical Analyzer with a Bench-Top Blood Gas Analyzer

AU - Dechant, Julie E

AU - Symm, William A.

AU - Nieto, Jorge

PY - 2011/10

Y1 - 2011/10

N2 - Objective: To compare agreement between a portable clinical analyzer and laboratory-based bench-top analyzer for analysis of pH, lactate, and glucose concentrations in synovial fluid. Study Design: Prospective experimental study. Animals: Clinically normal horses (n=8); 6 horses euthanatized for reasons unrelated to the study; 11 horses that had synoviocentesis for reasons other than sepsis; 7 horses that had synoviocentesis for evaluation of sepsis; and 2 horses without recorded clinical data. Median age of horses was 8 years (range, 1 day to 24 years). Methods: Supernatant from each synovial fluid sample was analyzed for pH, lactate, and glucose concentrations using an ABL 705 laboratory-based bench-top analyzer and i-STAT portable clinical analyzer. Bland-Altman plots were constructed and concordance analysis performed to determine bias and agreement between the 2 analyzers. Results: There was acceptable agreement between analyzers for lactate and glucose concentrations, with biases of 0.198 mmol/L and 9 mg/dL and concordance correlation coefficients of 0.97 and 0.96 for lactate and glucose, respectively. The agreement between analyzers for pH was not acceptable, with a bias of -0.057 and concordance correlation coefficient of 0.89. Conclusions: This study found that the portable clinical analyzer performed similarly to the bench-top blood gas analyzer for evaluation of lactate and glucose concentrations, but not pH, in synovial fluid.

AB - Objective: To compare agreement between a portable clinical analyzer and laboratory-based bench-top analyzer for analysis of pH, lactate, and glucose concentrations in synovial fluid. Study Design: Prospective experimental study. Animals: Clinically normal horses (n=8); 6 horses euthanatized for reasons unrelated to the study; 11 horses that had synoviocentesis for reasons other than sepsis; 7 horses that had synoviocentesis for evaluation of sepsis; and 2 horses without recorded clinical data. Median age of horses was 8 years (range, 1 day to 24 years). Methods: Supernatant from each synovial fluid sample was analyzed for pH, lactate, and glucose concentrations using an ABL 705 laboratory-based bench-top analyzer and i-STAT portable clinical analyzer. Bland-Altman plots were constructed and concordance analysis performed to determine bias and agreement between the 2 analyzers. Results: There was acceptable agreement between analyzers for lactate and glucose concentrations, with biases of 0.198 mmol/L and 9 mg/dL and concordance correlation coefficients of 0.97 and 0.96 for lactate and glucose, respectively. The agreement between analyzers for pH was not acceptable, with a bias of -0.057 and concordance correlation coefficient of 0.89. Conclusions: This study found that the portable clinical analyzer performed similarly to the bench-top blood gas analyzer for evaluation of lactate and glucose concentrations, but not pH, in synovial fluid.

UR - http://www.scopus.com/inward/record.url?scp=84864866234&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864866234&partnerID=8YFLogxK

U2 - 10.1111/j.1532-950X.2011.00854.x

DO - 10.1111/j.1532-950X.2011.00854.x

M3 - Article

VL - 40

SP - 811

EP - 816

JO - Veterinary Surgery

JF - Veterinary Surgery

SN - 0161-3499

IS - 7

ER -