Comparison of multiplexed techniques for detection of bacterial and viral proteins

Rupa S. Rao, Steven R. Visuri, Mary T. McBride, Joanna S. Albala, Dennis L Matthews, Matthew A Coleman

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Immobilized antibody microarrays were compared to the Luminex flow cytometry system that utilizes suspensions of polystyrene microbeads covalently coupled with capture antibodies. The two immunoassays were performed for comparison of reproducibility, limits of detection and dynamic range. The Luminex system showed lower limits of detection and increased dynamic range among samples whereas the protein microarrays could be more amenable to miniaturization. Both technologies were capable of sensitive multiplexed detection.

Original languageEnglish (US)
Pages (from-to)736-742
Number of pages7
JournalJournal of Proteome Research
Volume3
Issue number4
DOIs
StatePublished - Jul 2004

Keywords

  • Bead-based assay
  • Detection
  • Immunoassay
  • Luminex
  • Microarray
  • Multiplex assay

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

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