Comparison of mechanical debridement and radiofrequency energy for chondroplasty in an in vivo equine model of partial thickness cartilage injury

R. B. Edwards, Y. Lu, R. K. Uthamanthil, J. J. Bogdanske, P. Muir, K. A. Athanasiou, M. D. Markel

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Objective: The purpose of this study was to develop a long-term model of cartilage injury that could be used to compare the effects of radiofrequency energy (RFE) and mechanical debridement as a treatment. Methods: Partial thickness fibrillation of patellar cartilage was created in 16 mature ponies. Three months after the initial surgery all injured patellae were randomly selected to receive one of the four treatments (n = 8/treatment): (1) control, (2) mechanical debridement with a motorized shaver, (3) TAC-CII RFE probe, and (4) CoVac 50 RFE probe. The ponies were euthanized 22 months after treatment. Macroscopic appearance of the cartilage surface was scored, vital cell staining was used to determine chondrocyte viability and light microscopy was used to grade the morphometric changes within the cartilage. Mechanical properties (aggregate modulus, Poisson's ratio and permeability) also were determined and compared to normal uninjured cartilage. Results: There were no differences in the cartilage surface scores among the treatment groups and control samples (P > 0.05). The maximum depth of cell death and the percentage of dead area in control and mechanical debridement groups were significantly less than those in both RFE groups. There were no significant differences in maximum depth and the percentage of dead area between the two RFE treatment groups. Histologic scores demonstrated better cartilage morphology for the control and mechanical debridement groups than those of RFE groups. However, even with full thickness chondrocyte death, the matrix in the RFE treated sections was still retained and the mechanical properties of the treated cartilage did not differ from the mechanical debridement group. Conclusion: RFE caused greater chondrocyte death and more severe morphological changes compared to untreated degenerative cartilage and mechanical debridement in this model.

Original languageEnglish (US)
Pages (from-to)169-178
Number of pages10
JournalOsteoarthritis and Cartilage
Volume15
Issue number2
DOIs
StatePublished - Feb 2007
Externally publishedYes

Fingerprint

Cartilage
Debridement
Horses
Wounds and Injuries
Chondrocytes
Mechanical properties
Patella
Poisson ratio
Cell death
Surgery
Optical microscopy
Microscopy
Permeability
Cell Death
Staining and Labeling
Light
Control Groups

Keywords

  • Articular cartilage
  • Chondrocyte
  • Confocal laser microscopy
  • Radiofrequency energy
  • Thermal chondroplasty

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Comparison of mechanical debridement and radiofrequency energy for chondroplasty in an in vivo equine model of partial thickness cartilage injury. / Edwards, R. B.; Lu, Y.; Uthamanthil, R. K.; Bogdanske, J. J.; Muir, P.; Athanasiou, K. A.; Markel, M. D.

In: Osteoarthritis and Cartilage, Vol. 15, No. 2, 02.2007, p. 169-178.

Research output: Contribution to journalArticle

Edwards, R. B. ; Lu, Y. ; Uthamanthil, R. K. ; Bogdanske, J. J. ; Muir, P. ; Athanasiou, K. A. ; Markel, M. D. / Comparison of mechanical debridement and radiofrequency energy for chondroplasty in an in vivo equine model of partial thickness cartilage injury. In: Osteoarthritis and Cartilage. 2007 ; Vol. 15, No. 2. pp. 169-178.
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AU - Edwards, R. B.

AU - Lu, Y.

AU - Uthamanthil, R. K.

AU - Bogdanske, J. J.

AU - Muir, P.

AU - Athanasiou, K. A.

AU - Markel, M. D.

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AB - Objective: The purpose of this study was to develop a long-term model of cartilage injury that could be used to compare the effects of radiofrequency energy (RFE) and mechanical debridement as a treatment. Methods: Partial thickness fibrillation of patellar cartilage was created in 16 mature ponies. Three months after the initial surgery all injured patellae were randomly selected to receive one of the four treatments (n = 8/treatment): (1) control, (2) mechanical debridement with a motorized shaver, (3) TAC-CII RFE probe, and (4) CoVac 50 RFE probe. The ponies were euthanized 22 months after treatment. Macroscopic appearance of the cartilage surface was scored, vital cell staining was used to determine chondrocyte viability and light microscopy was used to grade the morphometric changes within the cartilage. Mechanical properties (aggregate modulus, Poisson's ratio and permeability) also were determined and compared to normal uninjured cartilage. Results: There were no differences in the cartilage surface scores among the treatment groups and control samples (P > 0.05). The maximum depth of cell death and the percentage of dead area in control and mechanical debridement groups were significantly less than those in both RFE groups. There were no significant differences in maximum depth and the percentage of dead area between the two RFE treatment groups. Histologic scores demonstrated better cartilage morphology for the control and mechanical debridement groups than those of RFE groups. However, even with full thickness chondrocyte death, the matrix in the RFE treated sections was still retained and the mechanical properties of the treated cartilage did not differ from the mechanical debridement group. Conclusion: RFE caused greater chondrocyte death and more severe morphological changes compared to untreated degenerative cartilage and mechanical debridement in this model.

KW - Articular cartilage

KW - Chondrocyte

KW - Confocal laser microscopy

KW - Radiofrequency energy

KW - Thermal chondroplasty

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