Comparison of intravenous versus intramuscular administration of corticotropin-releasing hormone in healthy cats

K. C. Eiler, D. S. Bruyette, E. N. Behrend, R. J. Kemppainen, Philip H Kass

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Because of the lack of a current validated assay for feline endogenous adrenocorticotropic hormone (ACTH) in response to administration of currently available ovine corticotropin-releasing hormone (oCRH) preparations, a complete evaluation of the hypothalamic-pituitary-adrenal axis in cats has not been possible. Objective: This study was undertaken to (1) determine the pituitary (ACTH) and adrenal (cortisol) response to both IV and IM administration of a currently available oCRH product in healthy cats, and (2) validate an endogenous ACTH assay for use in cats. Animals: Seventeen healthy cats receiving oCRH (n = 11) or placebo (n = 6). Methods: Prospective, randomized, placebo-controlled study. oCRH at 1 μg/kg or placebo was given either IM or IV. Endogenous cortisol and ACTH concentrations were evaluated after the injection. A comparison of IM versus IV and placebo versus treatment was made. Results: The DiaSorin immunoradiometric assay (IRMA) assay for ACTH performed well, showing both parallelism and acceptable intra- and interassay coefficients of variation. There was a significant difference between groups (P = .025) and a significant difference between times (P = .025) when endogenous ACTH concentrations were compared after oCRH IV or IM. No significant differences were observed in cortisol concentrations comparing IV to IM oCRH. Conclusions: IM administration of oCRH results in significantly greater ACTH concentrations but not cortisol concentrations when compared with IV administration. Samples should be drawn before and at 60 minutes after the injection. The Diasorin IRMA is valid for feline endogenous ACTH measurements.

Original languageEnglish (US)
Pages (from-to)516-521
Number of pages6
JournalJournal of Veterinary Internal Medicine
Volume27
Issue number3
DOIs
StatePublished - May 2013

Fingerprint

corticotropin-releasing hormone
Corticotropin-Releasing Hormone
corticotropin
intramuscular injection
Adrenocorticotropic Hormone
Cats
Sheep
cats
sheep
placebos
cortisol
Hydrocortisone
Placebos
assays
Immunoradiometric Assay
Felidae
injection
pituitary hormones
Injections
Pituitary Hormones

Keywords

  • ACTH
  • Adrenal
  • Cortisol
  • Pituitary

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Comparison of intravenous versus intramuscular administration of corticotropin-releasing hormone in healthy cats. / Eiler, K. C.; Bruyette, D. S.; Behrend, E. N.; Kemppainen, R. J.; Kass, Philip H.

In: Journal of Veterinary Internal Medicine, Vol. 27, No. 3, 05.2013, p. 516-521.

Research output: Contribution to journalArticle

Eiler, K. C. ; Bruyette, D. S. ; Behrend, E. N. ; Kemppainen, R. J. ; Kass, Philip H. / Comparison of intravenous versus intramuscular administration of corticotropin-releasing hormone in healthy cats. In: Journal of Veterinary Internal Medicine. 2013 ; Vol. 27, No. 3. pp. 516-521.
@article{a934b21c30d04cd4beef738d0ec26223,
title = "Comparison of intravenous versus intramuscular administration of corticotropin-releasing hormone in healthy cats",
abstract = "Background: Because of the lack of a current validated assay for feline endogenous adrenocorticotropic hormone (ACTH) in response to administration of currently available ovine corticotropin-releasing hormone (oCRH) preparations, a complete evaluation of the hypothalamic-pituitary-adrenal axis in cats has not been possible. Objective: This study was undertaken to (1) determine the pituitary (ACTH) and adrenal (cortisol) response to both IV and IM administration of a currently available oCRH product in healthy cats, and (2) validate an endogenous ACTH assay for use in cats. Animals: Seventeen healthy cats receiving oCRH (n = 11) or placebo (n = 6). Methods: Prospective, randomized, placebo-controlled study. oCRH at 1 μg/kg or placebo was given either IM or IV. Endogenous cortisol and ACTH concentrations were evaluated after the injection. A comparison of IM versus IV and placebo versus treatment was made. Results: The DiaSorin immunoradiometric assay (IRMA) assay for ACTH performed well, showing both parallelism and acceptable intra- and interassay coefficients of variation. There was a significant difference between groups (P = .025) and a significant difference between times (P = .025) when endogenous ACTH concentrations were compared after oCRH IV or IM. No significant differences were observed in cortisol concentrations comparing IV to IM oCRH. Conclusions: IM administration of oCRH results in significantly greater ACTH concentrations but not cortisol concentrations when compared with IV administration. Samples should be drawn before and at 60 minutes after the injection. The Diasorin IRMA is valid for feline endogenous ACTH measurements.",
keywords = "ACTH, Adrenal, Cortisol, Pituitary",
author = "Eiler, {K. C.} and Bruyette, {D. S.} and Behrend, {E. N.} and Kemppainen, {R. J.} and Kass, {Philip H}",
year = "2013",
month = "5",
doi = "10.1111/jvim.12054",
language = "English (US)",
volume = "27",
pages = "516--521",
journal = "Journal of Veterinary Internal Medicine",
issn = "0891-6640",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Comparison of intravenous versus intramuscular administration of corticotropin-releasing hormone in healthy cats

AU - Eiler, K. C.

AU - Bruyette, D. S.

AU - Behrend, E. N.

AU - Kemppainen, R. J.

AU - Kass, Philip H

PY - 2013/5

Y1 - 2013/5

N2 - Background: Because of the lack of a current validated assay for feline endogenous adrenocorticotropic hormone (ACTH) in response to administration of currently available ovine corticotropin-releasing hormone (oCRH) preparations, a complete evaluation of the hypothalamic-pituitary-adrenal axis in cats has not been possible. Objective: This study was undertaken to (1) determine the pituitary (ACTH) and adrenal (cortisol) response to both IV and IM administration of a currently available oCRH product in healthy cats, and (2) validate an endogenous ACTH assay for use in cats. Animals: Seventeen healthy cats receiving oCRH (n = 11) or placebo (n = 6). Methods: Prospective, randomized, placebo-controlled study. oCRH at 1 μg/kg or placebo was given either IM or IV. Endogenous cortisol and ACTH concentrations were evaluated after the injection. A comparison of IM versus IV and placebo versus treatment was made. Results: The DiaSorin immunoradiometric assay (IRMA) assay for ACTH performed well, showing both parallelism and acceptable intra- and interassay coefficients of variation. There was a significant difference between groups (P = .025) and a significant difference between times (P = .025) when endogenous ACTH concentrations were compared after oCRH IV or IM. No significant differences were observed in cortisol concentrations comparing IV to IM oCRH. Conclusions: IM administration of oCRH results in significantly greater ACTH concentrations but not cortisol concentrations when compared with IV administration. Samples should be drawn before and at 60 minutes after the injection. The Diasorin IRMA is valid for feline endogenous ACTH measurements.

AB - Background: Because of the lack of a current validated assay for feline endogenous adrenocorticotropic hormone (ACTH) in response to administration of currently available ovine corticotropin-releasing hormone (oCRH) preparations, a complete evaluation of the hypothalamic-pituitary-adrenal axis in cats has not been possible. Objective: This study was undertaken to (1) determine the pituitary (ACTH) and adrenal (cortisol) response to both IV and IM administration of a currently available oCRH product in healthy cats, and (2) validate an endogenous ACTH assay for use in cats. Animals: Seventeen healthy cats receiving oCRH (n = 11) or placebo (n = 6). Methods: Prospective, randomized, placebo-controlled study. oCRH at 1 μg/kg or placebo was given either IM or IV. Endogenous cortisol and ACTH concentrations were evaluated after the injection. A comparison of IM versus IV and placebo versus treatment was made. Results: The DiaSorin immunoradiometric assay (IRMA) assay for ACTH performed well, showing both parallelism and acceptable intra- and interassay coefficients of variation. There was a significant difference between groups (P = .025) and a significant difference between times (P = .025) when endogenous ACTH concentrations were compared after oCRH IV or IM. No significant differences were observed in cortisol concentrations comparing IV to IM oCRH. Conclusions: IM administration of oCRH results in significantly greater ACTH concentrations but not cortisol concentrations when compared with IV administration. Samples should be drawn before and at 60 minutes after the injection. The Diasorin IRMA is valid for feline endogenous ACTH measurements.

KW - ACTH

KW - Adrenal

KW - Cortisol

KW - Pituitary

UR - http://www.scopus.com/inward/record.url?scp=84877650732&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877650732&partnerID=8YFLogxK

U2 - 10.1111/jvim.12054

DO - 10.1111/jvim.12054

M3 - Article

C2 - 23480784

AN - SCOPUS:84877650732

VL - 27

SP - 516

EP - 521

JO - Journal of Veterinary Internal Medicine

JF - Journal of Veterinary Internal Medicine

SN - 0891-6640

IS - 3

ER -