Comparison of histologic and flow cytometric evaluation of cyclophosphamide-induced testicular damage

Susan D. Goldberg, Arline D. Deitch, Maria Schevchuck, Harris M. Nagler, Ralph W deVere White

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This study evaluates the ability of DNA histograms obtained by flow cytometry to detect and quantify reversible alterations in spermatogenesis induced by cyclophosphamide, a known inhibitor of spermatogenesis. Evaluation of per cent of cells in each of the haploid (lc), diploid (2c), and tetraploid peaks (4c) as determined by flow cytometry in treated and control Balb/C mice over a six-week period, and comparison with routine histologic evaluation have led us to conclude that DNA histogram evaluation is a rapid and accurate means of identifying testicular damage and recovery. This technique may be useful in sequential monitoring of the effects of malignancy and/or treatments applied on spermatogenesis in young men.

Original languageEnglish (US)
Pages (from-to)472-475
Number of pages4
JournalUrology
Volume24
Issue number5
DOIs
StatePublished - 1984
Externally publishedYes

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Spermatogenesis
Cyclophosphamide
Flow Cytometry
Tetraploidy
Haploidy
DNA
Diploidy
Neoplasms
Therapeutics

ASJC Scopus subject areas

  • Urology

Cite this

Comparison of histologic and flow cytometric evaluation of cyclophosphamide-induced testicular damage. / Goldberg, Susan D.; Deitch, Arline D.; Schevchuck, Maria; Nagler, Harris M.; deVere White, Ralph W.

In: Urology, Vol. 24, No. 5, 1984, p. 472-475.

Research output: Contribution to journalArticle

Goldberg, Susan D. ; Deitch, Arline D. ; Schevchuck, Maria ; Nagler, Harris M. ; deVere White, Ralph W. / Comparison of histologic and flow cytometric evaluation of cyclophosphamide-induced testicular damage. In: Urology. 1984 ; Vol. 24, No. 5. pp. 472-475.
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