Comparison of Helicobacter pylori virulence gene expression in vitro and in the rhesus macaque

Jenni K. Boonjakuakul, Don R. Canfield, Jay V Solnick

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

We used a quantitative real-time reverse transcriptase PCR assay to measure the transcript abundance of 46 known and putative Helicobacter pylori virulence genes, including 24 genes on the Cag pathogenicity island. The expression profile of H. pylori cells grown in vitro was also compared to expression in vivo after experimental infection of rhesus macaques. Transcript abundance in vitro (mid-log phase) ranged from about 0.004 (feoB and hpaA) to 20 (ureAB, nap A, and cag25) copies/cell. Expression of most genes was repressed during the transition from logarithmic- to stationary-phase growth, but several well-characterized H. pylori virulence genes (katA, nap A, vacA, and cagA) were induced. Comparison of results in the rhesus macaque with similar data from humans showed a strong correlation (r = 0.89). The relative in vivo expression in the rhesus monkey was highly correlated with in vitro expression during mid-log (r = 0.89)- and stationary (r = 0.88)-phase growth. Transcript abundance was on average three- to fourfold reduced in vivo compared to in vitro during mid-log phase. However, when compared to stationary phase, increased expression in vivo was observed for 6 of 7 genes on a contiguous portion of the pathogenicity island, several of which are thought to encode the H. pylori type IV structural pilus and its accessory proteins. These results suggest the possibility that some genes encoding the H. pylori type IV structural pilus and accessory proteins may form an operon that is induced during growth in vivo.

Original languageEnglish (US)
Pages (from-to)4895-4904
Number of pages10
JournalInfection and Immunity
Volume73
Issue number8
DOIs
StatePublished - Aug 2005

ASJC Scopus subject areas

  • Immunology

Fingerprint

Dive into the research topics of 'Comparison of Helicobacter pylori virulence gene expression in vitro and in the rhesus macaque'. Together they form a unique fingerprint.

Cite this