Comparison of freeze-thaw cycles for nucleic acid extraction and molecular detection of Cryptosporidium parvum and Toxoplasma gondii oocysts in environmental matrices

Anna J.W. Manore, Sherilee L. Harper, Beatriz Aguilar, J. S. Weese, Karen Shapiro

Research output: Contribution to journalArticle

Abstract

Freeze-thaw DNA extraction methods and PCR primers were compared to optimize detection of Cryptosporidium parvum and Toxoplasma gondii oocysts in different matrices. Increasing FT cycles did not increase parasite DNA detection, and primers targeting the 18S ssrRNA gene yielded the most sensitive detection of C. parvum oocysts.

LanguageEnglish (US)
Pages1-4
Number of pages4
JournalJournal of Microbiological Methods
Volume156
DOIs
StatePublished - Jan 1 2019

Fingerprint

Cryptosporidium parvum
Oocysts
Toxoplasma
Nucleic Acids
DNA Primers
Parasites
Polymerase Chain Reaction
DNA
Genes

Keywords

  • Cryptosporidium parvum
  • Detection
  • DNA extraction
  • Freeze-thaw
  • PCR
  • Toxoplasma gondii

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Cite this

Comparison of freeze-thaw cycles for nucleic acid extraction and molecular detection of Cryptosporidium parvum and Toxoplasma gondii oocysts in environmental matrices. / Manore, Anna J.W.; Harper, Sherilee L.; Aguilar, Beatriz; Weese, J. S.; Shapiro, Karen.

In: Journal of Microbiological Methods, Vol. 156, 01.01.2019, p. 1-4.

Research output: Contribution to journalArticle

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