Comparison of free serum oxylipin concentrations in hyper- vs. normolipidemic men

Jan Philipp Schuchardt, Simone Schmidt, Gaby Kressel, Hua Dong, Ina Willenberg, Bruce D. Hammock, Andreas Hahn, Nils Helge Schebb

Research output: Contribution to journalArticlepeer-review

63 Scopus citations


Oxylipins, the oxidation products of unsaturated fatty acids (FA), are potent endogenous mediators being involved in the regulation of various biological processes such as inflammation, pain and blood coagulation. Compared to oxylipins derived from arachidonic acid (AA) by cyclooxygenase action, i.e. prostanoides, only limited information is available about the endogenous levels of hydroxy-, epoxy- and dihydroxy-FA of linoleic acid (LA), AA, α-linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in humans. Particularly, it is unknown how metabolic disorders affect endogenous oxylipin levels in humans. Therefore, in the present study we compared the serum concentrations of 44 oxylipins in 20 normolipidemic with 20 hyperlipidemic (total cholesterol >200. mg/dl; LDL-C>130. mg/dl; TG>150. mg/dl) men (age 29-51. y). The serum concentration varied strongly among subjects. For most hydroxy-, epoxy- and dihydroxy-FA the concentrations were comparable to those in plasma reported in earlier studies. Despite the significant change in blood lipid levels the hyperlipidemic group showed only minor differences in oxylipin levels. The hyperlipidemic subjects had a slightly higher serum concentration of 8,9-DiHETrE, 5-HEPE, 10,11-DiHDPE, and a lower concentration of 12,13-DiHOME, 12-HETE, 9,10-DiHODE, and 12,13-DiHODE compared to normolipidemic subjects. Overall the hydroxy-, epoxy- and dihydroxy-FA levels were not changed suggesting that mild combined hyperlipidemia has no apparent effect on the concentration of circulating oxylipins. By contrast, serum levels of several hydroxy-, epoxy-, and dihydroxy-FA are dependent on the individual status of the parent FA. Particularly, a strong correlation between the EPA content in the erythrocyte membrane and the serum concentration of EPA derived oxylipins was observed. Given that the synthesis of EPA from other n-3 FA in humans is low; this suggests that oxylipin levels can be directly influenced by the diet.

Original languageEnglish (US)
Pages (from-to)19-29
Number of pages11
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Issue number1
StatePublished - Jul 2013


  • Arachidonic acid
  • Eicosanoids
  • Eicosapentaenoic acid
  • Hyperlipidemia
  • Omega-3 fatty acids
  • PUFA

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry


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