Comparison of five different RNA sources to examine the lactating bovine mammary gland transcriptome using RNA-Sequencing

Angela Cánovas, Gonzalo Rincón, Claudia Bevilacqua, Alma Islas-Trejo, Pauline Brenaut, Russell C. Hovey, Marion Boutinaud, Caroline Morgenthaler, Monica K. Vanklompenberg, Patrice Martin, Juan F. Medrano

Research output: Contribution to journalArticle

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Abstract

The objective of this study was to examine five different sources of RNA, namely mammary gland tissue (MGT), milk somatic cells (SC), laser microdissected mammary epithelial cells (LCMEC), milk fat globules (MFG) and antibody-captured milk mammary epithelial cells (mMEC) to analyze the bovine mammary gland transcriptome using RNA-Sequencing. Our results provide a comparison between different sampling methods (invasive and non-invasive) to define the transcriptome of mammary gland tissue and milk cells. This information will be of value to investigators in choosing the most appropriate sampling method for different research applications to study specific physiological states during lactation. One of the simplest procedures to study the transcriptome associated with milk appears to be the isolation of total RNA directly from SC or MFG released into milk during lactation. Our results indicate that the SC and MFG transcriptome are representative of MGT and LCMEC and can be used as effective and alternative samples to study mammary gland expression without the need to perform a tissue biopsy.

Original languageEnglish (US)
Article number5297
JournalScientific Reports
Volume4
DOIs
StatePublished - Jul 8 2014

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RNA Sequence Analysis
Human Mammary Glands
Transcriptome
RNA
Milk
Breast
Epithelial Cells
Lactation
Lasers
Human Milk
Research Personnel
Biopsy
Antibodies
Research
milk fat globule

ASJC Scopus subject areas

  • General

Cite this

Cánovas, A., Rincón, G., Bevilacqua, C., Islas-Trejo, A., Brenaut, P., Hovey, R. C., ... Medrano, J. F. (2014). Comparison of five different RNA sources to examine the lactating bovine mammary gland transcriptome using RNA-Sequencing. Scientific Reports, 4, [5297]. https://doi.org/10.1038/srep05297

Comparison of five different RNA sources to examine the lactating bovine mammary gland transcriptome using RNA-Sequencing. / Cánovas, Angela; Rincón, Gonzalo; Bevilacqua, Claudia; Islas-Trejo, Alma; Brenaut, Pauline; Hovey, Russell C.; Boutinaud, Marion; Morgenthaler, Caroline; Vanklompenberg, Monica K.; Martin, Patrice; Medrano, Juan F.

In: Scientific Reports, Vol. 4, 5297, 08.07.2014.

Research output: Contribution to journalArticle

Cánovas, A, Rincón, G, Bevilacqua, C, Islas-Trejo, A, Brenaut, P, Hovey, RC, Boutinaud, M, Morgenthaler, C, Vanklompenberg, MK, Martin, P & Medrano, JF 2014, 'Comparison of five different RNA sources to examine the lactating bovine mammary gland transcriptome using RNA-Sequencing', Scientific Reports, vol. 4, 5297. https://doi.org/10.1038/srep05297
Cánovas, Angela ; Rincón, Gonzalo ; Bevilacqua, Claudia ; Islas-Trejo, Alma ; Brenaut, Pauline ; Hovey, Russell C. ; Boutinaud, Marion ; Morgenthaler, Caroline ; Vanklompenberg, Monica K. ; Martin, Patrice ; Medrano, Juan F. / Comparison of five different RNA sources to examine the lactating bovine mammary gland transcriptome using RNA-Sequencing. In: Scientific Reports. 2014 ; Vol. 4.
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