Comparison of Edwardsiella ictaluri isolates from different hosts and geographic origins

M. J. Griffin, S. R. Reichley, T. E. Greenway, S. M. Quiniou, C. Ware, D. X. Gao, P. S. Gaunt, R. P.E. Yanong, D. B. Pouder, J. P. Hawke, Esteban Soto Martinez

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The intraspecific variability of E. ictaluri isolates from different origins was investigated. Isolates were recovered from farm-raised catfish (Ictalurus punctatus) in Mississippi, USA, tilapia (Oreochromis niloticus) cultured in the Western Hemisphere and zebrafish (Danio rerio) propagated in Florida, USA. These isolates were phenotypically homologous and antimicrobial profiles were largely similar. Genetically, isolates possessed differences that could be exploited by repetitive-sequence-mediated PCR and gyrB sequence, which identified three distinct E. ictaluri genotypes: one associated with catfish, one from tilapia and a third from zebrafish. Plasmid profiles were also group specific and correlated with rep-PCR and gyrB sequences. The catfish isolates possessed profiles typical of those described for E. ictaluri isolates; however, plasmids from the zebrafish and tilapia isolates differed in both composition and arrangement. Furthermore, some zebrafish and tilapia isolates were PCR negative for several E. ictaluri virulence factors. Isolates were serologically heterogenous, as serum from a channel catfish exposed to a catfish isolate had reduced antibody activity to tilapia and zebrafish isolates. This work identifies three genetically distinct strains of E. ictaluri from different origins using rep-PCR, 16S, gyrB and plasmid sequencing, in addition to antimicrobial and serological profiling.

Original languageEnglish (US)
Pages (from-to)947-969
Number of pages23
JournalJournal of Fish Diseases
Volume39
Issue number8
DOIs
StatePublished - Aug 1 2016
Externally publishedYes

Fingerprint

Edwardsiella ictaluri
Tilapia
Zebrafish
plasmid
Danio rerio
provenance
Catfishes
tilapia (common name)
catfish
Ictaluridae
Polymerase Chain Reaction
plasmids
Plasmids
Ictalurus punctatus
virulence
antibody
serum
genotype
anti-infective agents
farm

Keywords

  • catfish
  • Edwardsiella
  • tilapia
  • zebrafish

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary (miscalleneous)

Cite this

Griffin, M. J., Reichley, S. R., Greenway, T. E., Quiniou, S. M., Ware, C., Gao, D. X., ... Soto Martinez, E. (2016). Comparison of Edwardsiella ictaluri isolates from different hosts and geographic origins. Journal of Fish Diseases, 39(8), 947-969. https://doi.org/10.1111/jfd.12431

Comparison of Edwardsiella ictaluri isolates from different hosts and geographic origins. / Griffin, M. J.; Reichley, S. R.; Greenway, T. E.; Quiniou, S. M.; Ware, C.; Gao, D. X.; Gaunt, P. S.; Yanong, R. P.E.; Pouder, D. B.; Hawke, J. P.; Soto Martinez, Esteban.

In: Journal of Fish Diseases, Vol. 39, No. 8, 01.08.2016, p. 947-969.

Research output: Contribution to journalArticle

Griffin, MJ, Reichley, SR, Greenway, TE, Quiniou, SM, Ware, C, Gao, DX, Gaunt, PS, Yanong, RPE, Pouder, DB, Hawke, JP & Soto Martinez, E 2016, 'Comparison of Edwardsiella ictaluri isolates from different hosts and geographic origins', Journal of Fish Diseases, vol. 39, no. 8, pp. 947-969. https://doi.org/10.1111/jfd.12431
Griffin MJ, Reichley SR, Greenway TE, Quiniou SM, Ware C, Gao DX et al. Comparison of Edwardsiella ictaluri isolates from different hosts and geographic origins. Journal of Fish Diseases. 2016 Aug 1;39(8):947-969. https://doi.org/10.1111/jfd.12431
Griffin, M. J. ; Reichley, S. R. ; Greenway, T. E. ; Quiniou, S. M. ; Ware, C. ; Gao, D. X. ; Gaunt, P. S. ; Yanong, R. P.E. ; Pouder, D. B. ; Hawke, J. P. ; Soto Martinez, Esteban. / Comparison of Edwardsiella ictaluri isolates from different hosts and geographic origins. In: Journal of Fish Diseases. 2016 ; Vol. 39, No. 8. pp. 947-969.
@article{aba372cd611642118b445ba6fe61d02e,
title = "Comparison of Edwardsiella ictaluri isolates from different hosts and geographic origins",
abstract = "The intraspecific variability of E. ictaluri isolates from different origins was investigated. Isolates were recovered from farm-raised catfish (Ictalurus punctatus) in Mississippi, USA, tilapia (Oreochromis niloticus) cultured in the Western Hemisphere and zebrafish (Danio rerio) propagated in Florida, USA. These isolates were phenotypically homologous and antimicrobial profiles were largely similar. Genetically, isolates possessed differences that could be exploited by repetitive-sequence-mediated PCR and gyrB sequence, which identified three distinct E. ictaluri genotypes: one associated with catfish, one from tilapia and a third from zebrafish. Plasmid profiles were also group specific and correlated with rep-PCR and gyrB sequences. The catfish isolates possessed profiles typical of those described for E. ictaluri isolates; however, plasmids from the zebrafish and tilapia isolates differed in both composition and arrangement. Furthermore, some zebrafish and tilapia isolates were PCR negative for several E. ictaluri virulence factors. Isolates were serologically heterogenous, as serum from a channel catfish exposed to a catfish isolate had reduced antibody activity to tilapia and zebrafish isolates. This work identifies three genetically distinct strains of E. ictaluri from different origins using rep-PCR, 16S, gyrB and plasmid sequencing, in addition to antimicrobial and serological profiling.",
keywords = "catfish, Edwardsiella, tilapia, zebrafish",
author = "Griffin, {M. J.} and Reichley, {S. R.} and Greenway, {T. E.} and Quiniou, {S. M.} and C. Ware and Gao, {D. X.} and Gaunt, {P. S.} and Yanong, {R. P.E.} and Pouder, {D. B.} and Hawke, {J. P.} and {Soto Martinez}, Esteban",
year = "2016",
month = "8",
day = "1",
doi = "10.1111/jfd.12431",
language = "English (US)",
volume = "39",
pages = "947--969",
journal = "Journal of Fish Diseases",
issn = "0140-7775",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - Comparison of Edwardsiella ictaluri isolates from different hosts and geographic origins

AU - Griffin, M. J.

AU - Reichley, S. R.

AU - Greenway, T. E.

AU - Quiniou, S. M.

AU - Ware, C.

AU - Gao, D. X.

AU - Gaunt, P. S.

AU - Yanong, R. P.E.

AU - Pouder, D. B.

AU - Hawke, J. P.

AU - Soto Martinez, Esteban

PY - 2016/8/1

Y1 - 2016/8/1

N2 - The intraspecific variability of E. ictaluri isolates from different origins was investigated. Isolates were recovered from farm-raised catfish (Ictalurus punctatus) in Mississippi, USA, tilapia (Oreochromis niloticus) cultured in the Western Hemisphere and zebrafish (Danio rerio) propagated in Florida, USA. These isolates were phenotypically homologous and antimicrobial profiles were largely similar. Genetically, isolates possessed differences that could be exploited by repetitive-sequence-mediated PCR and gyrB sequence, which identified three distinct E. ictaluri genotypes: one associated with catfish, one from tilapia and a third from zebrafish. Plasmid profiles were also group specific and correlated with rep-PCR and gyrB sequences. The catfish isolates possessed profiles typical of those described for E. ictaluri isolates; however, plasmids from the zebrafish and tilapia isolates differed in both composition and arrangement. Furthermore, some zebrafish and tilapia isolates were PCR negative for several E. ictaluri virulence factors. Isolates were serologically heterogenous, as serum from a channel catfish exposed to a catfish isolate had reduced antibody activity to tilapia and zebrafish isolates. This work identifies three genetically distinct strains of E. ictaluri from different origins using rep-PCR, 16S, gyrB and plasmid sequencing, in addition to antimicrobial and serological profiling.

AB - The intraspecific variability of E. ictaluri isolates from different origins was investigated. Isolates were recovered from farm-raised catfish (Ictalurus punctatus) in Mississippi, USA, tilapia (Oreochromis niloticus) cultured in the Western Hemisphere and zebrafish (Danio rerio) propagated in Florida, USA. These isolates were phenotypically homologous and antimicrobial profiles were largely similar. Genetically, isolates possessed differences that could be exploited by repetitive-sequence-mediated PCR and gyrB sequence, which identified three distinct E. ictaluri genotypes: one associated with catfish, one from tilapia and a third from zebrafish. Plasmid profiles were also group specific and correlated with rep-PCR and gyrB sequences. The catfish isolates possessed profiles typical of those described for E. ictaluri isolates; however, plasmids from the zebrafish and tilapia isolates differed in both composition and arrangement. Furthermore, some zebrafish and tilapia isolates were PCR negative for several E. ictaluri virulence factors. Isolates were serologically heterogenous, as serum from a channel catfish exposed to a catfish isolate had reduced antibody activity to tilapia and zebrafish isolates. This work identifies three genetically distinct strains of E. ictaluri from different origins using rep-PCR, 16S, gyrB and plasmid sequencing, in addition to antimicrobial and serological profiling.

KW - catfish

KW - Edwardsiella

KW - tilapia

KW - zebrafish

UR - http://www.scopus.com/inward/record.url?scp=84949994599&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949994599&partnerID=8YFLogxK

U2 - 10.1111/jfd.12431

DO - 10.1111/jfd.12431

M3 - Article

VL - 39

SP - 947

EP - 969

JO - Journal of Fish Diseases

JF - Journal of Fish Diseases

SN - 0140-7775

IS - 8

ER -