Comparison of different regimens of human gonadotropins for superovulation of rhesus monkeys: Ovulatory response and subsequent luteal function

Catherine A. Vandevoort, William L. Baughman, Richard L. Stouffer

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

This study was designed to identify suitable treatment regimens of human gonadotropin for superovulation of rhesus monkeys. At menses, female monkeys were given one of three regimens: Plan A [days 1 to 6, 60 IU human follicle-stimulating hormone (hFSH); days 7 to 9, 60 IU hFSH/60 IU human luteinizing hormone (hLH)], Plan B [days 1 to 3, 75 IU FSH/20 IU LH; days 4 to 6, 60 IU FSH/20 IU LH; days 7 to 9, 45 IU FSH/45 IU LH], or Plan C [days 1 to 9, 60 IU FSH/60 IU LH]. On day 10, human chorionic gonadotropin (hCG; 1000 IU) was administered. Serum estrogen levels peaked on the day of hCG treatment (day 10) in Plans A and C but earlier (day 8) in Plan B. An oviduct lavage recovered 1 to 3 oocytes in Plan B but 3 to 13 oocytes in the other treatment groups. Peak progesterone levels in the luteal phase were greater (P<0.05) in animals receiving Plan A or C than Plan B. Regardless of treatment group, progesterone levels declined abruptly 7 days after ovulation induction; the length of the luteal phase in all groups was significantly less than that of normal menstrual cycles. We conclude that regimens of hFSH and hLH (i.e., Plans A and C), followed by hCG, reliably superovulate rhesus monkeys. However, the premature decline in luteal function around the typical time of implantation may compromise pregnancy initiation and maintenance.

Original languageEnglish (US)
Pages (from-to)85-91
Number of pages7
JournalJournal of in Vitro Fertilization and Embryo Transfer
Volume6
Issue number2
DOIs
StatePublished - Apr 1989
Externally publishedYes

Fingerprint

Superovulation
Levonorgestrel
Corpus Luteum
Human Follicle Stimulating Hormone
Macaca mulatta
Gonadotropins
Luteal Phase
Luteinizing Hormone
Oocytes
Progesterone
Pregnancy Maintenance
Menstruation
Oviducts
Ovulation Induction
Therapeutic Irrigation
Chorionic Gonadotropin
Therapeutics
Menstrual Cycle
Haplorhini
Estrogens

Keywords

  • corpus luteum
  • gonadotropins
  • ovulation
  • rhesus monkeys
  • steroids

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Embryology
  • Developmental Biology

Cite this

Comparison of different regimens of human gonadotropins for superovulation of rhesus monkeys : Ovulatory response and subsequent luteal function. / Vandevoort, Catherine A.; Baughman, William L.; Stouffer, Richard L.

In: Journal of in Vitro Fertilization and Embryo Transfer, Vol. 6, No. 2, 04.1989, p. 85-91.

Research output: Contribution to journalArticle

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