Comparison of different primers for rapid detection of Salmonella using the polymerase chain reaction

C. M. Gooding, Prabhakara V Choudary

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28 Scopus citations

Abstract

Salmonella is the leading cause of food-borne diarrhoeas in the US. In recent years polymerase chain reaction (PCR) has become the method of choice for rapid and sensitive detection of Salmonellae in contaminated foods. As a result, several different primer sets have been reported for use in PCR-based assay systems. In order to identify an optimal primer set from among the wide range of primers reported in the literature, we synthesized five different pairs and evaluated their relative performance in PCR under uniform assay conditions using a common panel of the target (Salmonella) and non-target (non-Salmonella) bacterial strains. Of the five sets of primers tested, the one designed on the basis of a 199 bp repeat sequence of S. weltevreden gave optimal results with most bacterial strains examined.

Original languageEnglish (US)
Pages (from-to)341-347
Number of pages7
JournalMolecular and Cellular Probes
Volume13
Issue number5
DOIs
StatePublished - Oct 1999

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ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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