Comparison of diagnostic methods to detect piroplasms in asymptomatic cattle

R. Galuppi, C. Bonoli, S. Aureli, R. Cassini, F. Marcer, Janet E Foley, M. P. Tampieri

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study was carried out to compare different diagnostic techniques to reveal the presence of piroplasms in asymptomatic cattle kept at pasture. Nineteen blood samples were collected from animals of two different areas of Emilia Romagna Region of Italy and processed for microscopic observation, PCR, serological test (IFAT) for Babesia bovis and Babesia bigemina antibodies and in vitro cultivation. The cultures were performed on both bovine and ovine erythrocytes. Seventeen blood smears (89%) were positive for piroplasms, while PCR was positive on 18 samples (95%). DNA sequencing of 18S rRNA identified the piroplasms as Theileria spp. In vitro cultures were successful for 6 samples (32%) cultured on bovine blood and subsequent identified these as Babesia major by PCR. On IFAT analyses of 16 samples, 36.8% resulted positive for B. bovis and 31.6% positive for B. bigemina.These results show, in the same animals, the co-infection with Babesia spp. and Theileria spp.; the detection of B. major was possible only using the in vitro cultures.

Original languageEnglish (US)
Pages (from-to)364-368
Number of pages5
JournalVeterinary Parasitology
Volume183
Issue number3-4
DOIs
StatePublished - Feb 10 2012

Fingerprint

Piroplasmida
Babesia
Babesia bovis
Theileria
diagnostic techniques
Babesia bigemina
Polymerase Chain Reaction
cattle
in vitro culture
blood
Italy
Serologic Tests
Coinfection
DNA Sequence Analysis
sampling
Sheep
immunologic techniques
Erythrocytes
mixed infection
animals

Keywords

  • Babesia spp.
  • Blood cultures
  • Blood smear
  • PCR
  • Serology
  • Theileria spp.

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)

Cite this

Galuppi, R., Bonoli, C., Aureli, S., Cassini, R., Marcer, F., Foley, J. E., & Tampieri, M. P. (2012). Comparison of diagnostic methods to detect piroplasms in asymptomatic cattle. Veterinary Parasitology, 183(3-4), 364-368. https://doi.org/10.1016/j.vetpar.2011.07.037

Comparison of diagnostic methods to detect piroplasms in asymptomatic cattle. / Galuppi, R.; Bonoli, C.; Aureli, S.; Cassini, R.; Marcer, F.; Foley, Janet E; Tampieri, M. P.

In: Veterinary Parasitology, Vol. 183, No. 3-4, 10.02.2012, p. 364-368.

Research output: Contribution to journalArticle

Galuppi, R, Bonoli, C, Aureli, S, Cassini, R, Marcer, F, Foley, JE & Tampieri, MP 2012, 'Comparison of diagnostic methods to detect piroplasms in asymptomatic cattle', Veterinary Parasitology, vol. 183, no. 3-4, pp. 364-368. https://doi.org/10.1016/j.vetpar.2011.07.037
Galuppi, R. ; Bonoli, C. ; Aureli, S. ; Cassini, R. ; Marcer, F. ; Foley, Janet E ; Tampieri, M. P. / Comparison of diagnostic methods to detect piroplasms in asymptomatic cattle. In: Veterinary Parasitology. 2012 ; Vol. 183, No. 3-4. pp. 364-368.
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