Comparison of chondrogenic potential in equine mesenchymal stromal cells derived from adipose tissue and bone marrow

Martin A. Vidal, Sandra O. Robinson, Mandi J. Lopez, Daniel B. Paulsen, Olga Borkhsenious, Jill R. Johnson, Rustin M. Moore, Jeffrey M. Gimble

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Objective To compare the chondrogenic potential of adult equine mesenchymal stem cells derived from bone marrow (MSCs) or adipose tissue (ASCs). Study Design In vitro experimental study. Animals Adult Thoroughbred horses (n=11). Methods BM (5 horses; mean [±SD] age, 4±1.4 years) or adipose tissue (6 horses; mean age, 3.5±1.1 years) samples were obtained. Cryopreserved MSCs and ASCs were used for pellet cultures in stromal medium (C) or induced into chondrogenesis±transforming growth factor-3 (TGFβ3) and bone morphogenic factor-6 (BMP-6). Pellets harvested after 3, 7, 14, and 21 days were examined for cross-sectional size and tissue composition (hematoxylin and eosin), glycosaminoglycan (GAG) staining (Alcian blue), collagen type II immunohistochemistry, and by transmission electron microscopy. Pellet GAG and total DNA content were measured using dimethylmethylene blue and Hoechst DNA assays. Results Collagen type II synthesis was predominantly observed in MSC pellets from Day 7 onward. Unlike ASC cultures, MSC pellets had hyaline-like matrix by Day 14. GAG deposition occurred earlier in MSC cultures compared with ASC cultures and growth factors enhanced both MSC GAG concentrations (P<.0001) and MSC pellet size (P<.004) after 2 weeks in culture. Conclusion Equine MSCs have superior chondrogenic potential compared with ASCs and the equine ASC growth factor response suggests possible differences compared with other species. Clinical Relevance Elucidation of equine ASC and MSC receptor profiles will enhance the use of these cells in regenerative cartilage repair.

Original languageEnglish (US)
Pages (from-to)713-724
Number of pages12
JournalVeterinary Surgery
Volume37
Issue number8
DOIs
StatePublished - Dec 2008

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stromal cells
Mesenchymal Stromal Cells
bone marrow
adipose tissue
Horses
Adipose Tissue
Bone Marrow
pellets
horses
glycosaminoglycans
Glycosaminoglycans
growth factors
Intercellular Signaling Peptides and Proteins
Collagen Type II
collagen
Alcian Blue
Hyalin
DNA
Hematoxylin
Eosine Yellowish-(YS)

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Vidal, M. A., Robinson, S. O., Lopez, M. J., Paulsen, D. B., Borkhsenious, O., Johnson, J. R., ... Gimble, J. M. (2008). Comparison of chondrogenic potential in equine mesenchymal stromal cells derived from adipose tissue and bone marrow. Veterinary Surgery, 37(8), 713-724. https://doi.org/10.1111/j.1532-950X.2008.00462.x

Comparison of chondrogenic potential in equine mesenchymal stromal cells derived from adipose tissue and bone marrow. / Vidal, Martin A.; Robinson, Sandra O.; Lopez, Mandi J.; Paulsen, Daniel B.; Borkhsenious, Olga; Johnson, Jill R.; Moore, Rustin M.; Gimble, Jeffrey M.

In: Veterinary Surgery, Vol. 37, No. 8, 12.2008, p. 713-724.

Research output: Contribution to journalArticle

Vidal, MA, Robinson, SO, Lopez, MJ, Paulsen, DB, Borkhsenious, O, Johnson, JR, Moore, RM & Gimble, JM 2008, 'Comparison of chondrogenic potential in equine mesenchymal stromal cells derived from adipose tissue and bone marrow', Veterinary Surgery, vol. 37, no. 8, pp. 713-724. https://doi.org/10.1111/j.1532-950X.2008.00462.x
Vidal, Martin A. ; Robinson, Sandra O. ; Lopez, Mandi J. ; Paulsen, Daniel B. ; Borkhsenious, Olga ; Johnson, Jill R. ; Moore, Rustin M. ; Gimble, Jeffrey M. / Comparison of chondrogenic potential in equine mesenchymal stromal cells derived from adipose tissue and bone marrow. In: Veterinary Surgery. 2008 ; Vol. 37, No. 8. pp. 713-724.
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AU - Borkhsenious, Olga

AU - Johnson, Jill R.

AU - Moore, Rustin M.

AU - Gimble, Jeffrey M.

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N2 - Objective To compare the chondrogenic potential of adult equine mesenchymal stem cells derived from bone marrow (MSCs) or adipose tissue (ASCs). Study Design In vitro experimental study. Animals Adult Thoroughbred horses (n=11). Methods BM (5 horses; mean [±SD] age, 4±1.4 years) or adipose tissue (6 horses; mean age, 3.5±1.1 years) samples were obtained. Cryopreserved MSCs and ASCs were used for pellet cultures in stromal medium (C) or induced into chondrogenesis±transforming growth factor-3 (TGFβ3) and bone morphogenic factor-6 (BMP-6). Pellets harvested after 3, 7, 14, and 21 days were examined for cross-sectional size and tissue composition (hematoxylin and eosin), glycosaminoglycan (GAG) staining (Alcian blue), collagen type II immunohistochemistry, and by transmission electron microscopy. Pellet GAG and total DNA content were measured using dimethylmethylene blue and Hoechst DNA assays. Results Collagen type II synthesis was predominantly observed in MSC pellets from Day 7 onward. Unlike ASC cultures, MSC pellets had hyaline-like matrix by Day 14. GAG deposition occurred earlier in MSC cultures compared with ASC cultures and growth factors enhanced both MSC GAG concentrations (P<.0001) and MSC pellet size (P<.004) after 2 weeks in culture. Conclusion Equine MSCs have superior chondrogenic potential compared with ASCs and the equine ASC growth factor response suggests possible differences compared with other species. Clinical Relevance Elucidation of equine ASC and MSC receptor profiles will enhance the use of these cells in regenerative cartilage repair.

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