Comparison of Chlamydia psittaci from cats with upper respiratory tract disease by polymerase chain reaction analysis of the ompA gene

Jane E Sykes, V. P. Studdert, G. Anderson, G. F. Browning

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Conjunctival swabs were taken from 168 cats with clinical signs of acute or chronic upper respiratory tract disease and tested for Chlamydia psittaci by the polymerase chain reaction (PCR) to amplify the ompA gene coding region. Twenty-two (13 per cent) were positive for C psittaci. The PCR products from positive samples were subjected to restriction endonuclease analysis with the restriction enzymes Alu I and Mse I. The fragments of DNA were detected on silver-stained polyacryl-amide gels and the results were compared with the results obtained from chlamydial isolates from cats in Japan, France, the USA and the UK. All the strains had identical restriction patterns. When PCR is used as an epidemiological tool, feline chlamydial strains worldwide appear very similar.

Original languageEnglish (US)
Pages (from-to)310-313
Number of pages4
JournalVeterinary Record
Volume140
Issue number12
StatePublished - 1997
Externally publishedYes

Fingerprint

Chlamydophila psittaci
Respiratory Tract Diseases
respiratory tract diseases
Cats
polymerase chain reaction
cats
Polymerase Chain Reaction
Genes
Restriction Mapping
restriction mapping
genes
Felidae
DNA Restriction Enzymes
amides
Silver
Amides
silver
France
Japan
Gels

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Comparison of Chlamydia psittaci from cats with upper respiratory tract disease by polymerase chain reaction analysis of the ompA gene. / Sykes, Jane E; Studdert, V. P.; Anderson, G.; Browning, G. F.

In: Veterinary Record, Vol. 140, No. 12, 1997, p. 310-313.

Research output: Contribution to journalArticle

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