Comparison of a Time-Resolved Fluorescence Immunoassay and an Enzyme-Linked Immunosorbent Assay for the Analysis of Atrazine in Water

Gerry J. Reimer, Shirley J. Gee, Bruce D. Hammock

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Immunoassays for atrazine based on a time-resolved fluorescent label and an enzyme label were optimized and utilized to measure atrazine in water. The time-resolved fluorescent immunoassay (TRFIA) was based on a polyclonal antibody and a europium label, whereas the enzyme-linked immunosorbent assay (ELISA) utilized a monoclonal antibody and horseradish peroxidase as the label. Detection limits and IC50 values calculated from standard curves were 0.05 ± 0.03 and 0.17 ± 0.08 ng/mL (n = 8) for the TRFIA, respectively, and 0.05 ± 0.04 and 0.3 ± 0.2 ng/mL (n = 17) for the ELISA, respectively. Four different environmental water samples were fortified at various levels of atrazine. When these samples were analyzed, the % RSD for replicate fluorescence or absorbance readings was small (5 and 6%, respectively). The average accuracies for the TRFIA and ELISA were 1.4 ± 0.42 (n = 13) and 1.0 ± 0.38 (n = 13), respectively, reflecting the slight bias of the TRFIA. TRFIA offers an advantage over ELISA in that the fluorescent label is less susceptible to interferences that inhibit enzyme activity and reagents may be more stable than enzyme reagents.

Original languageEnglish (US)
Pages (from-to)3353-3358
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume46
Issue number8
StatePublished - Aug 1998

Keywords

  • Atrazine
  • Enzyme-linked immunosorbent assay
  • Herbicide
  • Immunoassay
  • Time-resolved fluorescence
  • Triazine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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