Comparison of a Salmonella Enteritidis-specific polymerase chain reaction assay to delayed secondary enrichment culture for the detection of Salmonella Enteritidis in environmental drag swab samples

Bruce R. Charlton, Richard L. Walker, Hailu Kinde, Cathryn R. Bauer, Sally E. Channing-Santiago, Thomas B Farver

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The California Egg Quality Assurance Program uses the delayed secondary enrichment culture method for detecting Salmonella Enteritidis in environmental drag swabs obtained from commercial layer complexes. The turnaround time for this method is variable and is dependent on the prevalence of Salmonella, level of the Salmonella identification, and capabilities of the performing laboratory. On a sample basis, a range of 4 to 8 days is required to identify a Salmonella sp. to the serogroup level. Additional time is required to serotype group D Salmonella isolates. A Salmonella Enteritidis-specific polymerase chain reaction (PCR) assay was developed for use on drag swabs and chick box papers, and had a turnaround time of 3-4 days. The delayed secondary enrichment culture method and the Salmonella Enteritidis-specific PCR assay were compared on 942 drag swab and 85 chick box paper samples submitted from 217 and 22 premises, respectively, as part of the California Egg Quality Assurance Program. The PCR assay identified 43 positive Salmonella Enteritidis samples from 22 premises, whereas the culture method identified 24 group D Salmonella-positive samples from 16 premises. There was a significant difference (P = 0.001) in the proportion of positive samples as determined by the two assays. Complete serotyping of the group D Salmonella-positive cultures confirmed Salmonella Enteritidis in all but one sample that was identified as Salmonella Jamaica and was negative by the PCR assay.

Original languageEnglish (US)
Pages (from-to)418-422
Number of pages5
JournalAvian Diseases
Volume49
Issue number3
StatePublished - Sep 2005

Fingerprint

Salmonella enteritidis
enrichment culture
Salmonella Enteritidis
Salmonella
polymerase chain reaction
Polymerase Chain Reaction
assays
sampling
egg quality
quality control
Ovum
serotypes
chicks
Jamaica
Serotyping
methodology

Keywords

  • Drag swab
  • Environment
  • Quality assurance
  • Salmonella Enteritidis

ASJC Scopus subject areas

  • Immunology
  • Cancer Research
  • veterinary(all)

Cite this

Comparison of a Salmonella Enteritidis-specific polymerase chain reaction assay to delayed secondary enrichment culture for the detection of Salmonella Enteritidis in environmental drag swab samples. / Charlton, Bruce R.; Walker, Richard L.; Kinde, Hailu; Bauer, Cathryn R.; Channing-Santiago, Sally E.; Farver, Thomas B.

In: Avian Diseases, Vol. 49, No. 3, 09.2005, p. 418-422.

Research output: Contribution to journalArticle

@article{3020a11fba4d498aa88a4e6dd20a2f3a,
title = "Comparison of a Salmonella Enteritidis-specific polymerase chain reaction assay to delayed secondary enrichment culture for the detection of Salmonella Enteritidis in environmental drag swab samples",
abstract = "The California Egg Quality Assurance Program uses the delayed secondary enrichment culture method for detecting Salmonella Enteritidis in environmental drag swabs obtained from commercial layer complexes. The turnaround time for this method is variable and is dependent on the prevalence of Salmonella, level of the Salmonella identification, and capabilities of the performing laboratory. On a sample basis, a range of 4 to 8 days is required to identify a Salmonella sp. to the serogroup level. Additional time is required to serotype group D Salmonella isolates. A Salmonella Enteritidis-specific polymerase chain reaction (PCR) assay was developed for use on drag swabs and chick box papers, and had a turnaround time of 3-4 days. The delayed secondary enrichment culture method and the Salmonella Enteritidis-specific PCR assay were compared on 942 drag swab and 85 chick box paper samples submitted from 217 and 22 premises, respectively, as part of the California Egg Quality Assurance Program. The PCR assay identified 43 positive Salmonella Enteritidis samples from 22 premises, whereas the culture method identified 24 group D Salmonella-positive samples from 16 premises. There was a significant difference (P = 0.001) in the proportion of positive samples as determined by the two assays. Complete serotyping of the group D Salmonella-positive cultures confirmed Salmonella Enteritidis in all but one sample that was identified as Salmonella Jamaica and was negative by the PCR assay.",
keywords = "Drag swab, Environment, Quality assurance, Salmonella Enteritidis",
author = "Charlton, {Bruce R.} and Walker, {Richard L.} and Hailu Kinde and Bauer, {Cathryn R.} and Channing-Santiago, {Sally E.} and Farver, {Thomas B}",
year = "2005",
month = "9",
language = "English (US)",
volume = "49",
pages = "418--422",
journal = "Avian Diseases",
issn = "0005-2086",
publisher = "American Association of Avian Pathologists",
number = "3",

}

TY - JOUR

T1 - Comparison of a Salmonella Enteritidis-specific polymerase chain reaction assay to delayed secondary enrichment culture for the detection of Salmonella Enteritidis in environmental drag swab samples

AU - Charlton, Bruce R.

AU - Walker, Richard L.

AU - Kinde, Hailu

AU - Bauer, Cathryn R.

AU - Channing-Santiago, Sally E.

AU - Farver, Thomas B

PY - 2005/9

Y1 - 2005/9

N2 - The California Egg Quality Assurance Program uses the delayed secondary enrichment culture method for detecting Salmonella Enteritidis in environmental drag swabs obtained from commercial layer complexes. The turnaround time for this method is variable and is dependent on the prevalence of Salmonella, level of the Salmonella identification, and capabilities of the performing laboratory. On a sample basis, a range of 4 to 8 days is required to identify a Salmonella sp. to the serogroup level. Additional time is required to serotype group D Salmonella isolates. A Salmonella Enteritidis-specific polymerase chain reaction (PCR) assay was developed for use on drag swabs and chick box papers, and had a turnaround time of 3-4 days. The delayed secondary enrichment culture method and the Salmonella Enteritidis-specific PCR assay were compared on 942 drag swab and 85 chick box paper samples submitted from 217 and 22 premises, respectively, as part of the California Egg Quality Assurance Program. The PCR assay identified 43 positive Salmonella Enteritidis samples from 22 premises, whereas the culture method identified 24 group D Salmonella-positive samples from 16 premises. There was a significant difference (P = 0.001) in the proportion of positive samples as determined by the two assays. Complete serotyping of the group D Salmonella-positive cultures confirmed Salmonella Enteritidis in all but one sample that was identified as Salmonella Jamaica and was negative by the PCR assay.

AB - The California Egg Quality Assurance Program uses the delayed secondary enrichment culture method for detecting Salmonella Enteritidis in environmental drag swabs obtained from commercial layer complexes. The turnaround time for this method is variable and is dependent on the prevalence of Salmonella, level of the Salmonella identification, and capabilities of the performing laboratory. On a sample basis, a range of 4 to 8 days is required to identify a Salmonella sp. to the serogroup level. Additional time is required to serotype group D Salmonella isolates. A Salmonella Enteritidis-specific polymerase chain reaction (PCR) assay was developed for use on drag swabs and chick box papers, and had a turnaround time of 3-4 days. The delayed secondary enrichment culture method and the Salmonella Enteritidis-specific PCR assay were compared on 942 drag swab and 85 chick box paper samples submitted from 217 and 22 premises, respectively, as part of the California Egg Quality Assurance Program. The PCR assay identified 43 positive Salmonella Enteritidis samples from 22 premises, whereas the culture method identified 24 group D Salmonella-positive samples from 16 premises. There was a significant difference (P = 0.001) in the proportion of positive samples as determined by the two assays. Complete serotyping of the group D Salmonella-positive cultures confirmed Salmonella Enteritidis in all but one sample that was identified as Salmonella Jamaica and was negative by the PCR assay.

KW - Drag swab

KW - Environment

KW - Quality assurance

KW - Salmonella Enteritidis

UR - http://www.scopus.com/inward/record.url?scp=24944557991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24944557991&partnerID=8YFLogxK

M3 - Article

C2 - 16252498

AN - SCOPUS:24944557991

VL - 49

SP - 418

EP - 422

JO - Avian Diseases

JF - Avian Diseases

SN - 0005-2086

IS - 3

ER -