Comparison of 2 homogeneous high-density lipoprotein cholesterol assays

Mai P. Hoang, Shaina V. Hirany, Joseph Parupia, Sridevi Devaraj, Ishwarlal Jialal

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background. High-density lipoprotein cholesterol (HDL-C) is an independent inverse risk factor for coronary artery disease. Current methodologies for measurement of HDL-C in most clinical laboratories involve chemical precipitation-based methods. However, these methods are time- consuming, affected by high triglycerides, are not suitable for complete automation, and require a large sample size. New direct homogeneous methods are now available that do not have these constraints. Design.-We evaluated the performance of 2 direct homogeneous methods, Liquid N-geneous HDL-C assay (LNHDL) and Boehringer Mannheim HDL Cholesterol assay (BM-HDL), and compared these methods against a modified Centers for Disease Control and Prevention reference method (MR-HDL) in 126 patients with normotriglyceridemia (triglycerides < 4.5 mmol/L, range 0.6-4.3 mmol/L) and 50 patients with hypertriglyceridemia (triglycerides ≥ 4.5 mmol/L, range 4.5-18.8 mmol/L). Results.-Excellent precision profiles were exhibited by both homogeneous methods. Both LN-HDL and BM-HDL correlated well with MR-HDL in normotriglyceridemia (r = 0.98, slope = 0.93 and r = 0.97, slope = 1.0, respectively). However, compared with the modified reference method, the LN- HDL correlated better than the BM-HDL in hypertriglyceridemic samples (r = 0.97, slope = 1.0 and r = 0.91, slope = 0.9, respectively). The 1998 National Cholesterol Education Program guidelines for accuracy (bias < ±5%) were met by LN-HDL in both normotriglyceridemic and hypertriglyceridemic samples (bias = 1.3% and 3.3%, respectively); however, BM-HDL failed to meet the National Cholesterol Education Program accuracy criteria in both triglyceride subgroups (bias = 8.2% and 11.3%, respectively). In addition, the total error for LNHDL in both normotriglyceridemia (6.6%) and hypertriglyceridemia (8.6%) was well within the National Cholesterol Education Program guidelines for total error (≤13%); BM-HDL exhibited a higher total error than LN-HDL in normotriglyceridemia (11.9%) and failed to meet the National Cholesterol Education Program guidelines in hypertriglyceridemia (15.0%). Conclusion.- Although both homogeneous methods are precise, the LN-HDL assay is superior in accuracy to the BM-HDL assay when compared with the modified reference method.

Original languageEnglish (US)
Pages (from-to)1005-1009
Number of pages5
JournalArchives of Pathology and Laboratory Medicine
Volume122
Issue number11
StatePublished - Nov 1998
Externally publishedYes

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HDL Cholesterol
Hypertriglyceridemia
Triglycerides
Cholesterol
Education
Guidelines
Chemical Precipitation
Laboratory Chemicals
Automation
Centers for Disease Control and Prevention (U.S.)
Sample Size
Coronary Artery Disease

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Hoang, M. P., Hirany, S. V., Parupia, J., Devaraj, S., & Jialal, I. (1998). Comparison of 2 homogeneous high-density lipoprotein cholesterol assays. Archives of Pathology and Laboratory Medicine, 122(11), 1005-1009.

Comparison of 2 homogeneous high-density lipoprotein cholesterol assays. / Hoang, Mai P.; Hirany, Shaina V.; Parupia, Joseph; Devaraj, Sridevi; Jialal, Ishwarlal.

In: Archives of Pathology and Laboratory Medicine, Vol. 122, No. 11, 11.1998, p. 1005-1009.

Research output: Contribution to journalArticle

Hoang, MP, Hirany, SV, Parupia, J, Devaraj, S & Jialal, I 1998, 'Comparison of 2 homogeneous high-density lipoprotein cholesterol assays', Archives of Pathology and Laboratory Medicine, vol. 122, no. 11, pp. 1005-1009.
Hoang MP, Hirany SV, Parupia J, Devaraj S, Jialal I. Comparison of 2 homogeneous high-density lipoprotein cholesterol assays. Archives of Pathology and Laboratory Medicine. 1998 Nov;122(11):1005-1009.
Hoang, Mai P. ; Hirany, Shaina V. ; Parupia, Joseph ; Devaraj, Sridevi ; Jialal, Ishwarlal. / Comparison of 2 homogeneous high-density lipoprotein cholesterol assays. In: Archives of Pathology and Laboratory Medicine. 1998 ; Vol. 122, No. 11. pp. 1005-1009.
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title = "Comparison of 2 homogeneous high-density lipoprotein cholesterol assays",
abstract = "Background. High-density lipoprotein cholesterol (HDL-C) is an independent inverse risk factor for coronary artery disease. Current methodologies for measurement of HDL-C in most clinical laboratories involve chemical precipitation-based methods. However, these methods are time- consuming, affected by high triglycerides, are not suitable for complete automation, and require a large sample size. New direct homogeneous methods are now available that do not have these constraints. Design.-We evaluated the performance of 2 direct homogeneous methods, Liquid N-geneous HDL-C assay (LNHDL) and Boehringer Mannheim HDL Cholesterol assay (BM-HDL), and compared these methods against a modified Centers for Disease Control and Prevention reference method (MR-HDL) in 126 patients with normotriglyceridemia (triglycerides < 4.5 mmol/L, range 0.6-4.3 mmol/L) and 50 patients with hypertriglyceridemia (triglycerides ≥ 4.5 mmol/L, range 4.5-18.8 mmol/L). Results.-Excellent precision profiles were exhibited by both homogeneous methods. Both LN-HDL and BM-HDL correlated well with MR-HDL in normotriglyceridemia (r = 0.98, slope = 0.93 and r = 0.97, slope = 1.0, respectively). However, compared with the modified reference method, the LN- HDL correlated better than the BM-HDL in hypertriglyceridemic samples (r = 0.97, slope = 1.0 and r = 0.91, slope = 0.9, respectively). The 1998 National Cholesterol Education Program guidelines for accuracy (bias < ±5{\%}) were met by LN-HDL in both normotriglyceridemic and hypertriglyceridemic samples (bias = 1.3{\%} and 3.3{\%}, respectively); however, BM-HDL failed to meet the National Cholesterol Education Program accuracy criteria in both triglyceride subgroups (bias = 8.2{\%} and 11.3{\%}, respectively). In addition, the total error for LNHDL in both normotriglyceridemia (6.6{\%}) and hypertriglyceridemia (8.6{\%}) was well within the National Cholesterol Education Program guidelines for total error (≤13{\%}); BM-HDL exhibited a higher total error than LN-HDL in normotriglyceridemia (11.9{\%}) and failed to meet the National Cholesterol Education Program guidelines in hypertriglyceridemia (15.0{\%}). Conclusion.- Although both homogeneous methods are precise, the LN-HDL assay is superior in accuracy to the BM-HDL assay when compared with the modified reference method.",
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N2 - Background. High-density lipoprotein cholesterol (HDL-C) is an independent inverse risk factor for coronary artery disease. Current methodologies for measurement of HDL-C in most clinical laboratories involve chemical precipitation-based methods. However, these methods are time- consuming, affected by high triglycerides, are not suitable for complete automation, and require a large sample size. New direct homogeneous methods are now available that do not have these constraints. Design.-We evaluated the performance of 2 direct homogeneous methods, Liquid N-geneous HDL-C assay (LNHDL) and Boehringer Mannheim HDL Cholesterol assay (BM-HDL), and compared these methods against a modified Centers for Disease Control and Prevention reference method (MR-HDL) in 126 patients with normotriglyceridemia (triglycerides < 4.5 mmol/L, range 0.6-4.3 mmol/L) and 50 patients with hypertriglyceridemia (triglycerides ≥ 4.5 mmol/L, range 4.5-18.8 mmol/L). Results.-Excellent precision profiles were exhibited by both homogeneous methods. Both LN-HDL and BM-HDL correlated well with MR-HDL in normotriglyceridemia (r = 0.98, slope = 0.93 and r = 0.97, slope = 1.0, respectively). However, compared with the modified reference method, the LN- HDL correlated better than the BM-HDL in hypertriglyceridemic samples (r = 0.97, slope = 1.0 and r = 0.91, slope = 0.9, respectively). The 1998 National Cholesterol Education Program guidelines for accuracy (bias < ±5%) were met by LN-HDL in both normotriglyceridemic and hypertriglyceridemic samples (bias = 1.3% and 3.3%, respectively); however, BM-HDL failed to meet the National Cholesterol Education Program accuracy criteria in both triglyceride subgroups (bias = 8.2% and 11.3%, respectively). In addition, the total error for LNHDL in both normotriglyceridemia (6.6%) and hypertriglyceridemia (8.6%) was well within the National Cholesterol Education Program guidelines for total error (≤13%); BM-HDL exhibited a higher total error than LN-HDL in normotriglyceridemia (11.9%) and failed to meet the National Cholesterol Education Program guidelines in hypertriglyceridemia (15.0%). Conclusion.- Although both homogeneous methods are precise, the LN-HDL assay is superior in accuracy to the BM-HDL assay when compared with the modified reference method.

AB - Background. High-density lipoprotein cholesterol (HDL-C) is an independent inverse risk factor for coronary artery disease. Current methodologies for measurement of HDL-C in most clinical laboratories involve chemical precipitation-based methods. However, these methods are time- consuming, affected by high triglycerides, are not suitable for complete automation, and require a large sample size. New direct homogeneous methods are now available that do not have these constraints. Design.-We evaluated the performance of 2 direct homogeneous methods, Liquid N-geneous HDL-C assay (LNHDL) and Boehringer Mannheim HDL Cholesterol assay (BM-HDL), and compared these methods against a modified Centers for Disease Control and Prevention reference method (MR-HDL) in 126 patients with normotriglyceridemia (triglycerides < 4.5 mmol/L, range 0.6-4.3 mmol/L) and 50 patients with hypertriglyceridemia (triglycerides ≥ 4.5 mmol/L, range 4.5-18.8 mmol/L). Results.-Excellent precision profiles were exhibited by both homogeneous methods. Both LN-HDL and BM-HDL correlated well with MR-HDL in normotriglyceridemia (r = 0.98, slope = 0.93 and r = 0.97, slope = 1.0, respectively). However, compared with the modified reference method, the LN- HDL correlated better than the BM-HDL in hypertriglyceridemic samples (r = 0.97, slope = 1.0 and r = 0.91, slope = 0.9, respectively). The 1998 National Cholesterol Education Program guidelines for accuracy (bias < ±5%) were met by LN-HDL in both normotriglyceridemic and hypertriglyceridemic samples (bias = 1.3% and 3.3%, respectively); however, BM-HDL failed to meet the National Cholesterol Education Program accuracy criteria in both triglyceride subgroups (bias = 8.2% and 11.3%, respectively). In addition, the total error for LNHDL in both normotriglyceridemia (6.6%) and hypertriglyceridemia (8.6%) was well within the National Cholesterol Education Program guidelines for total error (≤13%); BM-HDL exhibited a higher total error than LN-HDL in normotriglyceridemia (11.9%) and failed to meet the National Cholesterol Education Program guidelines in hypertriglyceridemia (15.0%). Conclusion.- Although both homogeneous methods are precise, the LN-HDL assay is superior in accuracy to the BM-HDL assay when compared with the modified reference method.

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