Comparison of 18S and ITS-1 rDNA sequences of selected geographic isolates of Myxobolus cerebralis

Karl B. Andree, Mansour El-Matbouli, Rudolph W. Hoffman, Ronald Hedrick

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Myxobolus cerebralis, the myxosporean parasite-causing salmonid whirling disease, was first reported among rainbow trout (Oncorhynchus mykiss) in Germany in 1903. The parasite was reported for the first time in North America in 1958 among hatchery-reared trout in the eastern USA, presumably arriving with frozen trout shipments from Europe. A comparison of 18S and ITS-1 ribosomal DNA sequences was conducted to identify potential strain differences between selected geographic isolates of this parasite from Europe and North America. Only fourteen of 1700 base pairs were different in the 18S rRNA gene from isolates obtained from California and West Virginia in the USA, and the Federal German Republic. No evidence for strain differences was obtained from ITS-1 sequences that were found to be identical among all parasite isolates. This finding is consistent with the hypothesis that the parasite was recently introduced to the USA from Europe.

Original languageEnglish (US)
Pages (from-to)771-775
Number of pages5
JournalInternational Journal for Parasitology
Volume29
Issue number5
DOIs
StatePublished - May 1 1999

Fingerprint

Myxobolus
Ribosomal DNA
Parasites
Trout
Oncorhynchus mykiss
North America
rRNA Genes
Base Pairing
Germany

Keywords

  • 18S
  • ITS-1
  • Myxobolus cerebralis
  • Ribosomal DNA
  • Salmonids
  • Whirling disease

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Comparison of 18S and ITS-1 rDNA sequences of selected geographic isolates of Myxobolus cerebralis. / Andree, Karl B.; El-Matbouli, Mansour; Hoffman, Rudolph W.; Hedrick, Ronald.

In: International Journal for Parasitology, Vol. 29, No. 5, 01.05.1999, p. 771-775.

Research output: Contribution to journalArticle

Andree, Karl B. ; El-Matbouli, Mansour ; Hoffman, Rudolph W. ; Hedrick, Ronald. / Comparison of 18S and ITS-1 rDNA sequences of selected geographic isolates of Myxobolus cerebralis. In: International Journal for Parasitology. 1999 ; Vol. 29, No. 5. pp. 771-775.
@article{3e82ecd8edec498ba0dee0a7a4484042,
title = "Comparison of 18S and ITS-1 rDNA sequences of selected geographic isolates of Myxobolus cerebralis",
abstract = "Myxobolus cerebralis, the myxosporean parasite-causing salmonid whirling disease, was first reported among rainbow trout (Oncorhynchus mykiss) in Germany in 1903. The parasite was reported for the first time in North America in 1958 among hatchery-reared trout in the eastern USA, presumably arriving with frozen trout shipments from Europe. A comparison of 18S and ITS-1 ribosomal DNA sequences was conducted to identify potential strain differences between selected geographic isolates of this parasite from Europe and North America. Only fourteen of 1700 base pairs were different in the 18S rRNA gene from isolates obtained from California and West Virginia in the USA, and the Federal German Republic. No evidence for strain differences was obtained from ITS-1 sequences that were found to be identical among all parasite isolates. This finding is consistent with the hypothesis that the parasite was recently introduced to the USA from Europe.",
keywords = "18S, ITS-1, Myxobolus cerebralis, Ribosomal DNA, Salmonids, Whirling disease",
author = "Andree, {Karl B.} and Mansour El-Matbouli and Hoffman, {Rudolph W.} and Ronald Hedrick",
year = "1999",
month = "5",
day = "1",
doi = "10.1016/S0020-7519(99)00035-1",
language = "English (US)",
volume = "29",
pages = "771--775",
journal = "International Journal for Parasitology",
issn = "0020-7519",
publisher = "Elsevier Limited",
number = "5",

}

TY - JOUR

T1 - Comparison of 18S and ITS-1 rDNA sequences of selected geographic isolates of Myxobolus cerebralis

AU - Andree, Karl B.

AU - El-Matbouli, Mansour

AU - Hoffman, Rudolph W.

AU - Hedrick, Ronald

PY - 1999/5/1

Y1 - 1999/5/1

N2 - Myxobolus cerebralis, the myxosporean parasite-causing salmonid whirling disease, was first reported among rainbow trout (Oncorhynchus mykiss) in Germany in 1903. The parasite was reported for the first time in North America in 1958 among hatchery-reared trout in the eastern USA, presumably arriving with frozen trout shipments from Europe. A comparison of 18S and ITS-1 ribosomal DNA sequences was conducted to identify potential strain differences between selected geographic isolates of this parasite from Europe and North America. Only fourteen of 1700 base pairs were different in the 18S rRNA gene from isolates obtained from California and West Virginia in the USA, and the Federal German Republic. No evidence for strain differences was obtained from ITS-1 sequences that were found to be identical among all parasite isolates. This finding is consistent with the hypothesis that the parasite was recently introduced to the USA from Europe.

AB - Myxobolus cerebralis, the myxosporean parasite-causing salmonid whirling disease, was first reported among rainbow trout (Oncorhynchus mykiss) in Germany in 1903. The parasite was reported for the first time in North America in 1958 among hatchery-reared trout in the eastern USA, presumably arriving with frozen trout shipments from Europe. A comparison of 18S and ITS-1 ribosomal DNA sequences was conducted to identify potential strain differences between selected geographic isolates of this parasite from Europe and North America. Only fourteen of 1700 base pairs were different in the 18S rRNA gene from isolates obtained from California and West Virginia in the USA, and the Federal German Republic. No evidence for strain differences was obtained from ITS-1 sequences that were found to be identical among all parasite isolates. This finding is consistent with the hypothesis that the parasite was recently introduced to the USA from Europe.

KW - 18S

KW - ITS-1

KW - Myxobolus cerebralis

KW - Ribosomal DNA

KW - Salmonids

KW - Whirling disease

UR - http://www.scopus.com/inward/record.url?scp=0032986735&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032986735&partnerID=8YFLogxK

U2 - 10.1016/S0020-7519(99)00035-1

DO - 10.1016/S0020-7519(99)00035-1

M3 - Article

VL - 29

SP - 771

EP - 775

JO - International Journal for Parasitology

JF - International Journal for Parasitology

SN - 0020-7519

IS - 5

ER -