Comparing Raman and fluorescence lifetime spectroscopy from human atherosclerotic lesions using a bimodal probe

Sebastian Dochow, Hussain Fatakdawala, Jennifer E. Phipps, Dinglong Ma, Thomas Bocklitz, Michael Schmitt, John W Bishop, Kenneth B. Margulies, Laura Marcu, Jürgen Popp

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Fluorescence lifetime imaging (FLIm) and Raman spectroscopy are two promising methods to support morphological intravascular imaging techniques with chemical contrast. Both approaches are complementary and may also be used in combination with OCT/IVUS to add chemical specificity to these morphologic intravascular imaging modalities. In this contribution, both modalities were simultaneously acquired from two human coronary specimens using a bimodal probe. A previously trained SVM model was used to interpret the fluorescence lifetime data; integrated band intensities displayed in RGB false color images were used to interpret the Raman data. Both modalities demonstrate unique strengths and weaknesses and these will be discussed in comparison to histologic analyses from the two coronary arteries imaged. (Figure presented.).

Original languageEnglish (US)
Pages (from-to)958-966
Number of pages9
JournalJournal of Biophotonics
Volume9
Issue number9
DOIs
StatePublished - Sep 1 2016

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Keywords

  • atherosclerosis
  • bimodal
  • fiber probes
  • fluorescence lifetime
  • imaging
  • Raman spectroscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Dochow, S., Fatakdawala, H., Phipps, J. E., Ma, D., Bocklitz, T., Schmitt, M., Bishop, J. W., Margulies, K. B., Marcu, L., & Popp, J. (2016). Comparing Raman and fluorescence lifetime spectroscopy from human atherosclerotic lesions using a bimodal probe. Journal of Biophotonics, 9(9), 958-966. https://doi.org/10.1002/jbio.201500341