Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria

Daniel Garrido, Santiago Ruiz-Moyano, Danielle G. Lemay, David A. Sela, J. Bruce German, David A. Mills

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Breast milk enhances the predominance of Bifidobacterium species in the infant gut, probably due to its large concentration of human milk oligosaccharides (HMO). Here we screened infant-gut isolates of Bifidobacterium longum subsp. infantis and Bifidobacterium bifidum using individual HMO, and compared the global transcriptomes of representative isolates on major HMO by RNA-seq. While B. infantis displayed homogeneous HMO-utilization patterns, B. bifidum were more diverse and some strains did not use fucosyllactose (FL) or sialyllactose (SL). Transcriptomes of B. bifidum SC555 and B. infantis ATCC 15697 showed that utilization of pooled HMO is similar to neutral HMO, while transcriptomes for growth on FL were more similar to lactose than HMO in B. bifidum. Genes linked to HMO-utilization were upregulated by neutral HMO and SL, but not by FL in both species. In contrast, FL induced the expression of alternative gene clusters in B. infantis. Results also suggest that B. bifidum SC555 does not utilize fucose or sialic acid from HMO. Surprisingly, expression of orthologous genes differed between both bifidobacteria even when grown on identical substrates. This study highlights two major strategies found in Bifidobacterium species to process HMO, and presents detailed information on the close relationship between HMO and infant-gut bifidobacteria.

Original languageEnglish (US)
Article number13517
JournalScientific Reports
Volume5
DOIs
StatePublished - Sep 4 2015
Externally publishedYes

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Bifidobacterium
breast milk
transcriptomics
oligosaccharides
digestive system
milk
Bifidobacterium bifidum
Bifidobacterium longum subsp. infantis
transcriptome
fucose
sialic acids
multigene family
lactose
RNA

ASJC Scopus subject areas

  • General

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Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria. / Garrido, Daniel; Ruiz-Moyano, Santiago; Lemay, Danielle G.; Sela, David A.; German, J. Bruce; Mills, David A.

In: Scientific Reports, Vol. 5, 13517, 04.09.2015.

Research output: Contribution to journalArticle

Garrido, Daniel ; Ruiz-Moyano, Santiago ; Lemay, Danielle G. ; Sela, David A. ; German, J. Bruce ; Mills, David A. / Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria. In: Scientific Reports. 2015 ; Vol. 5.
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