Comparative study on the derivatization of l-α-acetylmethadol metabolites for electron capture gas-liquid chromatography

Derick H Lau, Gary L. Henderson

Research output: Contribution to journalArticle

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Six reagents-trichloroacetyl chloride, trichloroacetic anhydride, pentafluorobenzoyl chloride, heptafluorobutyryl chloride, heptafluorobutyric anhydride, and trifluoroacetic anhydride-were evaluated as potential derivatizing reagents for quantitating the metabolites of l-α-acetylmethadol (LAAM)-noracetylmethadol, dinoracetylmethadol, methadol, and normethadol-by electron capture gas-liquid chromatography. All of the reagents studied reacted quantitatively with all of the metabolites except methadol; however, trichloroacetyl chloride was found to be the most satisfactory general reagent for analyzing these metabolites in biological fluids. A gas-liquid chromatographic method is presented which combines both flame ionization and electron capture detection for quantitating plasma and urine levels of methadone, l-α-acetylmethadol and its metabolites.

Original languageEnglish (US)
Pages (from-to)329-338
Number of pages10
JournalJournal of Chromatography A
Issue numberC
StatePublished - Dec 22 1976


ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

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