TY - JOUR
T1 - Comparative study on the derivatization of l-α-acetylmethadol metabolites for electron capture gas-liquid chromatography
AU - Lau, Derick H
AU - Henderson, Gary L.
PY - 1976/12/22
Y1 - 1976/12/22
N2 - Six reagents-trichloroacetyl chloride, trichloroacetic anhydride, pentafluorobenzoyl chloride, heptafluorobutyryl chloride, heptafluorobutyric anhydride, and trifluoroacetic anhydride-were evaluated as potential derivatizing reagents for quantitating the metabolites of l-α-acetylmethadol (LAAM)-noracetylmethadol, dinoracetylmethadol, methadol, and normethadol-by electron capture gas-liquid chromatography. All of the reagents studied reacted quantitatively with all of the metabolites except methadol; however, trichloroacetyl chloride was found to be the most satisfactory general reagent for analyzing these metabolites in biological fluids. A gas-liquid chromatographic method is presented which combines both flame ionization and electron capture detection for quantitating plasma and urine levels of methadone, l-α-acetylmethadol and its metabolites.
AB - Six reagents-trichloroacetyl chloride, trichloroacetic anhydride, pentafluorobenzoyl chloride, heptafluorobutyryl chloride, heptafluorobutyric anhydride, and trifluoroacetic anhydride-were evaluated as potential derivatizing reagents for quantitating the metabolites of l-α-acetylmethadol (LAAM)-noracetylmethadol, dinoracetylmethadol, methadol, and normethadol-by electron capture gas-liquid chromatography. All of the reagents studied reacted quantitatively with all of the metabolites except methadol; however, trichloroacetyl chloride was found to be the most satisfactory general reagent for analyzing these metabolites in biological fluids. A gas-liquid chromatographic method is presented which combines both flame ionization and electron capture detection for quantitating plasma and urine levels of methadone, l-α-acetylmethadol and its metabolites.
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U2 - 10.1016/S0021-9673(00)87792-4
DO - 10.1016/S0021-9673(00)87792-4
M3 - Article
C2 - 1002792
AN - SCOPUS:0017093734
VL - 129
SP - 329
EP - 338
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - C
ER -