Comparative study on the derivatization of l-α-acetylmethadol metabolites for electron capture gas-liquid chromatography

Derick H Lau, Gary L. Henderson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Six reagents-trichloroacetyl chloride, trichloroacetic anhydride, pentafluorobenzoyl chloride, heptafluorobutyryl chloride, heptafluorobutyric anhydride, and trifluoroacetic anhydride-were evaluated as potential derivatizing reagents for quantitating the metabolites of l-α-acetylmethadol (LAAM)-noracetylmethadol, dinoracetylmethadol, methadol, and normethadol-by electron capture gas-liquid chromatography. All of the reagents studied reacted quantitatively with all of the metabolites except methadol; however, trichloroacetyl chloride was found to be the most satisfactory general reagent for analyzing these metabolites in biological fluids. A gas-liquid chromatographic method is presented which combines both flame ionization and electron capture detection for quantitating plasma and urine levels of methadone, l-α-acetylmethadol and its metabolites.

Original languageEnglish (US)
Pages (from-to)329-338
Number of pages10
JournalJournal of Chromatography A
Volume129
Issue numberC
DOIs
StatePublished - Dec 22 1976

Fingerprint

Methadyl Acetate
Liquid chromatography
Metabolites
Gas chromatography
Gas Chromatography
Electrons
trifluoroacetic anhydride
heptafluorobutyric anhydride
Flame Ionization
Methadone
Ionization
Chlorides
Gases
Urine
Plasmas
Fluids
Liquids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Comparative study on the derivatization of l-α-acetylmethadol metabolites for electron capture gas-liquid chromatography. / Lau, Derick H; Henderson, Gary L.

In: Journal of Chromatography A, Vol. 129, No. C, 22.12.1976, p. 329-338.

Research output: Contribution to journalArticle

@article{337363ae014b4251ab1fc26de09c1184,
title = "Comparative study on the derivatization of l-α-acetylmethadol metabolites for electron capture gas-liquid chromatography",
abstract = "Six reagents-trichloroacetyl chloride, trichloroacetic anhydride, pentafluorobenzoyl chloride, heptafluorobutyryl chloride, heptafluorobutyric anhydride, and trifluoroacetic anhydride-were evaluated as potential derivatizing reagents for quantitating the metabolites of l-α-acetylmethadol (LAAM)-noracetylmethadol, dinoracetylmethadol, methadol, and normethadol-by electron capture gas-liquid chromatography. All of the reagents studied reacted quantitatively with all of the metabolites except methadol; however, trichloroacetyl chloride was found to be the most satisfactory general reagent for analyzing these metabolites in biological fluids. A gas-liquid chromatographic method is presented which combines both flame ionization and electron capture detection for quantitating plasma and urine levels of methadone, l-α-acetylmethadol and its metabolites.",
author = "Lau, {Derick H} and Henderson, {Gary L.}",
year = "1976",
month = "12",
day = "22",
doi = "10.1016/S0021-9673(00)87792-4",
language = "English (US)",
volume = "129",
pages = "329--338",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",
number = "C",

}

TY - JOUR

T1 - Comparative study on the derivatization of l-α-acetylmethadol metabolites for electron capture gas-liquid chromatography

AU - Lau, Derick H

AU - Henderson, Gary L.

PY - 1976/12/22

Y1 - 1976/12/22

N2 - Six reagents-trichloroacetyl chloride, trichloroacetic anhydride, pentafluorobenzoyl chloride, heptafluorobutyryl chloride, heptafluorobutyric anhydride, and trifluoroacetic anhydride-were evaluated as potential derivatizing reagents for quantitating the metabolites of l-α-acetylmethadol (LAAM)-noracetylmethadol, dinoracetylmethadol, methadol, and normethadol-by electron capture gas-liquid chromatography. All of the reagents studied reacted quantitatively with all of the metabolites except methadol; however, trichloroacetyl chloride was found to be the most satisfactory general reagent for analyzing these metabolites in biological fluids. A gas-liquid chromatographic method is presented which combines both flame ionization and electron capture detection for quantitating plasma and urine levels of methadone, l-α-acetylmethadol and its metabolites.

AB - Six reagents-trichloroacetyl chloride, trichloroacetic anhydride, pentafluorobenzoyl chloride, heptafluorobutyryl chloride, heptafluorobutyric anhydride, and trifluoroacetic anhydride-were evaluated as potential derivatizing reagents for quantitating the metabolites of l-α-acetylmethadol (LAAM)-noracetylmethadol, dinoracetylmethadol, methadol, and normethadol-by electron capture gas-liquid chromatography. All of the reagents studied reacted quantitatively with all of the metabolites except methadol; however, trichloroacetyl chloride was found to be the most satisfactory general reagent for analyzing these metabolites in biological fluids. A gas-liquid chromatographic method is presented which combines both flame ionization and electron capture detection for quantitating plasma and urine levels of methadone, l-α-acetylmethadol and its metabolites.

UR - http://www.scopus.com/inward/record.url?scp=0017093734&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017093734&partnerID=8YFLogxK

U2 - 10.1016/S0021-9673(00)87792-4

DO - 10.1016/S0021-9673(00)87792-4

M3 - Article

VL - 129

SP - 329

EP - 338

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

IS - C

ER -