Abstract
Six reagents-trichloroacetyl chloride, trichloroacetic anhydride, pentafluorobenzoyl chloride, heptafluorobutyryl chloride, heptafluorobutyric anhydride, and trifluoroacetic anhydride-were evaluated as potential derivatizing reagents for quantitating the metabolites of l-α-acetylmethadol (LAAM)-noracetylmethadol, dinoracetylmethadol, methadol, and normethadol-by electron capture gas-liquid chromatography. All of the reagents studied reacted quantitatively with all of the metabolites except methadol; however, trichloroacetyl chloride was found to be the most satisfactory general reagent for analyzing these metabolites in biological fluids. A gas-liquid chromatographic method is presented which combines both flame ionization and electron capture detection for quantitating plasma and urine levels of methadone, l-α-acetylmethadol and its metabolites.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 329-338 |
| Number of pages | 10 |
| Journal | Journal of Chromatography A |
| Volume | 129 |
| Issue number | C |
| DOIs | |
| State | Published - Dec 22 1976 |
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ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry
- Molecular Medicine
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Comparative study on the derivatization of l-α-acetylmethadol metabolites for electron capture gas-liquid chromatography. / Lau, Derick H; Henderson, Gary L.
In: Journal of Chromatography A, Vol. 129, No. C, 22.12.1976, p. 329-338.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative study on the derivatization of l-α-acetylmethadol metabolites for electron capture gas-liquid chromatography
AU - Lau, Derick H
AU - Henderson, Gary L.
PY - 1976/12/22
Y1 - 1976/12/22
N2 - Six reagents-trichloroacetyl chloride, trichloroacetic anhydride, pentafluorobenzoyl chloride, heptafluorobutyryl chloride, heptafluorobutyric anhydride, and trifluoroacetic anhydride-were evaluated as potential derivatizing reagents for quantitating the metabolites of l-α-acetylmethadol (LAAM)-noracetylmethadol, dinoracetylmethadol, methadol, and normethadol-by electron capture gas-liquid chromatography. All of the reagents studied reacted quantitatively with all of the metabolites except methadol; however, trichloroacetyl chloride was found to be the most satisfactory general reagent for analyzing these metabolites in biological fluids. A gas-liquid chromatographic method is presented which combines both flame ionization and electron capture detection for quantitating plasma and urine levels of methadone, l-α-acetylmethadol and its metabolites.
AB - Six reagents-trichloroacetyl chloride, trichloroacetic anhydride, pentafluorobenzoyl chloride, heptafluorobutyryl chloride, heptafluorobutyric anhydride, and trifluoroacetic anhydride-were evaluated as potential derivatizing reagents for quantitating the metabolites of l-α-acetylmethadol (LAAM)-noracetylmethadol, dinoracetylmethadol, methadol, and normethadol-by electron capture gas-liquid chromatography. All of the reagents studied reacted quantitatively with all of the metabolites except methadol; however, trichloroacetyl chloride was found to be the most satisfactory general reagent for analyzing these metabolites in biological fluids. A gas-liquid chromatographic method is presented which combines both flame ionization and electron capture detection for quantitating plasma and urine levels of methadone, l-α-acetylmethadol and its metabolites.
UR - http://www.scopus.com/inward/record.url?scp=0017093734&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017093734&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(00)87792-4
DO - 10.1016/S0021-9673(00)87792-4
M3 - Article
VL - 129
SP - 329
EP - 338
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - C
ER -