Comparative study of the commonly used virulence tests for laboratory diagnosis of ovine footrot caused by Dichelobacter nodosus in Australia

Om P. Dhungyel, Ashley E Hill, Navneet K. Dhand, Richard J. Whittington

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Footrot in sheep and goats is expressed as a spectrum of clinical entities ranging from benign, which is a self limiting interdigital dermatitis to highly virulent, in which severe under running of the horn of the hoof occurs. Interactions between the host, the virulence of the causative strain of Dichelobacter nodosus and environmental conditions determine the severity of the disease. Clinical diagnosis of virulent footrot, which a notifiable disease in some states of Australia, is not always straightforward. Therefore, the gelatin gel and elastase tests for protease activity, and the intA PCR test for an inserted genetic element in D. nodosus are commonly used to support or to confirm a clinical diagnosis. A comparative study of these laboratory tests with a large number of samples collected from 12 flocks of sheep with clinically virulent footrot was conducted. Based on the elastase test, 64% of the isolates tested were classified as virulent compared to 91% on the gelatin gel test and 41% according to the intA test. The agreement between the elastase and the gelatin gel test was low (kappa. =0.12) as were the agreements between other tests. Only about 21% of the isolates were virulent in all 3 tests. Therefore these tests on their own may not provide standard and reliable results and are likely to remain as supplementary tests for clinical diagnosis of the disease.

Original languageEnglish (US)
Pages (from-to)756-760
Number of pages5
JournalVeterinary Microbiology
Volume162
Issue number2-4
DOIs
StatePublished - Mar 23 2013
Externally publishedYes

Fingerprint

Dichelobacter nodosus
footrot
Clinical Laboratory Techniques
Pancreatic Elastase
Gelatin
Virulence
Sheep
virulence
Gels
Hoof and Claw
elastase
testing
Dermatitis
Horns
Goats
Running
gelatin
Peptide Hydrolases
gels
Polymerase Chain Reaction

Keywords

  • Elastase test
  • Footrot
  • Gelatin gel test
  • IntA PCR test
  • Virulence

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Comparative study of the commonly used virulence tests for laboratory diagnosis of ovine footrot caused by Dichelobacter nodosus in Australia. / Dhungyel, Om P.; Hill, Ashley E; Dhand, Navneet K.; Whittington, Richard J.

In: Veterinary Microbiology, Vol. 162, No. 2-4, 23.03.2013, p. 756-760.

Research output: Contribution to journalArticle

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