Comparative studies of the capsid precursor polypeptide P1 and the capsid protein VP1 cDNA vectors for DNA vaccination against foot-and-mouth disease virus

Ning Sun Yang, Jeng Hwan Wang, Ku Feng Lin, Chien Yu Wang, Suk Am Kim, Yu Ling Yang, Ming Hwa Jong, Tsun Yung Kuo, Shiow Suey Lai, R. Holland Cheng, Ming Tsair Chan, Shu Mei Liang

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Foot-and-mouth disease virus (FMDV) causes a severe livestock disease, and the virus is an interesting target for virology and vaccine studies. Materials and methods: Here we evaluated comparatively three different viral antigen-encoding DNA sequences, delivered via two physical means (i.e., gene gun delivery into skin and electroporation delivery into muscle), for naked DNA-mediated vaccination in a mouse system. Results: Both methods gave similar results, demonstrating commonality of the observed DNA vaccine effects. Immunization with a cDNA vector expressing the major viral antigen (VP1) alone routinely failed to induce the production of anti-VP1 or neutralizing antibodies in test mice. As a second approach, the plasmid L-VP1 that produces a transgenic membrane-anchored VP1 protein elicited a strong antibody response, but all test mice failed in the FMDV challenge experiment. In contrast, for mice immunized with the viral capsid precursor protein (P1) cDNA expression vector, both neutralizing antibodies and 80-100% protection in test mice were detected. Conclusions: This strategy of using the whole capsid precursor protein P1 cDNA for vaccination, intentionally without the use of virus-specific protease or other encoding genes for safety reasons, may thus be employed as a relevant experimental system for induction or upgrading of effective neutralizing antibody response, and as a convenient surrogate test system for DNA vaccination studies of FMDV and presumably other viral diseases.

Original languageEnglish (US)
Pages (from-to)708-717
Number of pages10
JournalJournal of Gene Medicine
Volume7
Issue number6
DOIs
StatePublished - Jun 2005
Externally publishedYes

Fingerprint

Foot-and-Mouth Disease Virus
Capsid
Capsid Proteins
Vaccination
Complementary DNA
Peptides
Neutralizing Antibodies
DNA
Viral Antigens
Virus Diseases
Antibody Formation
Virology
DNA Vaccines
Electroporation
Firearms
Livestock
Viral Proteins
Genes
Anti-Idiotypic Antibodies
Immunization

Keywords

  • Capsid protein
  • DNA vaccine
  • FMDV
  • Immunogenicity
  • Viral clearance

ASJC Scopus subject areas

  • Genetics

Cite this

Comparative studies of the capsid precursor polypeptide P1 and the capsid protein VP1 cDNA vectors for DNA vaccination against foot-and-mouth disease virus. / Yang, Ning Sun; Wang, Jeng Hwan; Lin, Ku Feng; Wang, Chien Yu; Kim, Suk Am; Yang, Yu Ling; Jong, Ming Hwa; Kuo, Tsun Yung; Lai, Shiow Suey; Cheng, R. Holland; Chan, Ming Tsair; Liang, Shu Mei.

In: Journal of Gene Medicine, Vol. 7, No. 6, 06.2005, p. 708-717.

Research output: Contribution to journalArticle

Yang, NS, Wang, JH, Lin, KF, Wang, CY, Kim, SA, Yang, YL, Jong, MH, Kuo, TY, Lai, SS, Cheng, RH, Chan, MT & Liang, SM 2005, 'Comparative studies of the capsid precursor polypeptide P1 and the capsid protein VP1 cDNA vectors for DNA vaccination against foot-and-mouth disease virus', Journal of Gene Medicine, vol. 7, no. 6, pp. 708-717. https://doi.org/10.1002/jgm.723
Yang, Ning Sun ; Wang, Jeng Hwan ; Lin, Ku Feng ; Wang, Chien Yu ; Kim, Suk Am ; Yang, Yu Ling ; Jong, Ming Hwa ; Kuo, Tsun Yung ; Lai, Shiow Suey ; Cheng, R. Holland ; Chan, Ming Tsair ; Liang, Shu Mei. / Comparative studies of the capsid precursor polypeptide P1 and the capsid protein VP1 cDNA vectors for DNA vaccination against foot-and-mouth disease virus. In: Journal of Gene Medicine. 2005 ; Vol. 7, No. 6. pp. 708-717.
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abstract = "Background: Foot-and-mouth disease virus (FMDV) causes a severe livestock disease, and the virus is an interesting target for virology and vaccine studies. Materials and methods: Here we evaluated comparatively three different viral antigen-encoding DNA sequences, delivered via two physical means (i.e., gene gun delivery into skin and electroporation delivery into muscle), for naked DNA-mediated vaccination in a mouse system. Results: Both methods gave similar results, demonstrating commonality of the observed DNA vaccine effects. Immunization with a cDNA vector expressing the major viral antigen (VP1) alone routinely failed to induce the production of anti-VP1 or neutralizing antibodies in test mice. As a second approach, the plasmid L-VP1 that produces a transgenic membrane-anchored VP1 protein elicited a strong antibody response, but all test mice failed in the FMDV challenge experiment. In contrast, for mice immunized with the viral capsid precursor protein (P1) cDNA expression vector, both neutralizing antibodies and 80-100{\%} protection in test mice were detected. Conclusions: This strategy of using the whole capsid precursor protein P1 cDNA for vaccination, intentionally without the use of virus-specific protease or other encoding genes for safety reasons, may thus be employed as a relevant experimental system for induction or upgrading of effective neutralizing antibody response, and as a convenient surrogate test system for DNA vaccination studies of FMDV and presumably other viral diseases.",
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AU - Kim, Suk Am

AU - Yang, Yu Ling

AU - Jong, Ming Hwa

AU - Kuo, Tsun Yung

AU - Lai, Shiow Suey

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AU - Chan, Ming Tsair

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