Comparative studies of T = 3 and T = 4 icosahedral RNA insect viruses.

J. E. Johnson, S. Munshi, L. Liljas, D. Agrawal, N. H. Olson, V. Reddy, Andrew J Fisher, B. McKinney, T. Schmidt, T. S. Baker

Research output: Contribution to journalArticle

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Abstract

Crystallographic and molecular biological studies of T = 3 nodaviruses (180 identical subunits in the particle) and T = 4 tetraviruses (240 identical subunits in the particle) have revealed similarity in both the architecture of the particles and the strategy for maturation. The comparative studies provide a novel opportunity to examine an apparent evolution of particle size, from smaller (T = 3) to larger (T = 4), with both particles based on similar subunits. The BBV and FHV nodavirus structures are refined at 2.8 A and 3 A respectively, while the N omega V structure is at 6 A resolution. Nevertheless, the detailed comparisons of the noda and tetravirus X-ray electron density maps show that the same type of switching in subunit twofold contacts is used in the T = 3 and T = 4 capsids, although differences must exist between quasi and icosahedral threefold contacts in the T = 4 particle that have not yet been detected. The analyses of primary and tertiary structures of noda and tetraviruses show that N omega V subunits undergo a post assembly cleavage like that observed in nodaviruses and that the cleaved 76 C-terminal residues remain associated with the particle.

Original languageEnglish (US)
Pages (from-to)497-512
Number of pages16
JournalArchives of virology. Supplementum
Volume9
StatePublished - 1994
Externally publishedYes

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Insect Viruses
Capsid
RNA Viruses
Particle Size
X-Rays
Electrons

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

Johnson, J. E., Munshi, S., Liljas, L., Agrawal, D., Olson, N. H., Reddy, V., ... Baker, T. S. (1994). Comparative studies of T = 3 and T = 4 icosahedral RNA insect viruses. Archives of virology. Supplementum, 9, 497-512.

Comparative studies of T = 3 and T = 4 icosahedral RNA insect viruses. / Johnson, J. E.; Munshi, S.; Liljas, L.; Agrawal, D.; Olson, N. H.; Reddy, V.; Fisher, Andrew J; McKinney, B.; Schmidt, T.; Baker, T. S.

In: Archives of virology. Supplementum, Vol. 9, 1994, p. 497-512.

Research output: Contribution to journalArticle

Johnson, JE, Munshi, S, Liljas, L, Agrawal, D, Olson, NH, Reddy, V, Fisher, AJ, McKinney, B, Schmidt, T & Baker, TS 1994, 'Comparative studies of T = 3 and T = 4 icosahedral RNA insect viruses.', Archives of virology. Supplementum, vol. 9, pp. 497-512.
Johnson JE, Munshi S, Liljas L, Agrawal D, Olson NH, Reddy V et al. Comparative studies of T = 3 and T = 4 icosahedral RNA insect viruses. Archives of virology. Supplementum. 1994;9:497-512.
Johnson, J. E. ; Munshi, S. ; Liljas, L. ; Agrawal, D. ; Olson, N. H. ; Reddy, V. ; Fisher, Andrew J ; McKinney, B. ; Schmidt, T. ; Baker, T. S. / Comparative studies of T = 3 and T = 4 icosahedral RNA insect viruses. In: Archives of virology. Supplementum. 1994 ; Vol. 9. pp. 497-512.
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