Comparative structural and functional analysis of photoreceptor neurons of Rho-/- mice reveal increased survival on C57BL/6J in comparison to 129Sv genetic background

Marian M. Humphries, Sophie Kiang, Niamh McNally, Mary Anne Donovan, Paul A. Sieving, Ronald A. Bush, Shigeki Machida, Thomas Cotter, Audrey Hobson, Jane Farrar, Pete Humphries, Paul Kenna

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

To explore the possible influence of defined genetic backgrounds on photoreceptor viability and function in mice carrying a targeted disruption of the rhodopsin gene, the severities of retinopathies in Rho-/- mice on C57BL/6J and 129Sv congenic backgrounds were compared by light microscopy and electroretinography and qualitatively by in situ end labeling of DNA in apoptotic photoreceptor nuclei of retinal sections. Cone photoreceptor viability and function were shown to deteriorate more slowly on the C57BL/6J background in comparison to that of the 129Sv, with significantly greater numbers of outer nuclear layer nuclei in the retinas of C57BL/6J mice at 3 and 4 months of age. Both amplitude and waveform features of the ERG were shown to be remarkably different in the two strains, indicating an approximately 6-fold difference in C57BL/6J Rho-/- mice compared to 129Sv Rho-/- mice at 80 days. Thus, in comparison with the 129Sv strain, genetic modifiers appear to constitute a component of the C57BL/6J background, the expression of which significantly protects cone photoreceptors from apoptotic death in a mutation-induced murine retinopathy. The differences in phenotype revealed in this study are sufficient in principle to provide a basis for comparisons to be made between QTLs in light-induced and mutation-induced systems.

Original languageEnglish (US)
Pages (from-to)437-443
Number of pages7
JournalVisual Neuroscience
Volume18
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Electroretinogram
  • Genetic backgrounds
  • Modifiers
  • Mouse rhodopsin knockout

ASJC Scopus subject areas

  • Physiology
  • Sensory Systems

Fingerprint Dive into the research topics of 'Comparative structural and functional analysis of photoreceptor neurons of Rho-/- mice reveal increased survival on C57BL/6J in comparison to 129Sv genetic background'. Together they form a unique fingerprint.

Cite this