Comparative serum stability of radiochelates for antibody radiopharmaceuticals

W. C. Cole, S. J. DeNardo, C. F. Meares, M. J. McCall, A. L. Epstein, H. A. O'Brien, M. K. Moi

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Serum incubation of monoclonal antibodies chelate labeled by DPTA, benzyl-EDTA and benzyl-TETA with 111In, 57Co, and 67Cu demonstrated marked differences in their stability. In serum, 111In-benzyl-EDTA-antibody was more stable than 111In-DTPA-antibody. Cobalt-57 or 67Cu chelated antibody were less stable than either 111In chelated antibody; 67Cu was only firmly attached to the antibody as 67Cu-benzyl-TETA-antibody. The relative stability of the radiometal chelated antibodies was paralleled by the relative stability in serum of the radiometal chelates themselves. These in vitro studies suggest that in vivo behavior of metal chelates exposed to a complex protein environment cannot be predicted by classical equilibrium constants.

Original languageEnglish (US)
Pages (from-to)83-90
Number of pages8
JournalJournal of Nuclear Medicine
Volume28
Issue number1
StatePublished - 1987

Fingerprint

Radiopharmaceuticals
Antibodies
Serum
Pentetic Acid
Cobalt
Metals
Monoclonal Antibodies

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Cole, W. C., DeNardo, S. J., Meares, C. F., McCall, M. J., Epstein, A. L., O'Brien, H. A., & Moi, M. K. (1987). Comparative serum stability of radiochelates for antibody radiopharmaceuticals. Journal of Nuclear Medicine, 28(1), 83-90.

Comparative serum stability of radiochelates for antibody radiopharmaceuticals. / Cole, W. C.; DeNardo, S. J.; Meares, C. F.; McCall, M. J.; Epstein, A. L.; O'Brien, H. A.; Moi, M. K.

In: Journal of Nuclear Medicine, Vol. 28, No. 1, 1987, p. 83-90.

Research output: Contribution to journalArticle

Cole, WC, DeNardo, SJ, Meares, CF, McCall, MJ, Epstein, AL, O'Brien, HA & Moi, MK 1987, 'Comparative serum stability of radiochelates for antibody radiopharmaceuticals', Journal of Nuclear Medicine, vol. 28, no. 1, pp. 83-90.
Cole WC, DeNardo SJ, Meares CF, McCall MJ, Epstein AL, O'Brien HA et al. Comparative serum stability of radiochelates for antibody radiopharmaceuticals. Journal of Nuclear Medicine. 1987;28(1):83-90.
Cole, W. C. ; DeNardo, S. J. ; Meares, C. F. ; McCall, M. J. ; Epstein, A. L. ; O'Brien, H. A. ; Moi, M. K. / Comparative serum stability of radiochelates for antibody radiopharmaceuticals. In: Journal of Nuclear Medicine. 1987 ; Vol. 28, No. 1. pp. 83-90.
@article{8a3eb828852645b6a4242fbb8e83611b,
title = "Comparative serum stability of radiochelates for antibody radiopharmaceuticals",
abstract = "Serum incubation of monoclonal antibodies chelate labeled by DPTA, benzyl-EDTA and benzyl-TETA with 111In, 57Co, and 67Cu demonstrated marked differences in their stability. In serum, 111In-benzyl-EDTA-antibody was more stable than 111In-DTPA-antibody. Cobalt-57 or 67Cu chelated antibody were less stable than either 111In chelated antibody; 67Cu was only firmly attached to the antibody as 67Cu-benzyl-TETA-antibody. The relative stability of the radiometal chelated antibodies was paralleled by the relative stability in serum of the radiometal chelates themselves. These in vitro studies suggest that in vivo behavior of metal chelates exposed to a complex protein environment cannot be predicted by classical equilibrium constants.",
author = "Cole, {W. C.} and DeNardo, {S. J.} and Meares, {C. F.} and McCall, {M. J.} and Epstein, {A. L.} and O'Brien, {H. A.} and Moi, {M. K.}",
year = "1987",
language = "English (US)",
volume = "28",
pages = "83--90",
journal = "Journal of Nuclear Medicine",
issn = "0161-5505",
publisher = "Society of Nuclear Medicine Inc.",
number = "1",

}

TY - JOUR

T1 - Comparative serum stability of radiochelates for antibody radiopharmaceuticals

AU - Cole, W. C.

AU - DeNardo, S. J.

AU - Meares, C. F.

AU - McCall, M. J.

AU - Epstein, A. L.

AU - O'Brien, H. A.

AU - Moi, M. K.

PY - 1987

Y1 - 1987

N2 - Serum incubation of monoclonal antibodies chelate labeled by DPTA, benzyl-EDTA and benzyl-TETA with 111In, 57Co, and 67Cu demonstrated marked differences in their stability. In serum, 111In-benzyl-EDTA-antibody was more stable than 111In-DTPA-antibody. Cobalt-57 or 67Cu chelated antibody were less stable than either 111In chelated antibody; 67Cu was only firmly attached to the antibody as 67Cu-benzyl-TETA-antibody. The relative stability of the radiometal chelated antibodies was paralleled by the relative stability in serum of the radiometal chelates themselves. These in vitro studies suggest that in vivo behavior of metal chelates exposed to a complex protein environment cannot be predicted by classical equilibrium constants.

AB - Serum incubation of monoclonal antibodies chelate labeled by DPTA, benzyl-EDTA and benzyl-TETA with 111In, 57Co, and 67Cu demonstrated marked differences in their stability. In serum, 111In-benzyl-EDTA-antibody was more stable than 111In-DTPA-antibody. Cobalt-57 or 67Cu chelated antibody were less stable than either 111In chelated antibody; 67Cu was only firmly attached to the antibody as 67Cu-benzyl-TETA-antibody. The relative stability of the radiometal chelated antibodies was paralleled by the relative stability in serum of the radiometal chelates themselves. These in vitro studies suggest that in vivo behavior of metal chelates exposed to a complex protein environment cannot be predicted by classical equilibrium constants.

UR - http://www.scopus.com/inward/record.url?scp=0023132526&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023132526&partnerID=8YFLogxK

M3 - Article

C2 - 3794813

AN - SCOPUS:0023132526

VL - 28

SP - 83

EP - 90

JO - Journal of Nuclear Medicine

JF - Journal of Nuclear Medicine

SN - 0161-5505

IS - 1

ER -