Comparative sensitivity of measurements of lung damage made by bronchoalveolar lavage after short-term exposure of rats to ozone

Daniel J. Guth, Darren L. Warren, Jerold A Last

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Consequences of exposure of rats for 2 days or less to O3 at various concentrations between 0.12 and 0.96 ppm were measured using several assays performed on bronchoalveolar lavage fluid. Changes in apparent lung permeability were assessed by measurement of recovery of labelled bovine serum albumin in lung lavage fluid after intravenous injection ("permeability index"). The relative sensitivity of this assay was compared with the sensitivity of measurements of changes in protein and of enzyme content in lavage fluid. Permeability index increased in an exposure concentration-dependent manner after 6 or 24 h of exposure to O3 at or above levels of 0.4 ppm. Permeability index was also increased after 2 days of exposure to 0.2 ppm of O3. The activities of lactate dehydrogenase, acid phosphatase, and N-acetyl-β-D-glucosaminidase in lung lavage fluid were less sensitive indicators of O3 damage than was altered permeability index. Increased lactate dehydrogenase activity could only be detected after continuous exposure of rats for at least 1 day to 0.64 (or higher) ppm of O3, while acid phosphatase and N-acetyl-β-d-glucosaminidase activities were increased after exposure of rats to O3 at 0.4 ppm or above for 1 day. Activities of these enzymes were not increased after 6 h of exposure to 0.64 ppm of O3 or after 2 days of exposure to 0.2 ppm. Increased lavage protein content was the most sensitive measurement of the consequences of O3 exposure to rats in these protocols. The lavagable protein content increased after exposure of rats to O3 for 6 h at 0.4 ppm and for 1 or 2 days of exposure to 0.12 ppm, the current peak hourly National Ambient Air Quality standard for O3. While the biological significance of these observations remains to be determined, measurement of lavage protein content is a simple, sensitive indicator of acute changes in the lung caused by exposure to environmentally relevant concentrations of O3.

Original languageEnglish (US)
Pages (from-to)131-143
Number of pages13
JournalToxicology
Volume40
Issue number2
DOIs
StatePublished - 1986

Fingerprint

Ozone
Bronchoalveolar Lavage
Rats
Permeability
Therapeutic Irrigation
Bronchoalveolar Lavage Fluid
Lung
Hexosaminidases
Fluids
Acid Phosphatase
L-Lactate Dehydrogenase
Assays
Proteins
Air quality standards
Enzymes
Bovine Serum Albumin
Intravenous Injections
Air
Recovery

Keywords

  • Air pollution
  • Bronchoalveolar lavage
  • Lung
  • Ozone

ASJC Scopus subject areas

  • Toxicology

Cite this

Comparative sensitivity of measurements of lung damage made by bronchoalveolar lavage after short-term exposure of rats to ozone. / Guth, Daniel J.; Warren, Darren L.; Last, Jerold A.

In: Toxicology, Vol. 40, No. 2, 1986, p. 131-143.

Research output: Contribution to journalArticle

@article{56ce1c609c7e4acbb29a973b948bccce,
title = "Comparative sensitivity of measurements of lung damage made by bronchoalveolar lavage after short-term exposure of rats to ozone",
abstract = "Consequences of exposure of rats for 2 days or less to O3 at various concentrations between 0.12 and 0.96 ppm were measured using several assays performed on bronchoalveolar lavage fluid. Changes in apparent lung permeability were assessed by measurement of recovery of labelled bovine serum albumin in lung lavage fluid after intravenous injection ({"}permeability index{"}). The relative sensitivity of this assay was compared with the sensitivity of measurements of changes in protein and of enzyme content in lavage fluid. Permeability index increased in an exposure concentration-dependent manner after 6 or 24 h of exposure to O3 at or above levels of 0.4 ppm. Permeability index was also increased after 2 days of exposure to 0.2 ppm of O3. The activities of lactate dehydrogenase, acid phosphatase, and N-acetyl-β-D-glucosaminidase in lung lavage fluid were less sensitive indicators of O3 damage than was altered permeability index. Increased lactate dehydrogenase activity could only be detected after continuous exposure of rats for at least 1 day to 0.64 (or higher) ppm of O3, while acid phosphatase and N-acetyl-β-d-glucosaminidase activities were increased after exposure of rats to O3 at 0.4 ppm or above for 1 day. Activities of these enzymes were not increased after 6 h of exposure to 0.64 ppm of O3 or after 2 days of exposure to 0.2 ppm. Increased lavage protein content was the most sensitive measurement of the consequences of O3 exposure to rats in these protocols. The lavagable protein content increased after exposure of rats to O3 for 6 h at 0.4 ppm and for 1 or 2 days of exposure to 0.12 ppm, the current peak hourly National Ambient Air Quality standard for O3. While the biological significance of these observations remains to be determined, measurement of lavage protein content is a simple, sensitive indicator of acute changes in the lung caused by exposure to environmentally relevant concentrations of O3.",
keywords = "Air pollution, Bronchoalveolar lavage, Lung, Ozone",
author = "Guth, {Daniel J.} and Warren, {Darren L.} and Last, {Jerold A}",
year = "1986",
doi = "10.1016/0300-483X(86)90074-0",
language = "English (US)",
volume = "40",
pages = "131--143",
journal = "Toxicology",
issn = "0300-483X",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Comparative sensitivity of measurements of lung damage made by bronchoalveolar lavage after short-term exposure of rats to ozone

AU - Guth, Daniel J.

AU - Warren, Darren L.

AU - Last, Jerold A

PY - 1986

Y1 - 1986

N2 - Consequences of exposure of rats for 2 days or less to O3 at various concentrations between 0.12 and 0.96 ppm were measured using several assays performed on bronchoalveolar lavage fluid. Changes in apparent lung permeability were assessed by measurement of recovery of labelled bovine serum albumin in lung lavage fluid after intravenous injection ("permeability index"). The relative sensitivity of this assay was compared with the sensitivity of measurements of changes in protein and of enzyme content in lavage fluid. Permeability index increased in an exposure concentration-dependent manner after 6 or 24 h of exposure to O3 at or above levels of 0.4 ppm. Permeability index was also increased after 2 days of exposure to 0.2 ppm of O3. The activities of lactate dehydrogenase, acid phosphatase, and N-acetyl-β-D-glucosaminidase in lung lavage fluid were less sensitive indicators of O3 damage than was altered permeability index. Increased lactate dehydrogenase activity could only be detected after continuous exposure of rats for at least 1 day to 0.64 (or higher) ppm of O3, while acid phosphatase and N-acetyl-β-d-glucosaminidase activities were increased after exposure of rats to O3 at 0.4 ppm or above for 1 day. Activities of these enzymes were not increased after 6 h of exposure to 0.64 ppm of O3 or after 2 days of exposure to 0.2 ppm. Increased lavage protein content was the most sensitive measurement of the consequences of O3 exposure to rats in these protocols. The lavagable protein content increased after exposure of rats to O3 for 6 h at 0.4 ppm and for 1 or 2 days of exposure to 0.12 ppm, the current peak hourly National Ambient Air Quality standard for O3. While the biological significance of these observations remains to be determined, measurement of lavage protein content is a simple, sensitive indicator of acute changes in the lung caused by exposure to environmentally relevant concentrations of O3.

AB - Consequences of exposure of rats for 2 days or less to O3 at various concentrations between 0.12 and 0.96 ppm were measured using several assays performed on bronchoalveolar lavage fluid. Changes in apparent lung permeability were assessed by measurement of recovery of labelled bovine serum albumin in lung lavage fluid after intravenous injection ("permeability index"). The relative sensitivity of this assay was compared with the sensitivity of measurements of changes in protein and of enzyme content in lavage fluid. Permeability index increased in an exposure concentration-dependent manner after 6 or 24 h of exposure to O3 at or above levels of 0.4 ppm. Permeability index was also increased after 2 days of exposure to 0.2 ppm of O3. The activities of lactate dehydrogenase, acid phosphatase, and N-acetyl-β-D-glucosaminidase in lung lavage fluid were less sensitive indicators of O3 damage than was altered permeability index. Increased lactate dehydrogenase activity could only be detected after continuous exposure of rats for at least 1 day to 0.64 (or higher) ppm of O3, while acid phosphatase and N-acetyl-β-d-glucosaminidase activities were increased after exposure of rats to O3 at 0.4 ppm or above for 1 day. Activities of these enzymes were not increased after 6 h of exposure to 0.64 ppm of O3 or after 2 days of exposure to 0.2 ppm. Increased lavage protein content was the most sensitive measurement of the consequences of O3 exposure to rats in these protocols. The lavagable protein content increased after exposure of rats to O3 for 6 h at 0.4 ppm and for 1 or 2 days of exposure to 0.12 ppm, the current peak hourly National Ambient Air Quality standard for O3. While the biological significance of these observations remains to be determined, measurement of lavage protein content is a simple, sensitive indicator of acute changes in the lung caused by exposure to environmentally relevant concentrations of O3.

KW - Air pollution

KW - Bronchoalveolar lavage

KW - Lung

KW - Ozone

UR - http://www.scopus.com/inward/record.url?scp=0022510261&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022510261&partnerID=8YFLogxK

U2 - 10.1016/0300-483X(86)90074-0

DO - 10.1016/0300-483X(86)90074-0

M3 - Article

C2 - 3726890

AN - SCOPUS:0022510261

VL - 40

SP - 131

EP - 143

JO - Toxicology

JF - Toxicology

SN - 0300-483X

IS - 2

ER -