Abstract
Ribotyping and susceptibility to 17 antimicrobial agents were used to compare 37 isolates of Corynebacterium pseudotuberculosis (28 from horses, 1 from cattle, 3 from sheep and 5 from goats) derived from various types of lesions, and different geographic locations. According to the presence of nitrate reductase, all but one isolate from horses reduced nitrate (nitrate-positive), whereas all isolates from sheep and Seats were unable to reduce nitrate (nitrate-negative). The ribotype of the nitrate-negative isolate from a horse with ulcerative lymphangitis was identical to all the other isolates from horses, and different than the ribotype of nitrate-negative isolates from sheep and goats. Ribotyping with one of the restriction endonucleoses, Apa I, revealed differences between, but not within, the two biotypes. However, ribotyping with Pst I endonuclease revealed one variant within the equine biotype and one variant within the ovine biotype. The minimum inhibitory concentration (MIC; μg/ml) of antimicrobial agents against isolates from nitrate-negative and nitrate-positive groups was very similar, with the exception of isolates from sheep and goats which had a higher MIC for amikacin than isolates from horses and cattle.
Original language | English (US) |
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Pages (from-to) | 135-143 |
Number of pages | 9 |
Journal | Veterinary Microbiology |
Volume | 62 |
Issue number | 2 |
DOIs | |
State | Published - May 1 1998 |
Keywords
- 16S ribosomal RNA
- Corynebacterium pseudotuberculosis
- Horse
- RNA probes
ASJC Scopus subject areas
- Animal Science and Zoology
- Microbiology
- veterinary(all)