Comparative molecular characterization of Cornybacterium pseudotuberculosis of different origin

Lais R R Costa, Sharon Spier, Dwight C. Hirsh

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Ribotyping and susceptibility to 17 antimicrobial agents were used to compare 37 isolates of Corynebacterium pseudotuberculosis (28 from horses, 1 from cattle, 3 from sheep and 5 from goats) derived from various types of lesions, and different geographic locations. According to the presence of nitrate reductase, all but one isolate from horses reduced nitrate (nitrate-positive), whereas all isolates from sheep and Seats were unable to reduce nitrate (nitrate-negative). The ribotype of the nitrate-negative isolate from a horse with ulcerative lymphangitis was identical to all the other isolates from horses, and different than the ribotype of nitrate-negative isolates from sheep and goats. Ribotyping with one of the restriction endonucleoses, Apa I, revealed differences between, but not within, the two biotypes. However, ribotyping with Pst I endonuclease revealed one variant within the equine biotype and one variant within the ovine biotype. The minimum inhibitory concentration (MIC; μg/ml) of antimicrobial agents against isolates from nitrate-negative and nitrate-positive groups was very similar, with the exception of isolates from sheep and goats which had a higher MIC for amikacin than isolates from horses and cattle.

Original languageEnglish (US)
Pages (from-to)135-143
Number of pages9
JournalVeterinary Microbiology
Volume62
Issue number2
DOIs
StatePublished - May 1 1998

Keywords

  • 16S ribosomal RNA
  • Corynebacterium pseudotuberculosis
  • Horse
  • RNA probes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

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