Comparative large-scale analysis of interactions between several crop species and the effector repertoires from multiple pathovars of pseudomonas and Ralstonia

Tadeusz Wroblewski, Katherine S. Caldwell, Urszula Piskurewicz, Keri A. Cavanaugh, Huaqin Xu, Alexander Kozik, Oswaldo Ochoa, Leah K. Mchale, Kirsten Lahre, Joanna Jelenska, Jose A. Castillo, Daniel Blumenthal, Boris A. Vinatzer, Jean T. Greenberg, Richard W Michelmore

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Bacterial plant pathogens manipulate their hosts by injection of numerous effector proteins into host cells via type III secretion systems. Recognition of these effectors by the host plant leads to the induction of a defense reaction that often culminates in a hypersensitive response manifested as cell death. Genes encoding effector proteins can be exchanged between different strains of bacteria via horizontal transfer, and often individual strains are capable of infecting multiple hosts. Host plant species express diverse repertoires of resistance proteins that mediate direct or indirect recognition of bacterial effectors. As a result, plants and their bacterial pathogens should be considered as two extensive coevolving groups rather than as individual host species coevolving with single pathovars. To dissect the complexity of this coevolution, we cloned 171 effector-encoding genes from several pathovars of Pseudomonas and Ralstonia. We used Agrobacterium tumefaciens-mediated transient assays to test the ability of each effector to induce a necrotic phenotype on 59 plant genotypes belonging to four plant families, including numerous diverse accessions of lettuce (Lactuca sativa) and tomato (Solanum lycopersicum). Known defense-inducing effectors (avirulence factors) and their homologs commonly induced extensive necrosis in many different plant species. Nonhost species reacted to multiple effector proteins from an individual pathovar more frequently and more intensely than host species. Both homologous and sequence-unrelated effectors could elicit necrosis in a similar spectrum of plants, suggesting common effector targets or targeting of the same pathways in the plant cell.

Original languageEnglish (US)
Pages (from-to)1733-1749
Number of pages17
JournalPlant Physiology
Volume150
Issue number4
DOIs
StatePublished - Aug 2009

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Ralstonia
Pseudomonas
crops
Lettuce
necrosis
Lycopersicon esculentum
host plants
proteins
type III secretion system
Proteins
Necrosis
Solanum lycopersicum
Lactuca sativa
hypersensitive response
coevolution
Agrobacterium radiobacter
plant pathogens
Agrobacterium tumefaciens
lettuce
pathovars

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Comparative large-scale analysis of interactions between several crop species and the effector repertoires from multiple pathovars of pseudomonas and Ralstonia. / Wroblewski, Tadeusz; Caldwell, Katherine S.; Piskurewicz, Urszula; Cavanaugh, Keri A.; Xu, Huaqin; Kozik, Alexander; Ochoa, Oswaldo; Mchale, Leah K.; Lahre, Kirsten; Jelenska, Joanna; Castillo, Jose A.; Blumenthal, Daniel; Vinatzer, Boris A.; Greenberg, Jean T.; Michelmore, Richard W.

In: Plant Physiology, Vol. 150, No. 4, 08.2009, p. 1733-1749.

Research output: Contribution to journalArticle

Wroblewski, T, Caldwell, KS, Piskurewicz, U, Cavanaugh, KA, Xu, H, Kozik, A, Ochoa, O, Mchale, LK, Lahre, K, Jelenska, J, Castillo, JA, Blumenthal, D, Vinatzer, BA, Greenberg, JT & Michelmore, RW 2009, 'Comparative large-scale analysis of interactions between several crop species and the effector repertoires from multiple pathovars of pseudomonas and Ralstonia', Plant Physiology, vol. 150, no. 4, pp. 1733-1749. https://doi.org/10.1104/pp.109.140251
Wroblewski, Tadeusz ; Caldwell, Katherine S. ; Piskurewicz, Urszula ; Cavanaugh, Keri A. ; Xu, Huaqin ; Kozik, Alexander ; Ochoa, Oswaldo ; Mchale, Leah K. ; Lahre, Kirsten ; Jelenska, Joanna ; Castillo, Jose A. ; Blumenthal, Daniel ; Vinatzer, Boris A. ; Greenberg, Jean T. ; Michelmore, Richard W. / Comparative large-scale analysis of interactions between several crop species and the effector repertoires from multiple pathovars of pseudomonas and Ralstonia. In: Plant Physiology. 2009 ; Vol. 150, No. 4. pp. 1733-1749.
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