Comparative genomics reveals tissue-specific regulation of prolactin receptor gene expression

Anke Schennink, Josephine F. Trott, Rodrigo Manjarin, Danielle G. Lemay, Bradley A. Freking, Russell C. Hovey

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Prolactin (PRL), acting via the PRL receptor (PRLR), controls hundreds of biological processes across a range of species. Endocrine PRL elicits well-documented effects on target tissues such as the mammary glands and reproductive organs in addition to coordinating wholebody homeostasis during states such as lactation or adaptive responses to the environment. While changes in PRLR expression likely facilitates these tissue-specific responses to circulating PRL, the mechanisms regulating this regulation in non-rodent species has received limited attention. We performed a wide-scale analysis of PRLR 50 transcriptional regulation in pig tissues. Apart from the abundantly expressed and widely conserved exon 1, we identified alternative splicing of transcripts from an additional nine first exons of the porcine PRLR (pPRLR) gene. Notably, exon 1.5 transcripts were expressed most abundantly in the heart, while expression of exon 1.3-containing transcripts was greatest in the kidneys and small intestine. Expression of exon 1.3 mRNAs within the kidneys was most abundant in the renal cortex, and increased during gestation. A comparative analysis revealed a human homologue to exon 1.3, hE1N2, which was also principally transcribed in the kidneys and small intestines, and an exon hE1N3 was only expressed in the kidneys of humans. Promoter alignment revealed conserved motifs within the proximal promoter upstream of exon 1.3, including putative binding sites for hepatocyte nuclear factor-1 and Sp1. Together, these results highlight the diverse, conserved and tissue-specific regulation of PRLR expression in the targets for PRL, which may function to coordinate complex physiological states such as lactation and osmoregulation.

Original languageEnglish (US)
Pages (from-to)1-15
Number of pages15
JournalJournal of Molecular Endocrinology
Volume54
Issue number1
DOIs
StatePublished - Oct 30 2014

Fingerprint

Prolactin Receptors
Genomics
Exons
Gene Expression
Prolactin
Kidney
Swine
Lactation
Small Intestine
Hepatocyte Nuclear Factor 1
Biological Phenomena
Osmoregulation
Alternative Splicing
Human Mammary Glands
Homeostasis
Binding Sites
Pregnancy
Messenger RNA

Keywords

  • Gene regulation
  • Kidney
  • Prolactin
  • Receptor
  • Transcription factors

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology

Cite this

Schennink, A., Trott, J. F., Manjarin, R., Lemay, D. G., Freking, B. A., & Hovey, R. C. (2014). Comparative genomics reveals tissue-specific regulation of prolactin receptor gene expression. Journal of Molecular Endocrinology, 54(1), 1-15. https://doi.org/10.1530/JME-14-0212

Comparative genomics reveals tissue-specific regulation of prolactin receptor gene expression. / Schennink, Anke; Trott, Josephine F.; Manjarin, Rodrigo; Lemay, Danielle G.; Freking, Bradley A.; Hovey, Russell C.

In: Journal of Molecular Endocrinology, Vol. 54, No. 1, 30.10.2014, p. 1-15.

Research output: Contribution to journalArticle

Schennink, A, Trott, JF, Manjarin, R, Lemay, DG, Freking, BA & Hovey, RC 2014, 'Comparative genomics reveals tissue-specific regulation of prolactin receptor gene expression', Journal of Molecular Endocrinology, vol. 54, no. 1, pp. 1-15. https://doi.org/10.1530/JME-14-0212
Schennink, Anke ; Trott, Josephine F. ; Manjarin, Rodrigo ; Lemay, Danielle G. ; Freking, Bradley A. ; Hovey, Russell C. / Comparative genomics reveals tissue-specific regulation of prolactin receptor gene expression. In: Journal of Molecular Endocrinology. 2014 ; Vol. 54, No. 1. pp. 1-15.
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