Comparative genomics of the genus porphyromonas identifies adaptations for heme synthesis within the prevalent canine oral species porphyromonas cangingivalis

Ciaran O'Flynn, Oliver Deusch, Aaron E. Darling, Jonathan A Eisen, Corrin Wallis, Ian J. Davis, Stephen J. Harris

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Porphyromonads play an important role inhuman periodontal disease and recently have been shownto be highly prevalent in canine mouths. Porphyromonas cangingivalis is the most prevalent canine oral bacterial species in both plaque from healthy gingiva and plaque from dogs with early periodontitis. The ability of P. cangingivalis to flourish in the different environmental conditions characterized by these two states suggests a degree of metabolic flexibility. To characterize the genes responsible for this, the genomes of 32 isolates (including 18 newly sequenced and assembled) from18Porphyromonad species fromdogs, humans, and other mammals were compared. Phylogenetic trees inferred using core genes largely matched previous findings; however, comparative genomic analysis identified several genes and pathways relating to heme synthesis that were present in P. cangingivalis but not in other Porphyromonads. Porphyromonas cangingivalis has a complete protoporphyrin IX synthesis pathway potentially allowing it to synthesize its own heme unlike pathogenic Porphyromonads such as Porphyromonas gingivalis that acquire heme predominantly from blood. Other pathway differences such as the ability to synthesize siroheme and vitamin B12 point to enhanced metabolic flexibility for P. cangingivalis, which may underlie its prevalence in the canine oral cavity.

Original languageEnglish (US)
Pages (from-to)3397-3413
Number of pages17
JournalGenome Biology and Evolution
Volume7
Issue number12
DOIs
StatePublished - 2015

Keywords

  • Canine
  • Comparative genomics
  • Periodontal disease
  • Plaque
  • Porphyromonas

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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