Comparative genomics of emerging human ehrlichiosis agents.

Julie C. Dunning Hotopp, Mingqun Lin, Ramana Madupu, Jonathan Crabtree, Samuel V. Angiuoli, Jonathan A Eisen, Rekha Seshadri, Qinghu Ren, Martin Wu, Teresa R. Utterback, Shannon Smith, Matthew Lewis, Hoda Khouri, Chunbin Zhang, Hua Niu, Quan Lin, Norio Ohashi, Ning Zhi, William Nelson, Lauren M. Brinkac & 20 others Robert J. Dodson, M. J. Rosovitz, Jaideep Sundaram, Sean C. Daugherty, Tanja Davidsen, Anthony S. Durkin, Michelle Gwinn, Daniel H. Haft, Jeremy D. Selengut, Steven A. Sullivan, Nikhat Zafar, Liwei Zhou, Faiza Benahmed, Heather Forberger, Rebecca Halpin, Stephanie Mulligan, Jeffrey Robinson, Owen White, Yasuko Rikihisa, Hervé Tettelin

Research output: Contribution to journalArticle

277 Citations (Scopus)

Abstract

Anaplasma (formerly Ehrlichia) phagocytophilum, Ehrlichia chaffeensis, and Neorickettsia (formerly Ehrlichia) sennetsu are intracellular vector-borne pathogens that cause human ehrlichiosis, an emerging infectious disease. We present the complete genome sequences of these organisms along with comparisons to other organisms in the Rickettsiales order. Ehrlichia spp. and Anaplasma spp. display a unique large expansion of immunodominant outer membrane proteins facilitating antigenic variation. All Rickettsiales have a diminished ability to synthesize amino acids compared to their closest free-living relatives. Unlike members of the Rickettsiaceae family, these pathogenic Anaplasmataceae are capable of making all major vitamins, cofactors, and nucleotides, which could confer a beneficial role in the invertebrate vector or the vertebrate host. Further analysis identified proteins potentially involved in vacuole confinement of the Anaplasmataceae, a life cycle involving a hematophagous vector, vertebrate pathogenesis, human pathogenesis, and lack of transovarial transmission. These discoveries provide significant insights into the biology of these obligate intracellular pathogens.

Original languageEnglish (US)
JournalPLoS Genetics
Volume2
Issue number2
DOIs
StatePublished - Feb 2006
Externally publishedYes

Fingerprint

Anaplasmataceae
Anaplasma
Ehrlichiosis
Rickettsiales
ehrlichiosis
Genomics
Neorickettsia sennetsu
Neorickettsia
Rickettsiaceae
Vertebrates
genomics
Ehrlichia chaffeensis
pathogenesis
vertebrates
Ehrlichia
Anaplasma phagocytophilum
transovarial transmission
Emerging Communicable Diseases
Antigenic Variation
antigenic variation

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Dunning Hotopp, J. C., Lin, M., Madupu, R., Crabtree, J., Angiuoli, S. V., Eisen, J. A., ... Tettelin, H. (2006). Comparative genomics of emerging human ehrlichiosis agents. PLoS Genetics, 2(2). https://doi.org/10.1371/journal.pgen.0020021

Comparative genomics of emerging human ehrlichiosis agents. / Dunning Hotopp, Julie C.; Lin, Mingqun; Madupu, Ramana; Crabtree, Jonathan; Angiuoli, Samuel V.; Eisen, Jonathan A; Seshadri, Rekha; Ren, Qinghu; Wu, Martin; Utterback, Teresa R.; Smith, Shannon; Lewis, Matthew; Khouri, Hoda; Zhang, Chunbin; Niu, Hua; Lin, Quan; Ohashi, Norio; Zhi, Ning; Nelson, William; Brinkac, Lauren M.; Dodson, Robert J.; Rosovitz, M. J.; Sundaram, Jaideep; Daugherty, Sean C.; Davidsen, Tanja; Durkin, Anthony S.; Gwinn, Michelle; Haft, Daniel H.; Selengut, Jeremy D.; Sullivan, Steven A.; Zafar, Nikhat; Zhou, Liwei; Benahmed, Faiza; Forberger, Heather; Halpin, Rebecca; Mulligan, Stephanie; Robinson, Jeffrey; White, Owen; Rikihisa, Yasuko; Tettelin, Hervé.

In: PLoS Genetics, Vol. 2, No. 2, 02.2006.

Research output: Contribution to journalArticle

Dunning Hotopp, JC, Lin, M, Madupu, R, Crabtree, J, Angiuoli, SV, Eisen, JA, Seshadri, R, Ren, Q, Wu, M, Utterback, TR, Smith, S, Lewis, M, Khouri, H, Zhang, C, Niu, H, Lin, Q, Ohashi, N, Zhi, N, Nelson, W, Brinkac, LM, Dodson, RJ, Rosovitz, MJ, Sundaram, J, Daugherty, SC, Davidsen, T, Durkin, AS, Gwinn, M, Haft, DH, Selengut, JD, Sullivan, SA, Zafar, N, Zhou, L, Benahmed, F, Forberger, H, Halpin, R, Mulligan, S, Robinson, J, White, O, Rikihisa, Y & Tettelin, H 2006, 'Comparative genomics of emerging human ehrlichiosis agents.', PLoS Genetics, vol. 2, no. 2. https://doi.org/10.1371/journal.pgen.0020021
Dunning Hotopp JC, Lin M, Madupu R, Crabtree J, Angiuoli SV, Eisen JA et al. Comparative genomics of emerging human ehrlichiosis agents. PLoS Genetics. 2006 Feb;2(2). https://doi.org/10.1371/journal.pgen.0020021
Dunning Hotopp, Julie C. ; Lin, Mingqun ; Madupu, Ramana ; Crabtree, Jonathan ; Angiuoli, Samuel V. ; Eisen, Jonathan A ; Seshadri, Rekha ; Ren, Qinghu ; Wu, Martin ; Utterback, Teresa R. ; Smith, Shannon ; Lewis, Matthew ; Khouri, Hoda ; Zhang, Chunbin ; Niu, Hua ; Lin, Quan ; Ohashi, Norio ; Zhi, Ning ; Nelson, William ; Brinkac, Lauren M. ; Dodson, Robert J. ; Rosovitz, M. J. ; Sundaram, Jaideep ; Daugherty, Sean C. ; Davidsen, Tanja ; Durkin, Anthony S. ; Gwinn, Michelle ; Haft, Daniel H. ; Selengut, Jeremy D. ; Sullivan, Steven A. ; Zafar, Nikhat ; Zhou, Liwei ; Benahmed, Faiza ; Forberger, Heather ; Halpin, Rebecca ; Mulligan, Stephanie ; Robinson, Jeffrey ; White, Owen ; Rikihisa, Yasuko ; Tettelin, Hervé. / Comparative genomics of emerging human ehrlichiosis agents. In: PLoS Genetics. 2006 ; Vol. 2, No. 2.
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abstract = "Anaplasma (formerly Ehrlichia) phagocytophilum, Ehrlichia chaffeensis, and Neorickettsia (formerly Ehrlichia) sennetsu are intracellular vector-borne pathogens that cause human ehrlichiosis, an emerging infectious disease. We present the complete genome sequences of these organisms along with comparisons to other organisms in the Rickettsiales order. Ehrlichia spp. and Anaplasma spp. display a unique large expansion of immunodominant outer membrane proteins facilitating antigenic variation. All Rickettsiales have a diminished ability to synthesize amino acids compared to their closest free-living relatives. Unlike members of the Rickettsiaceae family, these pathogenic Anaplasmataceae are capable of making all major vitamins, cofactors, and nucleotides, which could confer a beneficial role in the invertebrate vector or the vertebrate host. Further analysis identified proteins potentially involved in vacuole confinement of the Anaplasmataceae, a life cycle involving a hematophagous vector, vertebrate pathogenesis, human pathogenesis, and lack of transovarial transmission. These discoveries provide significant insights into the biology of these obligate intracellular pathogens.",
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AU - Dunning Hotopp, Julie C.

AU - Lin, Mingqun

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AU - Angiuoli, Samuel V.

AU - Eisen, Jonathan A

AU - Seshadri, Rekha

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AU - Wu, Martin

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AU - Smith, Shannon

AU - Lewis, Matthew

AU - Khouri, Hoda

AU - Zhang, Chunbin

AU - Niu, Hua

AU - Lin, Quan

AU - Ohashi, Norio

AU - Zhi, Ning

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AU - Sullivan, Steven A.

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AU - Zhou, Liwei

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AU - Forberger, Heather

AU - Halpin, Rebecca

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AU - Rikihisa, Yasuko

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