Comparative evaluation of extraction methods for simultaneous mass-spectrometric analysis of complex lipids and primary metabolites from human blood plasma

Do Yup Lee, Tobias Kind, Young Ran Yoon, Oliver Fiehn, Kwang Hyeon Liu

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Metabolomic results on human blood plasma largely depend on the sample preparation protocols employed for protein precipitation and metabolite extraction. Five different extraction methods were examined, which can be grouped into two categories, liquid-liquid extraction and protein precipitation methods, including long-standing protocols such as the Folch extraction and Bligh-Dyer extraction in comparison to modern methods such as the Matyash protocol and two global metabolite extraction methods. Extracts were subjected to analysis of blood plasma lipids and primary metabolites by using chip-based direct infusion nanoelectrospray tandem mass spectrometry and gas chromatography coupled to time-of- flight mass spectrometry, respectively. Optimal extraction schemes were evaluated based on the number of identified metabolites, extraction efficiency, compound diversity, reproducibility, and convenience for high-throughput sample preparations. Results showed that Folch and Matyash methods were equally valid and robust for lipidomic assessments while primary metabolites were better assessed by the protein precipitation methods with organic solvent mixtures.

Original languageEnglish (US)
Pages (from-to)7275-7286
Number of pages12
JournalAnalytical and Bioanalytical Chemistry
Volume406
Issue number28
DOIs
StatePublished - 2014

Keywords

  • Extraction method
  • GC-MS
  • Human blood plasma
  • Lipid profiling
  • Nanoelectrospray direct-infusion MS
  • Primary metabolite profiling

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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