Abstract
The efficacies of TACI-Ig, rhTNFR:Fc and Methotrexate were compared in collagen-induced arthritis (CIA) mice. Sixty animals were divided into six groups: TACI-Ig (9 mg/kg), rhTNFR:Fc (4 mg/kg), Methotrexate (2 mg/kg) and IgG-Fc (9 mg/kg) groups and were given medication for six weeks. Meanwhile, normal and CIA mice were given as control. The different efficacies of drugs were evaluated by the analyses of ankle joints and spleens pathology, cytokines, T and B lymphocytes subsets. TACI-Ig and rhTNFR:Fc reduced arthritis scores seven days later than that of Methotrexate. TACI-Ig and Methotrexate were superior to rhTNFR:Fc in reduction synovial hyperplasia and cell infiltration scores. The same result was observed for scores of spleens histopathology. TACI-Ig and Methotrexate presented higher efficacy than rhTNFR:Fc on B lymphocyte stimulator, but TACI-Ig was inferior to rhTNFR:Fc on TNF-alpha. TACI-Ig and Methotrexate could reduce significantly IgA and IgM, but rhTNFR:Fc had no effects on these immunoglobulins. TACI-Ig had more efficacy than rhTNFR:Fc in the decrease of CD4+CD154+ T cell. TACI-Ig and Methotrexate also reduced CD4+CD69+ T cell, rhTNFR:Fc had no effects on the T cell subset. TACI-Ig and Methotrexate were superior to rhTNFR:Fc on CD4+CD62L+T cells. TACI-Ig and Methotrexate could reduce CD19+IgD+ and CD19+CD21 + B cells, but rhTNFR:Fc had no obvious effect on above B cells subsets. TACI-Ig is as effective as rhTNFR:Fc and Methotrexate on CIA mice by ameliorating joint and spleen pathology, regulating T and B lymphocytes function, although different mechanisms among them. This study would be useful for treatment selection of rheumatoid arthritis in different pathological conditions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 113-123 |
| Number of pages | 11 |
| Journal | European Journal of Pharmacology |
| Volume | 708 |
| Issue number | 1-3 |
| DOIs | |
| State | Published - May 15 2013 |
| Externally published | Yes |
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Keywords
- B lymphocyte stimulator
- Collagen-induced arthritis
- Lymphocyte
- Methotrexate
- RhTNFR:Fc
- TACI-Ig
ASJC Scopus subject areas
- Pharmacology
Cite this
Comparative efficacy of TACI-Ig with TNF-alpha inhibitor and methotrexate in DBA/1 mice with collagen-induced arthritis. / Zhang, Lingling; Li, Peipei; Song, Shanshan; Liu, Yunjie; Wang, Qingtong; Chang, Yan; Wu, Yujing; Chen, Jingyu; Zhao, Wendi; Zhang, Yunfang; Zhou, Aiwu; Wei, Wei.
In: European Journal of Pharmacology, Vol. 708, No. 1-3, 15.05.2013, p. 113-123.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative efficacy of TACI-Ig with TNF-alpha inhibitor and methotrexate in DBA/1 mice with collagen-induced arthritis
AU - Zhang, Lingling
AU - Li, Peipei
AU - Song, Shanshan
AU - Liu, Yunjie
AU - Wang, Qingtong
AU - Chang, Yan
AU - Wu, Yujing
AU - Chen, Jingyu
AU - Zhao, Wendi
AU - Zhang, Yunfang
AU - Zhou, Aiwu
AU - Wei, Wei
PY - 2013/5/15
Y1 - 2013/5/15
N2 - The efficacies of TACI-Ig, rhTNFR:Fc and Methotrexate were compared in collagen-induced arthritis (CIA) mice. Sixty animals were divided into six groups: TACI-Ig (9 mg/kg), rhTNFR:Fc (4 mg/kg), Methotrexate (2 mg/kg) and IgG-Fc (9 mg/kg) groups and were given medication for six weeks. Meanwhile, normal and CIA mice were given as control. The different efficacies of drugs were evaluated by the analyses of ankle joints and spleens pathology, cytokines, T and B lymphocytes subsets. TACI-Ig and rhTNFR:Fc reduced arthritis scores seven days later than that of Methotrexate. TACI-Ig and Methotrexate were superior to rhTNFR:Fc in reduction synovial hyperplasia and cell infiltration scores. The same result was observed for scores of spleens histopathology. TACI-Ig and Methotrexate presented higher efficacy than rhTNFR:Fc on B lymphocyte stimulator, but TACI-Ig was inferior to rhTNFR:Fc on TNF-alpha. TACI-Ig and Methotrexate could reduce significantly IgA and IgM, but rhTNFR:Fc had no effects on these immunoglobulins. TACI-Ig had more efficacy than rhTNFR:Fc in the decrease of CD4+CD154+ T cell. TACI-Ig and Methotrexate also reduced CD4+CD69+ T cell, rhTNFR:Fc had no effects on the T cell subset. TACI-Ig and Methotrexate were superior to rhTNFR:Fc on CD4+CD62L+T cells. TACI-Ig and Methotrexate could reduce CD19+IgD+ and CD19+CD21 + B cells, but rhTNFR:Fc had no obvious effect on above B cells subsets. TACI-Ig is as effective as rhTNFR:Fc and Methotrexate on CIA mice by ameliorating joint and spleen pathology, regulating T and B lymphocytes function, although different mechanisms among them. This study would be useful for treatment selection of rheumatoid arthritis in different pathological conditions.
AB - The efficacies of TACI-Ig, rhTNFR:Fc and Methotrexate were compared in collagen-induced arthritis (CIA) mice. Sixty animals were divided into six groups: TACI-Ig (9 mg/kg), rhTNFR:Fc (4 mg/kg), Methotrexate (2 mg/kg) and IgG-Fc (9 mg/kg) groups and were given medication for six weeks. Meanwhile, normal and CIA mice were given as control. The different efficacies of drugs were evaluated by the analyses of ankle joints and spleens pathology, cytokines, T and B lymphocytes subsets. TACI-Ig and rhTNFR:Fc reduced arthritis scores seven days later than that of Methotrexate. TACI-Ig and Methotrexate were superior to rhTNFR:Fc in reduction synovial hyperplasia and cell infiltration scores. The same result was observed for scores of spleens histopathology. TACI-Ig and Methotrexate presented higher efficacy than rhTNFR:Fc on B lymphocyte stimulator, but TACI-Ig was inferior to rhTNFR:Fc on TNF-alpha. TACI-Ig and Methotrexate could reduce significantly IgA and IgM, but rhTNFR:Fc had no effects on these immunoglobulins. TACI-Ig had more efficacy than rhTNFR:Fc in the decrease of CD4+CD154+ T cell. TACI-Ig and Methotrexate also reduced CD4+CD69+ T cell, rhTNFR:Fc had no effects on the T cell subset. TACI-Ig and Methotrexate were superior to rhTNFR:Fc on CD4+CD62L+T cells. TACI-Ig and Methotrexate could reduce CD19+IgD+ and CD19+CD21 + B cells, but rhTNFR:Fc had no obvious effect on above B cells subsets. TACI-Ig is as effective as rhTNFR:Fc and Methotrexate on CIA mice by ameliorating joint and spleen pathology, regulating T and B lymphocytes function, although different mechanisms among them. This study would be useful for treatment selection of rheumatoid arthritis in different pathological conditions.
KW - B lymphocyte stimulator
KW - Collagen-induced arthritis
KW - Lymphocyte
KW - Methotrexate
KW - RhTNFR:Fc
KW - TACI-Ig
UR - http://www.scopus.com/inward/record.url?scp=84877044820&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84877044820&partnerID=8YFLogxK
U2 - 10.1016/j.ejphar.2013.02.025
DO - 10.1016/j.ejphar.2013.02.025
M3 - Article
VL - 708
SP - 113
EP - 123
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 1-3
ER -