Comparative distribution and metabolism of triamcinolone acetonide and cortisol in the rat embryomaternal unit

J. M. Rowland, Z. R. Althaus, W. Slikker, Andrew G Hendrickx

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Triamcinolone acetonide (TAC) is teratogenic in rats while cortisol has been reported as not teratogenic. The objective of this investigation was to determine whether this difference in teratogenicity could be due to a difference in the metabolism and distribution of the parent compound in the embryomaternal unit. 3H‐TAC and 14C‐cortisol were administered intramuscularly to pregnant rats on day 12 of gestation. These dams were killed at each of the following time points after injection: 0.5, 1, 3, 6 and 24 hr. Maternal plasma and embryos were analyzed by high performance liquid chromatography (HPLC) and liquid scintillation counting. The plasma concentration of parent TAC was significantly greater than that for parent cortisol at all time points. The plasma elimination half‐life for TAC, 86 min, was also calculated to be significantly longer than that for cortisol, 8 min. Furthermore, the percentage of total plasma radioactivity representing HPLC resolved TAC was much higher than that representing cortisol at all time points. The concentration of TAC in the embryos was significantly greater than for cortisol at all time points. The elimination half‐life for unchanged TAC in the embryos was 142 min compared to 22 min for cortisol. The percentage of total radioactivity in the embryos representing unchanged TAC was similar to that found in maternal plasma while the percentage of total radioactivity representing unchanged cortisol was much lower than that found in maternal plasma. These findings support the hypothesis that differences in the distribution and metabolism of the parent compound are a critical factor in determining the teratogenicity of that compond.

Original languageEnglish (US)
Pages (from-to)333-341
Number of pages9
JournalTeratology
Volume27
Issue number3
DOIs
StatePublished - 1983

Fingerprint

Triamcinolone Acetonide
Metabolism
Hydrocortisone
Rats
Plasmas
Radioactivity
Embryonic Structures
Mothers
High performance liquid chromatography
Half-Life
High Pressure Liquid Chromatography
Scintillation Counting
Scintillation
Dams
Pregnancy
Injections
Liquids

ASJC Scopus subject areas

  • Embryology
  • Toxicology
  • Developmental Biology
  • Health, Toxicology and Mutagenesis

Cite this

Comparative distribution and metabolism of triamcinolone acetonide and cortisol in the rat embryomaternal unit. / Rowland, J. M.; Althaus, Z. R.; Slikker, W.; Hendrickx, Andrew G.

In: Teratology, Vol. 27, No. 3, 1983, p. 333-341.

Research output: Contribution to journalArticle

Rowland, J. M. ; Althaus, Z. R. ; Slikker, W. ; Hendrickx, Andrew G. / Comparative distribution and metabolism of triamcinolone acetonide and cortisol in the rat embryomaternal unit. In: Teratology. 1983 ; Vol. 27, No. 3. pp. 333-341.
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