Abstract
MeIQx and PhIP are putative carcinogenic heterocyclic amines formed during the cooking of meat and fish. Using accelerator mass spectrometry, we have investigated the metabolism and macromolecule binding of 14C-labelled MeIQx and PhIP in human cancer patients compared to the rat. Following oral administration of MeIQx and PhIP, more DNA adducts were formed in human colon tissue compared with rats. Differences were also observed between rats and humans in the metabolite profile and urine excretion for these compounds. These results suggest humans metabolise heterocyclic amines differently to laboratory rodents and question their use as models of human risk. Copyright (C) 1999 Elsevier Science Ireland Ltd.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 161-165 |
| Number of pages | 5 |
| Journal | Cancer Letters |
| Volume | 143 |
| Issue number | 2 |
| DOIs | |
| State | Published - Sep 1999 |
| Externally published | Yes |
Fingerprint
Keywords
- Accelerator mass spectrometry
- Colorectal cancer
- DNA adduct
- Heterocyclic amine
ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
- Oncology
Cite this
Comparative biotransformation studies of MeIQx and PhIP in animal models and humans. / Garner, R. C.; Lightfoot, T. J.; Cupid, B. C.; Russell, D.; Coxhead, J. M.; Kutschera, W.; Priller, A.; Rom, W.; Steier, P.; Alexander, D. J.; Leveson, S. H.; Dingley, K. H.; Mauthe, R. J.; Turteltaub, Ken W.
In: Cancer Letters, Vol. 143, No. 2, 09.1999, p. 161-165.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative biotransformation studies of MeIQx and PhIP in animal models and humans
AU - Garner, R. C.
AU - Lightfoot, T. J.
AU - Cupid, B. C.
AU - Russell, D.
AU - Coxhead, J. M.
AU - Kutschera, W.
AU - Priller, A.
AU - Rom, W.
AU - Steier, P.
AU - Alexander, D. J.
AU - Leveson, S. H.
AU - Dingley, K. H.
AU - Mauthe, R. J.
AU - Turteltaub, Ken W
PY - 1999/9
Y1 - 1999/9
N2 - MeIQx and PhIP are putative carcinogenic heterocyclic amines formed during the cooking of meat and fish. Using accelerator mass spectrometry, we have investigated the metabolism and macromolecule binding of 14C-labelled MeIQx and PhIP in human cancer patients compared to the rat. Following oral administration of MeIQx and PhIP, more DNA adducts were formed in human colon tissue compared with rats. Differences were also observed between rats and humans in the metabolite profile and urine excretion for these compounds. These results suggest humans metabolise heterocyclic amines differently to laboratory rodents and question their use as models of human risk. Copyright (C) 1999 Elsevier Science Ireland Ltd.
AB - MeIQx and PhIP are putative carcinogenic heterocyclic amines formed during the cooking of meat and fish. Using accelerator mass spectrometry, we have investigated the metabolism and macromolecule binding of 14C-labelled MeIQx and PhIP in human cancer patients compared to the rat. Following oral administration of MeIQx and PhIP, more DNA adducts were formed in human colon tissue compared with rats. Differences were also observed between rats and humans in the metabolite profile and urine excretion for these compounds. These results suggest humans metabolise heterocyclic amines differently to laboratory rodents and question their use as models of human risk. Copyright (C) 1999 Elsevier Science Ireland Ltd.
KW - Accelerator mass spectrometry
KW - Colorectal cancer
KW - DNA adduct
KW - Heterocyclic amine
UR - http://www.scopus.com/inward/record.url?scp=0032806626&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032806626&partnerID=8YFLogxK
U2 - 10.1016/S0304-3835(99)00118-4
DO - 10.1016/S0304-3835(99)00118-4
M3 - Article
VL - 143
SP - 161
EP - 165
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 2
ER -