Comparative analysis of the diagnostic performance of six major Echinococcus granulosus antigens assessed in a double-blind, randomized multicenter study

Carmen Lorenzo, Henrique B. Ferreira, Karina M. Monteiro, Mara Rosenzvit, Laura Kamenetzky, Hector H. García, Yessika Vasquez, Cesar Naquira, Elizabeth Sánchez, Myriam Lorca, María Contreras, Jerold A Last, Gualberto G. González-Sapienza

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The serodiagnosis of hydatid disease is a valuable instrument for clinical diagnosis and epidemiological surveillance of high-risk populations. In the past decade a wealth of reports on the diagnostic performance of numerous antigens have been produced. However, their diagnostic value has been estimated under different conditions, using different serum collection, therefore precluding their direct comparison. Here we report an unbiased comparison of the same batch of six major E. granulosus antigens, namely, hydatid cyst fluid (HCF), native antigen B (AgB), two recombinant AgB subunits, an AgB-derived synthetic peptide, and recombinant cytosolic malate dehydrogenase from E. granulosus (EgMDH), against the same serum collection. The double-blind analysis was performed using a standardized protocol and receiver operating characteristic (ROC) data analysis by a network of six South American laboratories. High intercenter reproducibility was attained, and the intralaboratory analysis allowed the comparative ranking of the antigen panel. HCF, AgB, and its AgB8/1 subunit exhibited equivalent diagnostic efficiencies, 81.4% ± 0.5%, 81.3% ± 0.6%, and 81.9% ± 2.0%, respectively; with a more favorable balance toward specificity in the case of the last antigen. The diagnostic efficiencies for the other three antigens were 76.8% ± 6.8%, 69.1% ± 2.7%, and 66.8% ± 2.1%, for the peptide, the AgB8/2 subunit, and the EgMDH, respectively. The study also included an analysis of batch-to-batch variation in the diagnostic performance of different HCF regional preparations. Based on these results, a suggested recommendation on the use of these antigens was drawn.

Original languageEnglish (US)
Pages (from-to)2764-2770
Number of pages7
JournalJournal of Clinical Microbiology
Volume43
Issue number6
DOIs
StatePublished - Jun 2005

Fingerprint

Echinococcus granulosus
Multicenter Studies
Antigens
Cyst Fluid
Echinococcosis
Malate Dehydrogenase
Peptides
Serologic Tests
Serum
ROC Curve

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Comparative analysis of the diagnostic performance of six major Echinococcus granulosus antigens assessed in a double-blind, randomized multicenter study. / Lorenzo, Carmen; Ferreira, Henrique B.; Monteiro, Karina M.; Rosenzvit, Mara; Kamenetzky, Laura; García, Hector H.; Vasquez, Yessika; Naquira, Cesar; Sánchez, Elizabeth; Lorca, Myriam; Contreras, María; Last, Jerold A; González-Sapienza, Gualberto G.

In: Journal of Clinical Microbiology, Vol. 43, No. 6, 06.2005, p. 2764-2770.

Research output: Contribution to journalArticle

Lorenzo, C, Ferreira, HB, Monteiro, KM, Rosenzvit, M, Kamenetzky, L, García, HH, Vasquez, Y, Naquira, C, Sánchez, E, Lorca, M, Contreras, M, Last, JA & González-Sapienza, GG 2005, 'Comparative analysis of the diagnostic performance of six major Echinococcus granulosus antigens assessed in a double-blind, randomized multicenter study', Journal of Clinical Microbiology, vol. 43, no. 6, pp. 2764-2770. https://doi.org/10.1128/JCM.43.6.2764-2770.2005
Lorenzo, Carmen ; Ferreira, Henrique B. ; Monteiro, Karina M. ; Rosenzvit, Mara ; Kamenetzky, Laura ; García, Hector H. ; Vasquez, Yessika ; Naquira, Cesar ; Sánchez, Elizabeth ; Lorca, Myriam ; Contreras, María ; Last, Jerold A ; González-Sapienza, Gualberto G. / Comparative analysis of the diagnostic performance of six major Echinococcus granulosus antigens assessed in a double-blind, randomized multicenter study. In: Journal of Clinical Microbiology. 2005 ; Vol. 43, No. 6. pp. 2764-2770.
@article{33360a73423941f789421db749ff5567,
title = "Comparative analysis of the diagnostic performance of six major Echinococcus granulosus antigens assessed in a double-blind, randomized multicenter study",
abstract = "The serodiagnosis of hydatid disease is a valuable instrument for clinical diagnosis and epidemiological surveillance of high-risk populations. In the past decade a wealth of reports on the diagnostic performance of numerous antigens have been produced. However, their diagnostic value has been estimated under different conditions, using different serum collection, therefore precluding their direct comparison. Here we report an unbiased comparison of the same batch of six major E. granulosus antigens, namely, hydatid cyst fluid (HCF), native antigen B (AgB), two recombinant AgB subunits, an AgB-derived synthetic peptide, and recombinant cytosolic malate dehydrogenase from E. granulosus (EgMDH), against the same serum collection. The double-blind analysis was performed using a standardized protocol and receiver operating characteristic (ROC) data analysis by a network of six South American laboratories. High intercenter reproducibility was attained, and the intralaboratory analysis allowed the comparative ranking of the antigen panel. HCF, AgB, and its AgB8/1 subunit exhibited equivalent diagnostic efficiencies, 81.4{\%} ± 0.5{\%}, 81.3{\%} ± 0.6{\%}, and 81.9{\%} ± 2.0{\%}, respectively; with a more favorable balance toward specificity in the case of the last antigen. The diagnostic efficiencies for the other three antigens were 76.8{\%} ± 6.8{\%}, 69.1{\%} ± 2.7{\%}, and 66.8{\%} ± 2.1{\%}, for the peptide, the AgB8/2 subunit, and the EgMDH, respectively. The study also included an analysis of batch-to-batch variation in the diagnostic performance of different HCF regional preparations. Based on these results, a suggested recommendation on the use of these antigens was drawn.",
author = "Carmen Lorenzo and Ferreira, {Henrique B.} and Monteiro, {Karina M.} and Mara Rosenzvit and Laura Kamenetzky and Garc{\'i}a, {Hector H.} and Yessika Vasquez and Cesar Naquira and Elizabeth S{\'a}nchez and Myriam Lorca and Mar{\'i}a Contreras and Last, {Jerold A} and Gonz{\'a}lez-Sapienza, {Gualberto G.}",
year = "2005",
month = "6",
doi = "10.1128/JCM.43.6.2764-2770.2005",
language = "English (US)",
volume = "43",
pages = "2764--2770",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "6",

}

TY - JOUR

T1 - Comparative analysis of the diagnostic performance of six major Echinococcus granulosus antigens assessed in a double-blind, randomized multicenter study

AU - Lorenzo, Carmen

AU - Ferreira, Henrique B.

AU - Monteiro, Karina M.

AU - Rosenzvit, Mara

AU - Kamenetzky, Laura

AU - García, Hector H.

AU - Vasquez, Yessika

AU - Naquira, Cesar

AU - Sánchez, Elizabeth

AU - Lorca, Myriam

AU - Contreras, María

AU - Last, Jerold A

AU - González-Sapienza, Gualberto G.

PY - 2005/6

Y1 - 2005/6

N2 - The serodiagnosis of hydatid disease is a valuable instrument for clinical diagnosis and epidemiological surveillance of high-risk populations. In the past decade a wealth of reports on the diagnostic performance of numerous antigens have been produced. However, their diagnostic value has been estimated under different conditions, using different serum collection, therefore precluding their direct comparison. Here we report an unbiased comparison of the same batch of six major E. granulosus antigens, namely, hydatid cyst fluid (HCF), native antigen B (AgB), two recombinant AgB subunits, an AgB-derived synthetic peptide, and recombinant cytosolic malate dehydrogenase from E. granulosus (EgMDH), against the same serum collection. The double-blind analysis was performed using a standardized protocol and receiver operating characteristic (ROC) data analysis by a network of six South American laboratories. High intercenter reproducibility was attained, and the intralaboratory analysis allowed the comparative ranking of the antigen panel. HCF, AgB, and its AgB8/1 subunit exhibited equivalent diagnostic efficiencies, 81.4% ± 0.5%, 81.3% ± 0.6%, and 81.9% ± 2.0%, respectively; with a more favorable balance toward specificity in the case of the last antigen. The diagnostic efficiencies for the other three antigens were 76.8% ± 6.8%, 69.1% ± 2.7%, and 66.8% ± 2.1%, for the peptide, the AgB8/2 subunit, and the EgMDH, respectively. The study also included an analysis of batch-to-batch variation in the diagnostic performance of different HCF regional preparations. Based on these results, a suggested recommendation on the use of these antigens was drawn.

AB - The serodiagnosis of hydatid disease is a valuable instrument for clinical diagnosis and epidemiological surveillance of high-risk populations. In the past decade a wealth of reports on the diagnostic performance of numerous antigens have been produced. However, their diagnostic value has been estimated under different conditions, using different serum collection, therefore precluding their direct comparison. Here we report an unbiased comparison of the same batch of six major E. granulosus antigens, namely, hydatid cyst fluid (HCF), native antigen B (AgB), two recombinant AgB subunits, an AgB-derived synthetic peptide, and recombinant cytosolic malate dehydrogenase from E. granulosus (EgMDH), against the same serum collection. The double-blind analysis was performed using a standardized protocol and receiver operating characteristic (ROC) data analysis by a network of six South American laboratories. High intercenter reproducibility was attained, and the intralaboratory analysis allowed the comparative ranking of the antigen panel. HCF, AgB, and its AgB8/1 subunit exhibited equivalent diagnostic efficiencies, 81.4% ± 0.5%, 81.3% ± 0.6%, and 81.9% ± 2.0%, respectively; with a more favorable balance toward specificity in the case of the last antigen. The diagnostic efficiencies for the other three antigens were 76.8% ± 6.8%, 69.1% ± 2.7%, and 66.8% ± 2.1%, for the peptide, the AgB8/2 subunit, and the EgMDH, respectively. The study also included an analysis of batch-to-batch variation in the diagnostic performance of different HCF regional preparations. Based on these results, a suggested recommendation on the use of these antigens was drawn.

UR - http://www.scopus.com/inward/record.url?scp=20444465255&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20444465255&partnerID=8YFLogxK

U2 - 10.1128/JCM.43.6.2764-2770.2005

DO - 10.1128/JCM.43.6.2764-2770.2005

M3 - Article

C2 - 15956395

AN - SCOPUS:20444465255

VL - 43

SP - 2764

EP - 2770

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 6

ER -