Abstract
1H NMR has detected both the deoxygenated proximal histidyl NδH signals of myoglobin (deoxyMb) and deoxygenated Hb (deoxyHb) from human gastrocnemius muscle. Exercising the muscle or pressure cuffing the leg to reduce blood flow elicits the appearance of the deoxyMb signal, which increases in intensity as cellular PO2 decreases. The deoxyMb signal is detected with a 45-s time resolution and reaches a steady-state level within 5 min of pressure cuffing. Its desaturation kinetics match those observed in the near-infrared spectroscopy (NIRS) experiments, implying that the NIRS signals are actually monitoring Mb desaturation. That interpretation is consistent with the signal intensity and desaturation of the deoxyHb proximal histidyl NδH signal from the β-subunit at 73 parts per million. The experimental results establish the feasibility and methodology to observe the deoxyMb and Hb signals in skeletal muscle, help clarify the origin of the NIRS signal, and set a stage for continuing study of O2 regulation in skeletal muscle.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Regulatory Integrative and Comparative Physiology |
| Volume | 276 |
| Issue number | 6 45-6 |
| State | Published - Jun 1999 |
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Keywords
- Bioenergetics
- Exercise
- Hemoglobin
- Myoglobin
- Near-infrared spectroscopy
- Oxygen
ASJC Scopus subject areas
- Physiology
- Physiology (medical)
Cite this
Comparative analysis of NMR and NIRS measurements of intracellular PO2 in human skeletal muscle. / Tran, Tuan Khanh; Sailasuta, Napapon; Kreutzer, Ulrike; Hurd, Ralph; Chung, Youngran; Mole, Paul; Kuno, Shinya; Jue, Thomas.
In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 276, No. 6 45-6, 06.1999.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative analysis of NMR and NIRS measurements of intracellular PO2 in human skeletal muscle
AU - Tran, Tuan Khanh
AU - Sailasuta, Napapon
AU - Kreutzer, Ulrike
AU - Hurd, Ralph
AU - Chung, Youngran
AU - Mole, Paul
AU - Kuno, Shinya
AU - Jue, Thomas
PY - 1999/6
Y1 - 1999/6
N2 - 1H NMR has detected both the deoxygenated proximal histidyl NδH signals of myoglobin (deoxyMb) and deoxygenated Hb (deoxyHb) from human gastrocnemius muscle. Exercising the muscle or pressure cuffing the leg to reduce blood flow elicits the appearance of the deoxyMb signal, which increases in intensity as cellular PO2 decreases. The deoxyMb signal is detected with a 45-s time resolution and reaches a steady-state level within 5 min of pressure cuffing. Its desaturation kinetics match those observed in the near-infrared spectroscopy (NIRS) experiments, implying that the NIRS signals are actually monitoring Mb desaturation. That interpretation is consistent with the signal intensity and desaturation of the deoxyHb proximal histidyl NδH signal from the β-subunit at 73 parts per million. The experimental results establish the feasibility and methodology to observe the deoxyMb and Hb signals in skeletal muscle, help clarify the origin of the NIRS signal, and set a stage for continuing study of O2 regulation in skeletal muscle.
AB - 1H NMR has detected both the deoxygenated proximal histidyl NδH signals of myoglobin (deoxyMb) and deoxygenated Hb (deoxyHb) from human gastrocnemius muscle. Exercising the muscle or pressure cuffing the leg to reduce blood flow elicits the appearance of the deoxyMb signal, which increases in intensity as cellular PO2 decreases. The deoxyMb signal is detected with a 45-s time resolution and reaches a steady-state level within 5 min of pressure cuffing. Its desaturation kinetics match those observed in the near-infrared spectroscopy (NIRS) experiments, implying that the NIRS signals are actually monitoring Mb desaturation. That interpretation is consistent with the signal intensity and desaturation of the deoxyHb proximal histidyl NδH signal from the β-subunit at 73 parts per million. The experimental results establish the feasibility and methodology to observe the deoxyMb and Hb signals in skeletal muscle, help clarify the origin of the NIRS signal, and set a stage for continuing study of O2 regulation in skeletal muscle.
KW - Bioenergetics
KW - Exercise
KW - Hemoglobin
KW - Myoglobin
KW - Near-infrared spectroscopy
KW - Oxygen
UR - http://www.scopus.com/inward/record.url?scp=0033067653&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033067653&partnerID=8YFLogxK
M3 - Article
C2 - 10362748
AN - SCOPUS:0033067653
VL - 276
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 1931-857X
IS - 6 45-6
ER -