Abstract
Boyce W. M., Branstetter B. A. and Kazacos K. R. 1988. Comparative analysis of larval excretory-secretory antigens of Baylisascaris procyonis, Toxocara canis and Ascaris suum by Western blotting and enzyme immunoassay. International Journal for Parasitology 18: 109-113. Larval excretorysecretory (ES) antigens were collected from in vitro cultures of Baylisascaris procyonis, Toxocara canis and Ascaris suum and fractionated by sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE). Following SDS-PAGE, specific antigens were identified on Western blots by enzyme immunoassay using a panel of antisera raised in mice. Serum was collected from mice infected with either Ascaris suum, Toxocara canis, Baylisascaris procyonis, Baylisascaris melis, Baylisascaris transfuga, Baylisascaris columnaris or Toxascaris leonina. Widespread cross-reactivity was detected among ascaridid antisera reacted against ES antigens from T. canis, B. procyonis and A. suum. However, certain B. procyonis ES antigens were recognized only by sera from mice infected with Baylisascaris spp., and these antigens may be useful for the immunodiagnosis of infections caused by members of this genus.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 109-113 |
| Number of pages | 5 |
| Journal | International Journal for Parasitology |
| Volume | 18 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1988 |
| Externally published | Yes |
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Keywords
- ascaridid nematodes
- Ascaris suum
- Baylisascaris procyonis
- excretory-secretory antigens
- immunoassay
- in vitro
- Toxocara canis
- Western blotting
ASJC Scopus subject areas
- Parasitology
- Infectious Diseases
Cite this
Comparative analysis of larval excretory-secretory antigens of Baylisascaris procyonis, Toxocara canis and Ascaris suum by Western blotting and enzyme immunoassay. / Boyce, Walter M; Branstetter, Brent A.; Kazacos, Kevin R.
In: International Journal for Parasitology, Vol. 18, No. 1, 1988, p. 109-113.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative analysis of larval excretory-secretory antigens of Baylisascaris procyonis, Toxocara canis and Ascaris suum by Western blotting and enzyme immunoassay
AU - Boyce, Walter M
AU - Branstetter, Brent A.
AU - Kazacos, Kevin R.
PY - 1988
Y1 - 1988
N2 - Boyce W. M., Branstetter B. A. and Kazacos K. R. 1988. Comparative analysis of larval excretory-secretory antigens of Baylisascaris procyonis, Toxocara canis and Ascaris suum by Western blotting and enzyme immunoassay. International Journal for Parasitology 18: 109-113. Larval excretorysecretory (ES) antigens were collected from in vitro cultures of Baylisascaris procyonis, Toxocara canis and Ascaris suum and fractionated by sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE). Following SDS-PAGE, specific antigens were identified on Western blots by enzyme immunoassay using a panel of antisera raised in mice. Serum was collected from mice infected with either Ascaris suum, Toxocara canis, Baylisascaris procyonis, Baylisascaris melis, Baylisascaris transfuga, Baylisascaris columnaris or Toxascaris leonina. Widespread cross-reactivity was detected among ascaridid antisera reacted against ES antigens from T. canis, B. procyonis and A. suum. However, certain B. procyonis ES antigens were recognized only by sera from mice infected with Baylisascaris spp., and these antigens may be useful for the immunodiagnosis of infections caused by members of this genus.
AB - Boyce W. M., Branstetter B. A. and Kazacos K. R. 1988. Comparative analysis of larval excretory-secretory antigens of Baylisascaris procyonis, Toxocara canis and Ascaris suum by Western blotting and enzyme immunoassay. International Journal for Parasitology 18: 109-113. Larval excretorysecretory (ES) antigens were collected from in vitro cultures of Baylisascaris procyonis, Toxocara canis and Ascaris suum and fractionated by sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE). Following SDS-PAGE, specific antigens were identified on Western blots by enzyme immunoassay using a panel of antisera raised in mice. Serum was collected from mice infected with either Ascaris suum, Toxocara canis, Baylisascaris procyonis, Baylisascaris melis, Baylisascaris transfuga, Baylisascaris columnaris or Toxascaris leonina. Widespread cross-reactivity was detected among ascaridid antisera reacted against ES antigens from T. canis, B. procyonis and A. suum. However, certain B. procyonis ES antigens were recognized only by sera from mice infected with Baylisascaris spp., and these antigens may be useful for the immunodiagnosis of infections caused by members of this genus.
KW - ascaridid nematodes
KW - Ascaris suum
KW - Baylisascaris procyonis
KW - excretory-secretory antigens
KW - immunoassay
KW - in vitro
KW - Toxocara canis
KW - Western blotting
UR - http://www.scopus.com/inward/record.url?scp=0023931261&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023931261&partnerID=8YFLogxK
U2 - 10.1016/0020-7519(88)90044-6
DO - 10.1016/0020-7519(88)90044-6
M3 - Article
C2 - 3366527
AN - SCOPUS:0023931261
VL - 18
SP - 109
EP - 113
JO - International Journal for Parasitology
JF - International Journal for Parasitology
SN - 0020-7519
IS - 1
ER -