Comparative analysis of larval excretory-secretory antigens of Baylisascaris procyonis, Toxocara canis and Ascaris suum by Western blotting and enzyme immunoassay

Walter M Boyce, Brent A. Branstetter, Kevin R. Kazacos

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Boyce W. M., Branstetter B. A. and Kazacos K. R. 1988. Comparative analysis of larval excretory-secretory antigens of Baylisascaris procyonis, Toxocara canis and Ascaris suum by Western blotting and enzyme immunoassay. International Journal for Parasitology 18: 109-113. Larval excretorysecretory (ES) antigens were collected from in vitro cultures of Baylisascaris procyonis, Toxocara canis and Ascaris suum and fractionated by sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE). Following SDS-PAGE, specific antigens were identified on Western blots by enzyme immunoassay using a panel of antisera raised in mice. Serum was collected from mice infected with either Ascaris suum, Toxocara canis, Baylisascaris procyonis, Baylisascaris melis, Baylisascaris transfuga, Baylisascaris columnaris or Toxascaris leonina. Widespread cross-reactivity was detected among ascaridid antisera reacted against ES antigens from T. canis, B. procyonis and A. suum. However, certain B. procyonis ES antigens were recognized only by sera from mice infected with Baylisascaris spp., and these antigens may be useful for the immunodiagnosis of infections caused by members of this genus.

Original languageEnglish (US)
Pages (from-to)109-113
Number of pages5
JournalInternational Journal for Parasitology
Volume18
Issue number1
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Ascaridoidea
Toxocara canis
Ascaris suum
Immunoenzyme Techniques
Western Blotting
Antigens
Polyacrylamide Gel Electrophoresis
Immune Sera
Toxascaris
Parasitology
Immunologic Tests
Serum
Sodium

Keywords

  • ascaridid nematodes
  • Ascaris suum
  • Baylisascaris procyonis
  • excretory-secretory antigens
  • immunoassay
  • in vitro
  • Toxocara canis
  • Western blotting

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

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title = "Comparative analysis of larval excretory-secretory antigens of Baylisascaris procyonis, Toxocara canis and Ascaris suum by Western blotting and enzyme immunoassay",
abstract = "Boyce W. M., Branstetter B. A. and Kazacos K. R. 1988. Comparative analysis of larval excretory-secretory antigens of Baylisascaris procyonis, Toxocara canis and Ascaris suum by Western blotting and enzyme immunoassay. International Journal for Parasitology 18: 109-113. Larval excretorysecretory (ES) antigens were collected from in vitro cultures of Baylisascaris procyonis, Toxocara canis and Ascaris suum and fractionated by sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE). Following SDS-PAGE, specific antigens were identified on Western blots by enzyme immunoassay using a panel of antisera raised in mice. Serum was collected from mice infected with either Ascaris suum, Toxocara canis, Baylisascaris procyonis, Baylisascaris melis, Baylisascaris transfuga, Baylisascaris columnaris or Toxascaris leonina. Widespread cross-reactivity was detected among ascaridid antisera reacted against ES antigens from T. canis, B. procyonis and A. suum. However, certain B. procyonis ES antigens were recognized only by sera from mice infected with Baylisascaris spp., and these antigens may be useful for the immunodiagnosis of infections caused by members of this genus.",
keywords = "ascaridid nematodes, Ascaris suum, Baylisascaris procyonis, excretory-secretory antigens, immunoassay, in vitro, Toxocara canis, Western blotting",
author = "Boyce, {Walter M} and Branstetter, {Brent A.} and Kazacos, {Kevin R.}",
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T1 - Comparative analysis of larval excretory-secretory antigens of Baylisascaris procyonis, Toxocara canis and Ascaris suum by Western blotting and enzyme immunoassay

AU - Boyce, Walter M

AU - Branstetter, Brent A.

AU - Kazacos, Kevin R.

PY - 1988

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N2 - Boyce W. M., Branstetter B. A. and Kazacos K. R. 1988. Comparative analysis of larval excretory-secretory antigens of Baylisascaris procyonis, Toxocara canis and Ascaris suum by Western blotting and enzyme immunoassay. International Journal for Parasitology 18: 109-113. Larval excretorysecretory (ES) antigens were collected from in vitro cultures of Baylisascaris procyonis, Toxocara canis and Ascaris suum and fractionated by sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE). Following SDS-PAGE, specific antigens were identified on Western blots by enzyme immunoassay using a panel of antisera raised in mice. Serum was collected from mice infected with either Ascaris suum, Toxocara canis, Baylisascaris procyonis, Baylisascaris melis, Baylisascaris transfuga, Baylisascaris columnaris or Toxascaris leonina. Widespread cross-reactivity was detected among ascaridid antisera reacted against ES antigens from T. canis, B. procyonis and A. suum. However, certain B. procyonis ES antigens were recognized only by sera from mice infected with Baylisascaris spp., and these antigens may be useful for the immunodiagnosis of infections caused by members of this genus.

AB - Boyce W. M., Branstetter B. A. and Kazacos K. R. 1988. Comparative analysis of larval excretory-secretory antigens of Baylisascaris procyonis, Toxocara canis and Ascaris suum by Western blotting and enzyme immunoassay. International Journal for Parasitology 18: 109-113. Larval excretorysecretory (ES) antigens were collected from in vitro cultures of Baylisascaris procyonis, Toxocara canis and Ascaris suum and fractionated by sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE). Following SDS-PAGE, specific antigens were identified on Western blots by enzyme immunoassay using a panel of antisera raised in mice. Serum was collected from mice infected with either Ascaris suum, Toxocara canis, Baylisascaris procyonis, Baylisascaris melis, Baylisascaris transfuga, Baylisascaris columnaris or Toxascaris leonina. Widespread cross-reactivity was detected among ascaridid antisera reacted against ES antigens from T. canis, B. procyonis and A. suum. However, certain B. procyonis ES antigens were recognized only by sera from mice infected with Baylisascaris spp., and these antigens may be useful for the immunodiagnosis of infections caused by members of this genus.

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KW - Toxocara canis

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