Comparable levels of microbial contamination in soil and on tomato crops after drip irrigation with treated wastewater or potable water

Ezra Orlofsky, Nirit Bernstein, Mollie Sacks, Ahuva Vonshak, Maya Benami, Arti Kundu, Michael Maki, Woutrina A Smith, Stefan Wuertz, Karen Shapiro, Osnat Gillor

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

To evaluate the impact of treated wastewater (TWW) irrigation for produce safety, field experiments were conducted to compare secondary and tertiary TWW with potable water using tomatoes as a model crop. Human pathogens including a suite of obligate and opportunistic bacterial pathogens (Campylobacter, Pseudomonas, Salmonella, Shigella, Staphylococcus), protozoa (Cryptosporidium and Giardia), and viruses (Adenovirus and Enterovirus) were monitored in two field trials using a combination of microscopic, cultivation-based, and molecular (qPCR) techniques. Results indicate that microbial contamination on the surface of tomatoes was not associated with the source of irrigation waters; fecal indicator bacteria (FIB) contamination was not statistically different on tomatoes irrigated with TWW or potable water. In fact, indicator bacteria testing did not predict the presence of pathogens in any of the matrices tested. Indicator bacteria and the opportunistic pathogens were detected in water, soil and on tomato surfaces from all irrigation treatment schemes, and were positively correlated with duration of time in the field (p

Original languageEnglish (US)
Pages (from-to)140-150
Number of pages11
JournalAgriculture, Ecosystems and Environment
Volume215
DOIs
StatePublished - Jan 1 2016

Fingerprint

drip irrigation
microbial contamination
soil pollution
microirrigation
drinking water
wastewater
pathogen
tomatoes
indicator species
crop
pathogens
irrigation
crops
bacterium
soil
wastewater irrigation
Enterovirus
Shigella
Giardia
Cryptosporidium

Keywords

  • Drip irrigation
  • Indicators
  • Pathogens
  • Potable water
  • Tomato
  • Treated wastewater

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Animal Science and Zoology
  • Ecology

Cite this

Comparable levels of microbial contamination in soil and on tomato crops after drip irrigation with treated wastewater or potable water. / Orlofsky, Ezra; Bernstein, Nirit; Sacks, Mollie; Vonshak, Ahuva; Benami, Maya; Kundu, Arti; Maki, Michael; Smith, Woutrina A; Wuertz, Stefan; Shapiro, Karen; Gillor, Osnat.

In: Agriculture, Ecosystems and Environment, Vol. 215, 01.01.2016, p. 140-150.

Research output: Contribution to journalArticle

Orlofsky, Ezra ; Bernstein, Nirit ; Sacks, Mollie ; Vonshak, Ahuva ; Benami, Maya ; Kundu, Arti ; Maki, Michael ; Smith, Woutrina A ; Wuertz, Stefan ; Shapiro, Karen ; Gillor, Osnat. / Comparable levels of microbial contamination in soil and on tomato crops after drip irrigation with treated wastewater or potable water. In: Agriculture, Ecosystems and Environment. 2016 ; Vol. 215. pp. 140-150.
@article{4b6edb25c6954c35b88d20a5807bceff,
title = "Comparable levels of microbial contamination in soil and on tomato crops after drip irrigation with treated wastewater or potable water",
abstract = "To evaluate the impact of treated wastewater (TWW) irrigation for produce safety, field experiments were conducted to compare secondary and tertiary TWW with potable water using tomatoes as a model crop. Human pathogens including a suite of obligate and opportunistic bacterial pathogens (Campylobacter, Pseudomonas, Salmonella, Shigella, Staphylococcus), protozoa (Cryptosporidium and Giardia), and viruses (Adenovirus and Enterovirus) were monitored in two field trials using a combination of microscopic, cultivation-based, and molecular (qPCR) techniques. Results indicate that microbial contamination on the surface of tomatoes was not associated with the source of irrigation waters; fecal indicator bacteria (FIB) contamination was not statistically different on tomatoes irrigated with TWW or potable water. In fact, indicator bacteria testing did not predict the presence of pathogens in any of the matrices tested. Indicator bacteria and the opportunistic pathogens were detected in water, soil and on tomato surfaces from all irrigation treatment schemes, and were positively correlated with duration of time in the field (p",
keywords = "Drip irrigation, Indicators, Pathogens, Potable water, Tomato, Treated wastewater",
author = "Ezra Orlofsky and Nirit Bernstein and Mollie Sacks and Ahuva Vonshak and Maya Benami and Arti Kundu and Michael Maki and Smith, {Woutrina A} and Stefan Wuertz and Karen Shapiro and Osnat Gillor",
year = "2016",
month = "1",
day = "1",
doi = "10.1016/j.agee.2015.08.008",
language = "English (US)",
volume = "215",
pages = "140--150",
journal = "Agriculture, Ecosystems and Environment",
issn = "0167-8809",
publisher = "Elsevier",

}

TY - JOUR

T1 - Comparable levels of microbial contamination in soil and on tomato crops after drip irrigation with treated wastewater or potable water

AU - Orlofsky, Ezra

AU - Bernstein, Nirit

AU - Sacks, Mollie

AU - Vonshak, Ahuva

AU - Benami, Maya

AU - Kundu, Arti

AU - Maki, Michael

AU - Smith, Woutrina A

AU - Wuertz, Stefan

AU - Shapiro, Karen

AU - Gillor, Osnat

PY - 2016/1/1

Y1 - 2016/1/1

N2 - To evaluate the impact of treated wastewater (TWW) irrigation for produce safety, field experiments were conducted to compare secondary and tertiary TWW with potable water using tomatoes as a model crop. Human pathogens including a suite of obligate and opportunistic bacterial pathogens (Campylobacter, Pseudomonas, Salmonella, Shigella, Staphylococcus), protozoa (Cryptosporidium and Giardia), and viruses (Adenovirus and Enterovirus) were monitored in two field trials using a combination of microscopic, cultivation-based, and molecular (qPCR) techniques. Results indicate that microbial contamination on the surface of tomatoes was not associated with the source of irrigation waters; fecal indicator bacteria (FIB) contamination was not statistically different on tomatoes irrigated with TWW or potable water. In fact, indicator bacteria testing did not predict the presence of pathogens in any of the matrices tested. Indicator bacteria and the opportunistic pathogens were detected in water, soil and on tomato surfaces from all irrigation treatment schemes, and were positively correlated with duration of time in the field (p

AB - To evaluate the impact of treated wastewater (TWW) irrigation for produce safety, field experiments were conducted to compare secondary and tertiary TWW with potable water using tomatoes as a model crop. Human pathogens including a suite of obligate and opportunistic bacterial pathogens (Campylobacter, Pseudomonas, Salmonella, Shigella, Staphylococcus), protozoa (Cryptosporidium and Giardia), and viruses (Adenovirus and Enterovirus) were monitored in two field trials using a combination of microscopic, cultivation-based, and molecular (qPCR) techniques. Results indicate that microbial contamination on the surface of tomatoes was not associated with the source of irrigation waters; fecal indicator bacteria (FIB) contamination was not statistically different on tomatoes irrigated with TWW or potable water. In fact, indicator bacteria testing did not predict the presence of pathogens in any of the matrices tested. Indicator bacteria and the opportunistic pathogens were detected in water, soil and on tomato surfaces from all irrigation treatment schemes, and were positively correlated with duration of time in the field (p

KW - Drip irrigation

KW - Indicators

KW - Pathogens

KW - Potable water

KW - Tomato

KW - Treated wastewater

UR - http://www.scopus.com/inward/record.url?scp=84943146509&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84943146509&partnerID=8YFLogxK

U2 - 10.1016/j.agee.2015.08.008

DO - 10.1016/j.agee.2015.08.008

M3 - Article

AN - SCOPUS:84943146509

VL - 215

SP - 140

EP - 150

JO - Agriculture, Ecosystems and Environment

JF - Agriculture, Ecosystems and Environment

SN - 0167-8809

ER -