Compact time-resolved laser-induced fluorescence spectroscopic system for clinical investigations of diseased tissue

Qiyin Fang, Thanassis Papaioannou, Javier Jo, Russel Vaitha, Kumar Shastry, Laura Marcu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this communication, we report the design, development, calibration, and characterization of a fluorescence spectroscopy instrument capable of measuring time-resolved fluorescence spectra from 350nm to 800nm with subnanosecond resolution. The compact system can be used in a variety of clinical setting including endoscopic procedures. The instrument can accomodate various types of laser sources and fiber optic probes for different types of applications. The typical acquisition time is about 0.8 s per wavelength or 40 s for a 200 nm time-resolvent fluorescence spectrum including online fluorescence lifetime analysis. The system has a variable spectral resolution from 0.1 to 10 nm and temporal resolution of 300 ps. Fluorescence spectra can be acquired with a sensitivity of 10 -9M at signal-to-noise ratio of 46. The fluorescence lifetimes of Rhodamin B and 9- cyanoanthracene were determined to be 2.92±0.12 ns and 12.28±0.12 ns indicating good agreement with literature values.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsT. Vo-Dinh, W.S. Grundfest, D.A. Benaron, G.E. Cohn
Pages60-66
Number of pages7
Volume4958
DOIs
StatePublished - 2003
EventPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Advanced Biomedical and Clinical Diagnostic Systems - San Jose, CA, United States
Duration: Jan 26 2003Jan 28 2003

Other

OtherPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Advanced Biomedical and Clinical Diagnostic Systems
CountryUnited States
CitySan Jose, CA
Period1/26/031/28/03

Keywords

  • Fiber optics
  • Lifetime measurement
  • Noninvasive tissue characterization
  • Time-resolved laser induced fluorescence

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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