Abstract
In this communication, we report the design, development, calibration, and characterization of a fluorescence spectroscopy instrument capable of measuring time-resolved fluorescence spectra from 350nm to 800nm with subnanosecond resolution. The compact system can be used in a variety of clinical setting including endoscopic procedures. The instrument can accomodate various types of laser sources and fiber optic probes for different types of applications. The typical acquisition time is about 0.8 s per wavelength or 40 s for a 200 nm time-resolvent fluorescence spectrum including online fluorescence lifetime analysis. The system has a variable spectral resolution from 0.1 to 10 nm and temporal resolution of 300 ps. Fluorescence spectra can be acquired with a sensitivity of 10 -9M at signal-to-noise ratio of 46. The fluorescence lifetimes of Rhodamin B and 9- cyanoanthracene were determined to be 2.92±0.12 ns and 12.28±0.12 ns indicating good agreement with literature values.
Original language | English (US) |
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Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
Editors | T. Vo-Dinh, W.S. Grundfest, D.A. Benaron, G.E. Cohn |
Pages | 60-66 |
Number of pages | 7 |
Volume | 4958 |
DOIs | |
State | Published - 2003 |
Event | PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Advanced Biomedical and Clinical Diagnostic Systems - San Jose, CA, United States Duration: Jan 26 2003 → Jan 28 2003 |
Other
Other | PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Advanced Biomedical and Clinical Diagnostic Systems |
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Country | United States |
City | San Jose, CA |
Period | 1/26/03 → 1/28/03 |
Keywords
- Fiber optics
- Lifetime measurement
- Noninvasive tissue characterization
- Time-resolved laser induced fluorescence
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics