Compact time-resolved laser-induced fluorescence spectroscopic system for clinical investigations of diseased tissue

Qiyin Fang; Thanassis Papaioannou; Javier Jo; Russel Vaitha; Kumar Shastry; Laura Marcu

doi:10.1117/12.476133

Compact time-resolved laser-induced fluorescence spectroscopic system for clinical investigations of diseased tissue

Qiyin Fang, Thanassis Papaioannou, Javier Jo, Russel Vaitha, Kumar Shastry, Laura Marcu

Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In this communication, we report the design, development, calibration, and characterization of a fluorescence spectroscopy instrument capable of measuring time-resolved fluorescence spectra from 350nm to 800nm with subnanosecond resolution. The compact system can be used in a variety of clinical setting including endoscopic procedures. The instrument can accomodate various types of laser sources and fiber optic probes for different types of applications. The typical acquisition time is about 0.8 s per wavelength or 40 s for a 200 nm time-resolvent fluorescence spectrum including online fluorescence lifetime analysis. The system has a variable spectral resolution from 0.1 to 10 nm and temporal resolution of 300 ps. Fluorescence spectra can be acquired with a sensitivity of 10 ^-9M at signal-to-noise ratio of 46. The fluorescence lifetimes of Rhodamin B and 9- cyanoanthracene were determined to be 2.92±0.12 ns and 12.28±0.12 ns indicating good agreement with literature values.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Editors	T. Vo-Dinh, W.S. Grundfest, D.A. Benaron, G.E. Cohn
Pages	60-66
Number of pages	7
Volume	4958
DOIs	https://doi.org/10.1117/12.476133
State	Published - 2003
Event	PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Advanced Biomedical and Clinical Diagnostic Systems - San Jose, CA, United States Duration: Jan 26 2003 → Jan 28 2003

Other

Other	PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Advanced Biomedical and Clinical Diagnostic Systems
Country/Territory	United States
City	San Jose, CA
Period	1/26/03 → 1/28/03

Keywords

Fiber optics
Lifetime measurement
Noninvasive tissue characterization
Time-resolved laser induced fluorescence

ASJC Scopus subject areas

Electrical and Electronic Engineering
Condensed Matter Physics

Access to Document

10.1117/12.476133

Cite this

Fang, Q., Papaioannou, T., Jo, J., Vaitha, R., Shastry, K., & Marcu, L. (2003). Compact time-resolved laser-induced fluorescence spectroscopic system for clinical investigations of diseased tissue. In T. Vo-Dinh, W. S. Grundfest, D. A. Benaron, & G. E. Cohn (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4958, pp. 60-66) https://doi.org/10.1117/12.476133

Compact time-resolved laser-induced fluorescence spectroscopic system for clinical investigations of diseased tissue. / Fang, Qiyin; Papaioannou, Thanassis; Jo, Javier; Vaitha, Russel; Shastry, Kumar; Marcu, Laura.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / T. Vo-Dinh; W.S. Grundfest; D.A. Benaron; G.E. Cohn. Vol. 4958 2003. p. 60-66.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Fang, Q, Papaioannou, T, Jo, J, Vaitha, R, Shastry, K & Marcu, L 2003, Compact time-resolved laser-induced fluorescence spectroscopic system for clinical investigations of diseased tissue. in T Vo-Dinh, WS Grundfest, DA Benaron & GE Cohn (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4958, pp. 60-66, PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Advanced Biomedical and Clinical Diagnostic Systems, San Jose, CA, United States, 1/26/03. https://doi.org/10.1117/12.476133

Fang, Qiyin ; Papaioannou, Thanassis ; Jo, Javier ; Vaitha, Russel ; Shastry, Kumar ; Marcu, Laura. / Compact time-resolved laser-induced fluorescence spectroscopic system for clinical investigations of diseased tissue. Proceedings of SPIE - The International Society for Optical Engineering. editor / T. Vo-Dinh ; W.S. Grundfest ; D.A. Benaron ; G.E. Cohn. Vol. 4958 2003. pp. 60-66

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abstract = "In this communication, we report the design, development, calibration, and characterization of a fluorescence spectroscopy instrument capable of measuring time-resolved fluorescence spectra from 350nm to 800nm with subnanosecond resolution. The compact system can be used in a variety of clinical setting including endoscopic procedures. The instrument can accomodate various types of laser sources and fiber optic probes for different types of applications. The typical acquisition time is about 0.8 s per wavelength or 40 s for a 200 nm time-resolvent fluorescence spectrum including online fluorescence lifetime analysis. The system has a variable spectral resolution from 0.1 to 10 nm and temporal resolution of 300 ps. Fluorescence spectra can be acquired with a sensitivity of 10 -9M at signal-to-noise ratio of 46. The fluorescence lifetimes of Rhodamin B and 9- cyanoanthracene were determined to be 2.92±0.12 ns and 12.28±0.12 ns indicating good agreement with literature values. ",

keywords = "Fiber optics, Lifetime measurement, Noninvasive tissue characterization, Time-resolved laser induced fluorescence",

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AB - In this communication, we report the design, development, calibration, and characterization of a fluorescence spectroscopy instrument capable of measuring time-resolved fluorescence spectra from 350nm to 800nm with subnanosecond resolution. The compact system can be used in a variety of clinical setting including endoscopic procedures. The instrument can accomodate various types of laser sources and fiber optic probes for different types of applications. The typical acquisition time is about 0.8 s per wavelength or 40 s for a 200 nm time-resolvent fluorescence spectrum including online fluorescence lifetime analysis. The system has a variable spectral resolution from 0.1 to 10 nm and temporal resolution of 300 ps. Fluorescence spectra can be acquired with a sensitivity of 10 -9M at signal-to-noise ratio of 46. The fluorescence lifetimes of Rhodamin B and 9- cyanoanthracene were determined to be 2.92±0.12 ns and 12.28±0.12 ns indicating good agreement with literature values.

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Compact time-resolved laser-induced fluorescence spectroscopic system for clinical investigations of diseased tissue

Abstract

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