Community-level differences in the microbiome of healthy wild mallards and those infected by influenza A viruses

Holly H. Ganz, Ladan Doroud, Alana J. Firl, Sarah M. Hird, Jonathan A Eisen, Walter M Boyce

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Waterfowl, especially ducks and geese, are primary reservoirs for influenza A viruses (IAVs) that evolve and emerge as important pathogens in domestic animals and humans. In contrast to humans, where IAVs infect the respiratory tract and cause significant morbidity and mortality, IAVs infect the gastrointestinal tract of waterfowl and cause little or no pathology and are spread by fecal-oral transmission. For this reason, we examined whether IAV infection is associated with differences in the cloacal microbiome of mallards (Anas platyrhyncos), an important host of IAVs in North America and Eurasia. We characterized bacterial community composition by sequencing the V4 region of 16S rRNA genes. IAV-positive mallards had lower species diversity, richness, and evenness than IAV-negative mallards. Operational taxonomic unit (OTU) cooccurrence patterns were also distinct depending on infection status. Network analysis showed that IAV-positive mallards had fewer significant cooccurring OTUs and exhibited fewer coassociation patterns among those OTUs than IAV-negative mallards. These results suggest that healthy mallards have a more robust and complex cloacal microbiome. By combining analytical approaches, we identified 41 bacterial OTUs, primarily representatives of Streptococcus spp., Veillonella dispar, and Rothia mucilaginosa, contributing to the observed differences. This study found that IAV-infected wild mallards exhibited strong differences in microbiome composition relative to noninfected mallards and identified a concise set of putative biomarker OTUs. Using Random Forest, a supervised machine learning method, we verified that these 41 bacterial OTUs are highly predictive of infection status.

Original languageEnglish (US)
Article numbere00188
JournalmSystems
Volume2
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

influenza
Influenza
Microbiota
Influenza A virus
Anas platyrhynchos
Viruses
Virus
virus
Otus
Infection
waterfowl
Veillonella dispar
Rothia mucilaginosa
Veillonella
microbiome
Community
infection
Geese
human influenza
Morbidity

Keywords

  • Biomarkers
  • Influenza
  • Machine learning
  • Mallard
  • Microbiome
  • Network modeling

ASJC Scopus subject areas

  • Molecular Biology
  • Physiology
  • Genetics
  • Biochemistry
  • Modeling and Simulation
  • Computer Science Applications
  • Ecology, Evolution, Behavior and Systematics
  • Microbiology

Cite this

Community-level differences in the microbiome of healthy wild mallards and those infected by influenza A viruses. / Ganz, Holly H.; Doroud, Ladan; Firl, Alana J.; Hird, Sarah M.; Eisen, Jonathan A; Boyce, Walter M.

In: mSystems, Vol. 2, No. 1, e00188, 01.01.2017.

Research output: Contribution to journalArticle

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