Common Commercial and Consumer Products Contain Activators of the Aryl Hydrocarbon (Dioxin) Receptor

Bin Zhao, Jessica E S Bohonowych, Alicia Timme-Laragy, Dawoon Jung, Alessandra A. Affatato, Robert H. Rice, Richard T. Di Giulio, Michael S. Denison

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Activation of the Ah receptor (AhR) by halogenated aromatic hydrocarbons (HAHs), such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, dioxin), can produce a wide variety of toxic and biological effects. While recent studies have shown that the AhR can bind and be activated by structurally diverse chemicals, how widespread of these AhR agonists are in environmental, biological and synthetic materials remains to be determined. Using AhR-based assays, we demonstrate the presence of potent AhR agonists in a variety of common commercial and consumer items. Solvent extracts of paper, rubber and plastic products contain chemicals that can bind to and stimulate AhR DNA binding and/or AhR-dependent gene expression in hepatic cytosol, cultured cell lines, human epidermis and zebrafish embryos. In contrast to TCDD and other persistent dioxin-like HAHs, activation of AhR-dependent gene expression by these extracts was transient, suggesting that the agonists are metabolically labile. Solvent extracts of rubber products produce AhR-dependent developmental toxicity in zebrafish in vivo, and inhibition of expression of the metabolic enzyme CYP1A, significantly increased their toxic potency. Although the identity of the responsible AhR-active chemicals and their toxicological impact remain to be determined, our data demonstrate that AhR active chemicals are widely distributed in everyday products.

Original languageEnglish (US)
Article numbere56860
JournalPLoS One
Volume8
Issue number2
DOIs
StatePublished - Feb 18 2013

Fingerprint

Aryl Hydrocarbon Receptors
dioxins
Consumer products
Hydrocarbons
Halogenated Hydrocarbons
hydrocarbons
Aromatic Hydrocarbons
Dioxins
Poisons
Zebrafish
Rubber products
receptors
Gene expression
Gene Expression
Chemical activation
Rubber
Plastic products
Paper products
Epidermis
Cytosol

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Zhao, B., Bohonowych, J. E. S., Timme-Laragy, A., Jung, D., Affatato, A. A., Rice, R. H., ... Denison, M. S. (2013). Common Commercial and Consumer Products Contain Activators of the Aryl Hydrocarbon (Dioxin) Receptor. PLoS One, 8(2), [e56860]. https://doi.org/10.1371/journal.pone.0056860

Common Commercial and Consumer Products Contain Activators of the Aryl Hydrocarbon (Dioxin) Receptor. / Zhao, Bin; Bohonowych, Jessica E S; Timme-Laragy, Alicia; Jung, Dawoon; Affatato, Alessandra A.; Rice, Robert H.; Di Giulio, Richard T.; Denison, Michael S.

In: PLoS One, Vol. 8, No. 2, e56860, 18.02.2013.

Research output: Contribution to journalArticle

Zhao, B, Bohonowych, JES, Timme-Laragy, A, Jung, D, Affatato, AA, Rice, RH, Di Giulio, RT & Denison, MS 2013, 'Common Commercial and Consumer Products Contain Activators of the Aryl Hydrocarbon (Dioxin) Receptor', PLoS One, vol. 8, no. 2, e56860. https://doi.org/10.1371/journal.pone.0056860
Zhao B, Bohonowych JES, Timme-Laragy A, Jung D, Affatato AA, Rice RH et al. Common Commercial and Consumer Products Contain Activators of the Aryl Hydrocarbon (Dioxin) Receptor. PLoS One. 2013 Feb 18;8(2). e56860. https://doi.org/10.1371/journal.pone.0056860
Zhao, Bin ; Bohonowych, Jessica E S ; Timme-Laragy, Alicia ; Jung, Dawoon ; Affatato, Alessandra A. ; Rice, Robert H. ; Di Giulio, Richard T. ; Denison, Michael S. / Common Commercial and Consumer Products Contain Activators of the Aryl Hydrocarbon (Dioxin) Receptor. In: PLoS One. 2013 ; Vol. 8, No. 2.
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