Combined pretrauma scopolamine and phencyclidine attenuate posttraumatic increased sensitivity to delayed secondary ischemia

L. W. Jenkins, Bruce G Lyeth, W. Lewelt, K. Moszynski, D. S. Dewitt, R. L. Balster, L. P. Miller, G. L. Clifton, H. F. Young, R. L. Hayes

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

Fasted Wistar rats were given a mild level of traumatic brain injury (TBI) and then subjected to 6 min of transient forebrain ischemia 24 h posttrauma. One group was given simultaneous 1 mg/kg scopolamine and 4 mg/kg phencyclidine intraperitoneally (IP) 15 min before trauma and another group an equal volume of plasmalyte A solution. After 7 days of postinjury survival, placebo-treated rats demonstrated increased posttraumatic vulnerability to secondary ischemic CA1 neuronal death even 24 h after trauma. This finding confirmed that increased posttraumatic ischemic vulnerability persists for at least 24 h even following mild trauma. Combined muscarinic receptor and N-methyl-D-aspartate (NMDA) receptor coupled ion channel blockade given and present during the mild TBI statistically attenuated this enhanced secondary ischemic CA1 neuronal death and thus posttraumatic increased ischemic vulnerability. Placebo-treated rats had 335.3 ± 93.6 CA1 neurons/106μm2 and drug-treated rats had 844.8 ± 184.9 CA1 neurons/106μm2. This result suggests that muscarinic and/or NMDA receptor-mediated events confined to TBI and the early posttraumatic period are in part responsible for the phenomenon of increased posttraumatic ischemic vulnerability.

Original languageEnglish (US)
Pages (from-to)275-287
Number of pages13
JournalJournal of Neurotrauma
Volume5
Issue number4
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Combined pretrauma scopolamine and phencyclidine attenuate posttraumatic increased sensitivity to delayed secondary ischemia'. Together they form a unique fingerprint.

  • Cite this

    Jenkins, L. W., Lyeth, B. G., Lewelt, W., Moszynski, K., Dewitt, D. S., Balster, R. L., Miller, L. P., Clifton, G. L., Young, H. F., & Hayes, R. L. (1988). Combined pretrauma scopolamine and phencyclidine attenuate posttraumatic increased sensitivity to delayed secondary ischemia. Journal of Neurotrauma, 5(4), 275-287.