Combined fiber probe for fluorescence lifetime and Raman spectroscopy

Sebastian Dochow, Dinglong Ma, Ines Latka, Thomas Bocklitz, Brad Hartl, Julien Bec, Hussain Fatakdawala, Eric Marple, Kirk Urmey, Sebastian Wachsmann-Hogiu, Michael Schmitt, Laura Marcu, Jürgen Popp

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

In this contribution we present a dual modality fiber optic probe combining fluorescence lifetime imaging (FLIm) and Raman spectroscopy for in vivo endoscopic applications. The presented multi-spectroscopy probe enables efficient excitation and collection of fluorescence lifetime signals for FLIm in the UV/visible wavelength region, as well as of Raman spectra in the near-IR for simultaneous Raman/FLIm imaging. The probe was characterized in terms of its lateral resolution and distance dependency of the Raman and FLIm signals. In addition, the feasibility of the probe for in vivo FLIm and Raman spectral characterization of tissue was demonstrated. Graphical Abstract An image comparison between FLIm and Raman spectroscopy acquired with the bimodal probe onseveral tissue samples.

Original languageEnglish (US)
Pages (from-to)8291-8301
Number of pages11
JournalAnalytical and Bioanalytical Chemistry
Volume407
Issue number27
DOIs
StatePublished - Nov 1 2015

Keywords

  • Bimodal
  • Fiber probes
  • Fluorescence lifetime
  • Imaging
  • Raman spectroscopy

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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    Dochow, S., Ma, D., Latka, I., Bocklitz, T., Hartl, B., Bec, J., Fatakdawala, H., Marple, E., Urmey, K., Wachsmann-Hogiu, S., Schmitt, M., Marcu, L., & Popp, J. (2015). Combined fiber probe for fluorescence lifetime and Raman spectroscopy. Analytical and Bioanalytical Chemistry, 407(27), 8291-8301. https://doi.org/10.1007/s00216-015-8800-5