Combinatorial Library Screening with Liposomes for Discovery of Membrane Active Peptides

Randy Carney, Yann Thillier, Zsofia Kiss, Amir Sahabi, Jean Carlos Heleno Campos, Alisha Knudson, Ruiwu Liu, David Olivos, Mary Saunders, Lin Tian, Kit Lam

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Membrane active peptides (MAPs) represent a class of short biomolecules that have shown great promise in facilitating intracellular delivery without disrupting cellular plasma membranes. Yet their clinical application has been stalled by numerous factors: off-target delivery, a requirement for high local concentration near cells of interest, degradation en route to the target site, and in the case of cell-penetrating peptides, eventual entrapment in endolysosomal compartments. The current method of deriving MAPs from naturally occurring proteins has restricted the discovery of new peptides that may overcome these limitations. Here, we describe a new branch of assays featuring high-throughput functional screening capable of discovering new peptides with tailored cell uptake and endosomal escape capabilities. The one-bead-one-compound (OBOC) combinatorial method is used to screen libraries containing millions of potential MAPs for binding to synthetic liposomes, which can be adapted to mimic various aspects of limiting membranes. By incorporating unnatural and d-amino acids in the library, in addition to varying buffer conditions and liposome compositions, we have identified several new highly potent MAPs that improve on current standards and introduce motifs that were previously unknown or considered unsuitable. Since small variations in pH and lipid composition can be controlled during screening, peptides discovered using this methodology could aid researchers building drug delivery platforms with unique requirements, such as targeted intracellular localization.

Original languageEnglish (US)
Pages (from-to)299-307
Number of pages9
JournalACS Combinatorial Science
Volume19
Issue number5
DOIs
StatePublished - May 8 2017

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Keywords

  • drug delivery platforms
  • endosomal escape capabilities
  • high-throughput
  • liposomes
  • membrane-active proteins
  • one-bead-one-compound

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Carney, R., Thillier, Y., Kiss, Z., Sahabi, A., Heleno Campos, J. C., Knudson, A., Liu, R., Olivos, D., Saunders, M., Tian, L., & Lam, K. (2017). Combinatorial Library Screening with Liposomes for Discovery of Membrane Active Peptides. ACS Combinatorial Science, 19(5), 299-307. https://doi.org/10.1021/acscombsci.6b00182